The i2 seminars are back on Thursday, April 17, at 4 pm with two talks by Dr. Bea Casati, Postdoc in the Papavasiliou Lab, and Larissa Frank, PhD student in the Rodewald Lab. Save the date and join us for a lively discussion!
@dkfz.bsky.social
@beacasati.bsky.social
@larissafrank.bsky.social

Impressive findings, congrats! I also very much enjoyed the beautifully written piece „behind the paper“, sounds like an intense tour de force!

Beyond classical immunity: Mast cells as signal converters between tissues and neurons Mast cells act as “signal converters,” translating immunological and tissue signals into host-protective behaviors. They sense antigen and possibly toxins driving avoidance behavior, which can prevent...

'In this review, we discuss the communication between mast cells and neurons in host-protective avoidance behavior and expulsion reflexes and present a concept of mast cells as converters of signals from IgE and other stimuli into organ-specific or systemic reflexes.'
#Immunology #Neuroimmunology

Polytope: High-resolution epitope barcoding for in vivo spatial fate-mapping Tracing the fate of individual cells and their progeny remains a challenging task. While imaging-based fate-mapping provides spatial information, it generally lacks complexity due to limited label div...

Excited to share our preprint: Polytope, an epitope barcoding system, generating up to 512 unique color codes for in vivo lineage tracing. 🌈🧬 This tech enables spatial fate-mapping with unprecedented clonal and spatial resolution! doi.org/10.1101/2024... #SpatialBiology #LineageTracing #Polytope

Holistic genetic barcoding reveals a lineage tree of tissue macrophage development https://www.biorxiv.org/content/10.1101/2024.11.29.625985v1 Tissue macrophages are crucial for organ development and homeostasis, yet the developmental routes l

Holistic genetic barcoding reveals a lineage tree of tissue macrophage development www.biorxiv.org/content/10.1101/2024.11....

Holistic genetic barcoding reveals a lineage tree of tissue macrophage development Tissue macrophages are crucial for organ development and homeostasis, yet the developmental routes leading to tissue macrophages remain controversial. By combining unbiased Polylox barcoding with comp...

#macrophage #barcoding 🤯 Time to get rid of the "fetal liver monocyte" origin for resident macrophages?!? Congrats to the Rodewald lab for this work!
www.biorxiv.org/content/10.1...

Dream team collaboration with dpostrach.bsky.social
and Maurice Langhinrichs from the Höfer Lab!

We hope our method will be useful also for others who aim to resolve complex lineage pathways in an unbiased way.

And as a side note: Also #Polytope, the new spatial barcoding mouse by Daniel Postrach, takes the stage in our macrophage preprint!

Holistic genetic barcoding reveals a lineage tree of tissue macrophage development Tissue macrophages are crucial for organ development and homeostasis, yet the developmental routes leading to tissue macrophages remain controversial. By combining unbiased Polylox barcoding with comp...

🎉 Next preprint on its way!
#Polylox #barcoding + computational modelling meets #macrophage #development!

We used 𝐮𝐧𝐛𝐢𝐚𝐬𝐞𝐝 𝐏𝐨𝐥𝐲𝐥𝐨𝐱 𝐛𝐚𝐫𝐜𝐨𝐝𝐢𝐧𝐠 to trace thousands of embryonic progenitor clones and map their development into tissue-specific macrophages.

Happy reading!
doi.org/10.1101/2024...

#barcoding #polytope #imaging #lineagetracing

Let the new spatial barcoding era begin!
🚨𝐏𝐨𝐥𝐲𝐭𝐨𝐩𝐞 𝐢𝐬 𝐡𝐞𝐫𝐞!

New preprint by my colleague @dpostrach.bsky.social:
𝐏𝐨𝐥𝐲𝐭𝐨𝐩𝐞 uses 𝐮𝐩 𝐭𝐨 𝟓𝟏𝟐 𝐜𝐨𝐥𝐨𝐫𝐬 𝐭𝐨 𝐭𝐫𝐚𝐜𝐞 𝐜𝐥𝐨𝐧𝐞𝐬 𝐢𝐧 𝐬𝐢𝐭𝐮 as they grow, expand and travel.
And cheap readout using IBEX.

Can't wait to see all the fields that will profit from this tool!