- any input appreciated as they continue to work on new landing sites and applications! More to come...

#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning

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Looking forward to when we can ask such questions for #zebrafish!
#MakeMoreTransgenics #ShareYourTools

A collection of goodies from the Young Researcher Committee of the Europeanl Zebrafish Society: a coffee mug with a zebrafish and DNA art drawing, a tote bag with the committee's logo, and a thank-you note.

Dlighted to receive these from the YoRC❤️, following the early-career workshop at the @izfs.bsky.social meeting in Madison - feat. Zeeks/Elisabeth Kugler's mug design!
#zebrafish #collaborate #ShareYourTools #ConferenceSummer #PILife

piglet from winnie the pooh says thank you ALT: piglet from winnie the pooh says thank you

Grateful to receive an R24 NOA from NIH/ORIP to advance phiC31-based transgenesis tools in #zebrafish. 🐟

Collab with @aburger2009.bsky.social & Breuss labs @cuanschutz.bsky.social. 🧬

...and: big THX to everyone who already helped w/ sharing #pIGLET reagents! 🐽
#ShareYourTools #MakeMoreTransgenics

Coming soon to a webinar format in your vicinity....more tk!
#zebrafish #devbio #ShareYourTools 🐽

Congrats all.

Should really still cite "commonly accessible" vector sources, like Kwan et al. 2007. Are the supplemental tables with the cloning details available?
#ShareYourTools #zebrafish

Achievement unlocked:
this simple box flew from CU Anschutz🌎 > University of Otago🌏, after months of paperwork & coordination.

...and now, the #zebrafish pIGLET landing site lines are in New Zealand! Looking forward to the new transgenics from the NZ community.
#ShareYourTools #MakeMoreTransgenics

50 ml Falcon tubes with zebrafish embryos and a Whatman filter of plasmids for the pIGLET site-directed transgenesis system using phiC31 integrase, shared with the Dennis lab at UCDavis.

Some small wins: this week's pIGLET shipments arrived at their new homes!

Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟

(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools

We created a suite of attB backbone vectors (Multisite Gateway, restriction sites) with and without transgenesis markers, primer info for quality control, & detailed protocols.

All vectors are being deposited with Addgene and available from us! #ShareYourTools

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