Volume of mutant transmedulla neurons expressing membrane mCherry (🟦STAR RED) in adult #Drosophila brain. WT neurons labeled with GFP🟥. Isotropic #3DSTED with #MIRAVAPOLYSCOPE shows disorganized dendrites.
Thanks to Dr. Tzu-Yang Lin and Prof. Chi-Hon Lee (Academia Sinica, Taiwan) for the sample.
#Drosophila brain stained for transmedulla neurons with abberior STAR RED🟨 and fixed GFP🟦. Compared to confocal imaging, #3DSTED reveals higher resolution, enabling tracing of ultra-fine dendritic structures.
Thanks to Dr. Tzu-Yang Lin and Prof. Chi-Hon Lee (Academia Sinica, Taiwan) for the sample.
#ExM #3DSTED for #fluorescencefriday:
#herpesvirus particles 4-5x expanded. Viral glycoprotein (🟨STAR RED) and tegument protein (🟦STAR 580) stained.
Don’t just expand your sample, expand your options with abberior dyes and STED!
Sample courtesy: Jannik Jensen & @bosse-lab.bsky.social
#virology
#3DSTED volume of #Drosophila nephrocytes stained for Kirre🟦(STAR RED), nephrocyte diaphragm component (Neph1 homolog), and DAPI🟥. Light burden was reduced with #FLEXPOSURE adaptive illumination.
Sample courtesy: Konrad Lang, Freiburg Uni Medical Center, Germany
#fluorescencefriday #superresolution
BABB-cleared early #Xenopus embryos with stained nuclei (RNA Pol II pS2, STAR RED). The combination of #3DSTED and MATRIX array detection boosts resolution and the signal-to-background-ratio.
Sample by Hui Chen, PhD. University of South Carolina, Columbia, SC, USA
#superresolution #STEDmicroscopy
A cilium of a fixed RPE-1 cell, expanded and imaged with #3DSTED and #FLEXPOSURE adaptive illumination. CEP164 🟥 (STAR RED), acetylated tubulin+CCDC41 🟦 (STAR ORANGE).
Samples courtesy: Dong Kong & Jadranka Loncarek. Cancer Innovation Laboratory, NCI/CCR.
#ExM #superresolution #fluorescencefriday
#fluorescencefriday with centriolar rosettes in fixed RPE-1 cells, imaged confocal / with #3DSTED and #FLEXPOSURE adaptive illumination.
Labeling: CP110🟥(STAR RED), SAS-6🟦(STAR ORANGE).
Sample courtesy: Catherine Sullenberger & Jadranka Loncarek, Cancer Innovation Lab, NCI/CCR.
#superresolution
#HIV cracks the #nuclearporecomplex during passage😲
👉abberior #3DSTED was used to show that HIV capsids accumulate at nuclear pores of the host cell
👉abberior #STED was used to determine the exact localization of HIV capsid relative to the nuclear pore
😎
#superresolution #STEDmicroscopy #virology
