- any input appreciated as they continue to work on new landing sites and applications! More to come...

#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning

(6/6)

#FluorescenceFriday with a transgenic zebrafish labeling descendant lineages of the lateral plate mesoderm.

(Ventral view, tmem88a:EGFP, pic cred Marycruz Flores Flores @cudevbio.bsky.social). ❤️🫀🐟🧬
#devbio #cardiacdevbio #zebrafish #MakeMoreTransgenics

Good memories!
#devbio #zebrafish #zerbafish #MakeMoreTransgenics

Looking forward to when we can ask such questions for #zebrafish!
#MakeMoreTransgenics #ShareYourTools

A well-worn, well-used New England Biolabs catalog from 2002-2003, containing numerous resources for molecular cloning, enzymatic DNA digests, and more.

Resources that never grow old, like:
#AlwaysBeCloning #MakeMoreTransgenics #molbio #RestrictionEnzymesStillMatter 🧪🧫🧬

piglet from winnie the pooh says thank you ALT: piglet from winnie the pooh says thank you

Grateful to receive an R24 NOA from NIH/ORIP to advance phiC31-based transgenesis tools in #zebrafish. 🐟

Collab with @aburger2009.bsky.social & Breuss labs @cuanschutz.bsky.social. 🧬

...and: big THX to everyone who already helped w/ sharing #pIGLET reagents! 🐽
#ShareYourTools #MakeMoreTransgenics

It's SPIM-ing time again! @mary-flores.bsky.social leading the charge to image new reporters with Amanda Garfield & Emma Harrison. @cudevbio.bsky.social #zebrafish #MakeMoreTransgenics #Imaging #devbio

Someone, somewhere, needs a pic of a pIGLET-based transgenic #zebrafish today.

(pic credit Seth Jacobson, p14a.ubi:gm130-tdTomato) 🐽🍅
#MakeMoreTransgenics #AlwaysBeCloning #devbio

/ rant on:
Abbreviating cis-regulatory elements as *CRE* is a terrible abbreviation in a field that uses *Cre* recombinase as major lineage trace tool. 🐭🐟🧬
/ rant off

#devbio #MakeMoreTransgenics #Crelox #enhancers

Achievement unlocked:
this simple box flew from CU Anschutz🌎 > University of Otago🌏, after months of paperwork & coordination.

...and now, the #zebrafish pIGLET landing site lines are in New Zealand! Looking forward to the new transgenics from the NZ community.
#ShareYourTools #MakeMoreTransgenics

90+ labs got pIGLET lines & reagents now. 🌎🌍🌏
First labs report targeted germline integrations & beautiful new transgenics - we are always keen to hear your updates!

Ping us about trouble with genotyping, cloning, etc. - email or Slack @zebrafishrock.bsky.social. 🐽
#MakeMoreTransgenics #zebrafish

50 ml Falcon tubes with zebrafish embryos and a Whatman filter of plasmids for the pIGLET site-directed transgenesis system using phiC31 integrase, shared with the Dennis lab at UCDavis.

Some small wins: this week's pIGLET shipments arrived at their new homes!

Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟

(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools

First zoom with new collaborator and all involved trainees =
- inspired buzzing in the lab
- puts faces to names from prior emails
- motivating common goal

...but importantly
- smiles all around!

#PILife #CHD #devbio #collaborate #GoFish #MakeMoreTransgenics 🐟🧬🧫🫀

1st pIGLET lines of 2025 coming @cudevbio.bsky.social - today:
pIGLET14-based hsp70l-driven loxP transgene with tdTomato-fused reporter + exorh:EGFP marker; tested with drl:creERT2, heat-shocked/imaged at 4 dpf.

Congrats to Amanda Garfield on her first transgenic! 🐽
#zebrafish #MakeMoreTransgenics

Lots to learn and to dig into with these new drivers - congrats all!
#collaborate #MakeMoreTransgenics

Transgenic zebrafish embryo during segmentation stages, brightfield outline together with green fluorescent channel showing the drl:EGFP trangene that labels the lateral plate mesoderm.

Lateral plate to brighten the day ❤️⚾.
(drl:EGFP; pic cred Harrison Wells)

#zebrafish #devbio #MakeMoreTransgenics

Ours to compare (pic from 2021 but unchanged except the fall decoration):
every transgenic or mutant ever from our lab was done with needles from this beauty. First at UZH, now @cuanschutz.bsky.social.
#zebrafish #MakeMoreTransgenics

Transgenic zebrafish embryo with mpeg1.1:GFP in green and a new enhancer reporter from the meis2b gene expressing mApple in magenta.

#FluorescenceFriday, or when the enhancer you're testing is not doing what you expect (or understand!).

-3.4meis2b:mApple (magenta) with mpeg1.1:GFP; pic cred Hannah Moran.

Happy to share the line if anyone's interested, otherwise...🪦
#zebrafish #devbio #AlwaysBeCloning #MakeMoreTransgenics