- any input appreciated as they continue to work on new landing sites and applications! More to come...
#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning
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#AlwaysBeCloning
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Early for #FluorescenceFriday: make more glowing #zebrafish to observe how beautiful #devbio is!
(dorsal view of a zebrafish embryo, drl:mCherry (magenta) and mixl1(EFM):mCerulean; pic credit Harrison Wells)
@cudevbio.bsky.social #AlwaysBeCloning
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The best.
#AlwaysBeCloning
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Many of you never had to painfully hand-annotate all the EcoRI sites on several printed pages of DNA sequence and it shows.
#PILife #molbio #AlwaysBeCloning
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A well-worn, well-used New England Biolabs catalog from 2002-2003, containing numerous resources for molecular cloning, enzymatic DNA digests, and more.
Resources that never grow old, like:
#AlwaysBeCloning #MakeMoreTransgenics #molbio #RestrictionEnzymesStillMatter 🧪🧫🧬
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Someone, somewhere, needs a pic of a pIGLET-based transgenic #zebrafish today.
(pic credit Seth Jacobson, p14a.ubi:gm130-tdTomato) 🐽🍅
#MakeMoreTransgenics #AlwaysBeCloning #devbio
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#LabLife, pt. 2
(credit to Emily Peden @cudevbio.bsky.social)
#AlwaysBeCloning 🧬
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One of our lab's trusty PCR machines @cudevbio.bsky.social, affectionately called Kermit, got a bit of...bling today:
#PILife #devbio #AlwaysBeCloning 🧬🐟
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"Till you have sequence confirmation, you are not cloning but clowning." 🧬⭕🤡
- Lab Saying.
#PILife #AlwaysBeCloning
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Transgenic zebrafish embryo with mpeg1.1:GFP in green and a new enhancer reporter from the meis2b gene expressing mApple in magenta.
#FluorescenceFriday, or when the enhancer you're testing is not doing what you expect (or understand!).
-3.4meis2b:mApple (magenta) with mpeg1.1:GFP; pic cred Hannah Moran.
Happy to share the line if anyone's interested, otherwise...🪦
#zebrafish #devbio #AlwaysBeCloning #MakeMoreTransgenics
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