Effective Agrobacterium-mediated genetic transformation of #okra (#Abelmoschus esculentus) and generation of #RNAi plants resistant to #Begomovirus.
To rediscover one of our most read articles in JHSB 99:2, visit: doi.org/10.1080/1462...
#Agrobacterium
Ground breaking new article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#PlantandEnvironmentalScience
#CRISPR–Cas
#Nuclease
#TransgenicLine
#GuideRNA
#gRNA
#Agrobacterium
Fig.1c
Ground breaking new article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#PlantandEnvironmentalScience
#CRISPR–Cas
#Nuclease
#TransgenicLine
#GuideRNA
#gRNA
#Agrobacterium
Fig.1a
FIGURE 1 County and trial locations of walnut rootstock trials.
J. Hasey et al. report on orchard evaluations of resistance to #Agrobacterium tumefaciens in experimental clonal walnut rootstocks. Learn more: https://doi.org/10.1094/PHP-09-24-0091-RS
RUBY marker revealed transformation events in floral organs and female tissues.
Optimization for #Arabidopsis #floral #dip transformation
📖 nph.onlinelibrary.wiley.com/doi/10.1111/...
#Methods article by Liu et al.
@erhminlai @WileyPlantSci #PlantScience #Agrobacterium
Fig. 2. Presence and absence of bacterial genes that influence plant hormone production or are known virulence factors in pathogenic Agrobacterium fabrum. Bacterial isolates are arranged on the vertical axis and genes on the horizontal axis. The legend shows whether a given gene is involved in plant hormone production or virulence.
Nicholas R. LeBlanc et al. characterized a novel endophytic #Agrobacterium sp. El2ro-1b from strawberry by generating and analyzing a high-quality genome assembly and testing the effects of the endophyte on plant growth: https://doi.org/10.1094/PBIOMES-01-25-0003-SC
A coastal perennial Mimulus guttatus plant overexpressing an anthocyanin MYB gene. The leaves and buds, normally green, are a deep purple, and the flower, normally yellow, is a bright red-orange
Just add water: A simple floral bud injection method for stable Agrobacterium-mediated transformation in two ecotypes of #Mimulus guttatus
New in #AppsPlantSci by @lestanley.bsky.social & @davidlowry.bsky.social
bsapubs.onlinelibrary.wiley.com/doi/full/10.... #botany #Agrobacterium #plantscience
Thanks for publishing in #AppsPlantSci, @lestanley.bsky.social & @davidlowry.bsky.social!
bsapubs.onlinelibrary.wiley.com/doi/full/10.... #Agrobacterium #botany #plantscience
Double-header @IPMBSinica agrofest seminars today @TheSainsburyLab with Erh-Min @erhminlai and Chih-hang @AdamWu9527 🇹🇼 #Agrobacterium #agrobacteria
Great pleasure hosting @fuquaclay.bsky.social visiting IPMB, Academia Sinica. Enjoy scientific discussion, casual chatting over delicious meals and outing. #Agrobacterium #Taiwan
New disarmed hypervirulent Agrobacterium strains + CRISPR tools boost plant transformation! Revolutionizing biotech workflows. #Agrobacterium #SynBio #PlantBiotech
Details: doi.org/10.1016/j.bidere.2025.100001
Happy to write this News & Views highlighting a groundbreaking study on #Agrobacterium. By combining mathematical modelling with quantitative live-cell imaging, the study uncovered a density-dependent synergistic and antagonistic effect on #AMT
👀 EFR-mediated PTI restricts transient transformation and genome editing, but not T-DNA integration.
This study combines floral stage targeting 🌷, immune evasion 🛡️, and Agrobacterium engineering 🔧 to boost plant genome editing efficiency.
#Agrobacterium
#GenomeEditing
www.biorxiv.org/content/10.1...
TIL: #Agrobacterium_tumefaciens
en.wikipedia.org/wiki/Agrobac...
"Plant genomes can be engineered by use of #Agrobacterium for the delivery of sequences hosted in #TDNABinaryVectors."
Scientists at our #QUT node have found a way to reprogram #Agrobacterium — a naturally occurring #bacteria. This breakthrough could lead to exciting advances in areas such as protein production, environmental monitoring and #agriculture. 🌾 Congratulations to the team! bit.ly/3XN6hD5
The #phytochromes Agp1 and Agp2 of the soil bacterium #Agrobacterium fabrum control #BacterialConjugation and #GeneTransfer to plants.
This study provides insights into the interactions between Agp1 & Agp2
🖊️ Tilman Lamparter & team
➡️ bit.ly/41vhXLB
#Photoreceptors
Fig. 4. Visual confirmation of localization using NLS-bearing mCherry reporters and Cas9 variants, and in vitro cleavage assay of NLS-fused Cas9 variants. (E) Box and whisker plots show the fluorescence ratio (as a localization measure) derived from four biological replicates and quantified using ImageJ software. The median (the center line) ± whiskers (1.5 × the interquartile range of the lower and upper quartiles) are shown. Student’s t test was performed. The fold-changes are presented with the statistical significance. The region of interest for the quantification is indicated in the inset. (F) Expected cut site and lengths of the resulting cleavage fragments when PCR-amplified MAA7 (Cre03.g161400) gene of Chlamydomonas reinhardtii is subjected to in vitro cleavage. cCas9 indicates a commercial Cas9. cPAM: complementary protospacer adjacent motif; U.D.: uncleaved DNA (i.e., intact PCR product); and C.F.: cleavage fragment.
Fig. 5. In vivo application of Cas9 variants for algal mutagenesis and investigation of the genetic variance as a result of NHEJ. (A) Representative plate images obtained from the mutagenesis of Chlamydomonas reinhardtii by each Cas9 variant. (B) Simplified description of a strain selection strategy based on tryptophan auxotrophy, when the MAA7 gene is disrupted. The synthesis of the proteinogenic amino acid, L-Trp, from intracellularly synthesized indole is impaired by ΔMAA7. It also deprives cells of the ability to synthesize the cytotoxic, nonproteinogenic amino acid, 5-Fluoro- L- Trp, which is produced when 5-fluoroindole is supplied extracellularly. (C) Mutagenesis frequencies from two scoring methods, namely the number of colony formations and short-read deep sequencing (SR deep seq.), are compared. The absence and presence of L-Trp and 5-FI in each assay are indicated below two vertical axes. The mutation frequencies from the SR deep seq. were corrected with the error rate from the WT sample (SI Appendix, Table S2) and are presented as bars. The individual colony count data are shown as dots with biological triplicates. Floating bars represent the mean. Student’s t test was performed on the frequencies from the colony counts. The fold-changes are presented together with the statistical significance. The mutagenesis frequency is further calibrated with the mutagenesis efficiency calculated from the Sanger sequencing results (Table 2).
Fig. 6. Validation of cross-species versatility using industrial microalga, Chlorella Sp. HS2. (A) The three-dimensional structure of the putative Impα (Hscell_00006266) in Chlorella Sp. HS2 was predicted using AlphaFold. The structural homology provided a rationale for applying the NLS-mediated delivery enhancement strategy. Armadillo repeat motifs (Arms) in the predicted structure are presented. N and C termini are indicated. A microscopic image of Chlorella Sp. HS2 is shown at the Upper Right. (Scale bar, 3 μm.) (B) Representative plate images (from replicate 1 in SI Appendix, Table S3) obtained from the mutagenesis of Chlorella Sp. HS2 targeting the MAA7 gene by each Cas9 variant. (C) Mutagenesis frequencies from the targeted mutagenesis frequency derived from colony formation followed by Sanger sequencing and SR deep seq. are compared. The absence and presence of L-Trp and 5-FI in each assay are indicated below two vertical axes. The mutation frequencies from the SR deep seq. were corrected with the error rate from the WT sample (SI Appendix, Table S4) and are presented as bars. The individual targeted mutagenesis frequency data are shown as dots with technical triplicates. Floating bars represent the mean. Student’s t test was performed on the targeted mutagenesis frequencies. The fold-changes are presented together with the statistical significance.
Great work by Le, Choi, Kim et al. (2025) on how the functional fusion of #Agrobacterium VirD2-derived nuclear localization signal (NLS) to #Cas9 improved its nuclear import, thereby doubling the gene-editing frequency in #algae, #Chlamydomonas and Chlorella Sp. HS2.
🔗 www.pnas.org/doi/10.1073/...
#Agrobacterium 2025. The 45th American Crown Gall meeting and the 5th European Agrobacterium conference: registration and abstract submission is now open! #deadline May 1, 2025. Thanks to our sponsors we will select some students for travel grants.
www.kuleuven.be/agrobacterium2025
Coming Soon!
Samatha Gunapati and Aimee Malzahn interviewed Keunsub Lee about his work with Agrobacterium strains and his role as a Steering Committee member for PlantGENE.
Stay tuned for the full episode!
#ScienceCommunication #Agrobacterium #Biotechnology
Save the date! #AGROBACTERIUM2025 Joint event of the 45st American Crown Gall meeting and the 5th European #Agrobacterium conference SEPTEMBER 15-16 2025, LEUVEN, BELGIUM More info soon!
Who is Pepe? What is the relationship between transgenic food and Pokémon world? What butterfly effect am I talking about?
Discover it in the new Biostorytime video, now available on YouTube
youtu.be/Zn2ND8XRZOE?...
#biotechnology #transgenicfood #geneticedition #agrobacterium
¿Quién es Pepe? ¿Qué relación tienen los alimentos transgénicos con el mundo Pokémon? ¿De qué efecto mariposa estoy hablando?
Descúbrelo en el nuevo vídeo de Biostorytime, ya disponible en Youtube
youtu.be/Zn2ND8XRZOE?...
#biotecnologia #alimentostransgenicos #ediciongenica #agrobacterium
#CRISPR is revolutionizing plant breeding. However, plant transformation remains an obstacle. The 1st MoBi group we introduce is Plant Transformation & Gene Editing @laurenspauwels.bsky.social studying the processes behind #Agrobacterium mediated transformation & plant regeneration. #plantscience
First post on @bsky.app highlighting our collaboration with Ana Caño lab (CRAG) on on using BBM/WUS-technology and a ternary vector system to transform #sorghum and maize. Now published in @theplantjournal.bsky.social @psb-vib.bsky.social #Agrobacterium
📜 Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation
🧑🔬 Davide Annese, @jimhaseloff.bsky.social, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ #PlantScience #PlantTransformation #Agrobacterium #PlantMethods
📜 Genomic consequences associated with Agrobacterium- mediated transformation of plants
🧑🔬 Geoffrey Thomson, Lauren Dickinson, Yannick Jacob
📔 The Plant Journal
🔗 onlinelibrary.wiley.com/doi/10.1111/...
#️⃣ #PlantScience #PlantTransformation #Agrobacterium #PlantGMO #PlantGenomics
