Only smiles in the tissue culture room from now on 🙂
Find the TK4 plasmids from the Wernig lab here:
www.addgene.org/browse/artic...

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis CRISPR screens in iPSC-derived neurons reveal that the E3 ubiquitin ligase CRL5SOCS4 ubiquitinates tau, that CUL5 expression is correlated with resilience in human Alzheimer’s disease, and that electron transport chain dysfunction alters tau degradation by the proteasome.

Now online! CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

Doing CRISPR - check out our PlatinumCRISPr upgrade thanks to @roliarnold.bsky.social and @ihaussmann.bsky.social
platinum-crispr.bham.ac.uk

Wonderful to see our paper on the #organelle signatures of #neurons and #astrocytes out in final form - congratulations, Shannon Rhoads and team!🎉 t.co/BPxKlbU6Ou

ABCA7 variants impact phosphatidylcholine and mitochondria in neurons - Nature Loss-of-function variants of ABCA7, associated with Alzheimer’s disease, result in disrupted lipid metabolism, mitochondrial function, DNA repair and synaptic signalling pathways in the human brain.

ABCA7 variants impact phosphatidylcholine and mitochondria in neurons www.nature.com/articles/s41...

Akayama, Shima, Nakamura et al. show that TFEB is regulated by at least two different regulatory mechanisms during lysosomal damage: ATG conjugation system–dependent/independent regulation. rupress.org/jcb/article/...

#Lysosomes #Autophagy

HHS Staff Response to the August 8th Attack — Save HHS Public servants and allies are standing together to demand work environments free from violence, accountability for leaders and policymakers who put everyday Americans in danger, and public health lea...

NEW: More than 750 current and former staff of HHS, including @CDCgov, and NIH, have signed a letter to RFKJr and members of Congress accusing him of endangering the lives of employees and the American people following the Aug 8 attack on the CDC.
www.savehhs.org/letter/hhs-s...

After CDC shooting, its employees turn their anger to RFK Jr. and Trump Investigators allege the shooter targeted the agency because of his coronavirus vaccine beliefs, according to two CDC officials briefed on the case.

NEW: The CDC shooting was much worse than initially reported. 181 rounds. 150 windows hit. Staff are furious at RFK Jr. and Trump becuz their long-running fears about anti-vax rhetoric turning deadly seem to be vindicated. @laurenweberhp.bluesky.social + Me.

www.washingtonpost.com/health/2025/...

Thank you all for the concern. We are all alive and OK-ish following the targeted attack on the CDC in Atlanta.

With boost to NIH budget, Senate panel rejects Trump’s plan to slash agency Republicans on spending committee push back against proposed reorganization and overhead cuts

The National Institutes of Health’s budget, and structure, would be preserved under a Senate bill for the 2026 fiscal year.

Astrocyte induction of disease-associated microglia is suppressed by acute exposure to fAD neurons in human iPSC triple cultures Lish et al. present a co-culture system of iPSC-derived human microglia, astrocytes, and neurons to investigate intercellular communication across different environmental and genetic contexts. Transcr...

New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...

Cocrystal structure reveals the mechanism of FSP1 inhibition by FSEN1 | PNAS FSP1 is an FAD-dependent oxidoreductase that uses NAD(P)H to regenerate the reduced forms of lipophilic quinone antioxidants, such as coenzyme Q10 ...

Thrilled this paper is out! We solved the first cocrystal structure of FSP1 with an inhibitor (FSEN1), providing mechanistic insight & a foundation for medchem. Led by Amalia Megarioti & Sitao Zhang. A terrific collaboration with Da Jia.
Brief Skytorial!
1/9

CLU alleviates Alzheimer’s disease-relevant processes by modulating astrocyte reactivity and microglia-dependent synaptic density Lish et al. reveal that AD-protective CLU alleles enhance CLU upregulation in response to accumulated neuropathology, thereby dampening inflammatory signaling between microglia and astrocytes. Using s...

New TYP lab paper out today in @cp-neuron.bsky.social led by PhD student @alexlish.bsky.social! We took a deep dive into the mechanisms underlying CLU in AD using innovative iPSC, mouse, and human data approaches. Be sure to give it a read!
www.cell.com/neuron/abstr...

Thank you, Scott Peters!

We appreciate you!

scottpeters.house.gov/2025/3/rep-p...

We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....

Atlanta Stand Up For Science March poster, State Capital, Liberty Plaza, March 7, 2025 noon-4

Stand Up For Science next Friday (3/7)! In 2024, Georgia institutions received just over $1 billion in funding and had more than $30 billion in economic impact in GA. That's a PHENOMENAL return for tax payers- let's share that news!

Supplements: too good to be true?

The dietary supplement industry in the United States is estimated to be worth $70 billion or more. The supplement market grew by 50% from 2018 to 2020. It is estimated that 100,000 unique supplements are currently available for purchase.

The FDA requires the following disclosure be displayed on the package of all supplements: "These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, or cure, or prevent any disease." Be skeptical of any supplement that is marketed with claims contrary to that disclosure. Always consult with your primary care provider before starting or stopping any supplement regimen.

If it seems too good to be true, it probably is.

Harvard Health reports on the recent ruling that the makers of Prevagen can no longer claim their #supplement improves #memory. The author recommends being wary about supplement marketing claims, which are often false, misleading, or based on insufficient research.

Facilities and Administrative (F&A) Costs Explainer YouTube video by Association of American Universities

Make sure you, your trainees, and anyone else who might listen to you understands what "indirects" actually are, how they are established, and what Universities contribute. H/T @rodallab.bsky.social

Please share widely!

www.youtube.com/watch?v=yxTD...

The attack on the NIH has begun | Science-Based Medicine Donald Trump has been sworn in, and his new administration turned its gaze on the NIH. The danger to US biomedical research has never been more acute.

Donald Trump has been sworn in, and his new administration has immediately turned its sights on the NIH. The danger to US biomedical research has never been more acute. sciencebasedmedicine.org/the-attack-o...

Started off this morning emailing my Senators and Congressman about the NIH and the potential damage being done by unnecessary disruptions.

They need to hear from us loud and clear. Constituents matter to them as one of the primary purposes of a politician is to get re-elected (and its their job).

Excited to share the lab's recent publication. Granulins are critical for lysosome function and brain health to prevent neurodegeneration. Our data suggest that granulin peptides could be used to treat frontotemporal dementia (FTD), Parkinson’s disease, Alzheimer’s disease.
t.co/pN28kabjIA

Great work from Mark Sands’ lab showing a 50% reduction in lysosomal enzymes puts you at risk for Alzheimer’s and other neurodegenerative diseases.

Very happy to share our paper on Familial British Dementia (FBD) which is online today!

Using patient iPSC,we show the amyloidogenic peptide in this disease -ABri -is mainly produced by microglia…

Never heard of FBD? You won’t be the only one… explainer incoming!

link.springer.com/article/10.1...

Deep proteomic analysis of microglia reveals fundamental biological differences between model systems Lloyd et al. generated a comprehensive microglia proteome database, identifying over 11,000 proteins. Fundamental differences between ex vivo and in vitro microglia associated with protein synthesis, ...

My latest paper, deep proteomic analysis of mouse and human microglia: www.cell.com/cell-reports...