Figure 2: Key practical parameters to consider for live-cell DNA imaging.

(3/6) The 'How-To' Guide. We provide a practical checklist for designing an imaging experiment, covering critical factors such as SNR vs SBR, optimizing probe design & delivery, spatial–temporal resolution, and microscope choices. #LiveCellImaging #Microscopy #SNR #Resolution #Biophysics

1/3 GPU algorithms transform consumer graphics cards into molecular microscopes—protein folding simulations now achieve millisecond resolution on desktop computers, revealing mechanical properties of cytoskeletal structures. #academicsky #biophysics

A new study in The FEBS Journal reveals how the "crowded" interior of a cell dictates DNA triplex stability.

Illustrations made using #ProteinImager

Full study here: doi.org/10.1111/febs...

#DNA #Biophysics #Genomics #MolecularBiology #Research #CellBiology #Genetics

#Suppressed #Science

Besides obvious contrast between Tesla's #ether #physics & Reich's #orgone #biophysics, another difference between William Lyne's & James DeMeo's #published #research is in the № of #exposed #spies still alive.
Both authors name names, but Lyne focused on 19th & 20th Centuries.

自己組織化する細菌集団の潜在的行動パターン：深層学習による時空間ダイナミクスの解析 AI解析で細菌の集団行動における隠れた時空間パターンを解明

自己組織化する細菌集団の潜在的行動パターン：深層学習による時空間ダイナミクスの解析

AI解析で細菌の集団行動における隠れた時空間パターンを解明

#AI #Biophysics #ComputerVision #PatternRecognition #ComplexityScience

Myxococcus xanthusの自己組織化を定量化する深層学習フレームワークの技術的解読 細菌の動的な自己組織化プロセスを深層学習で定量解析する新手法の解説。

Myxococcus xanthusの自己組織化を定量化する深層学習フレームワークの技術的解読

細菌の動的な自己組織化プロセスを深層学習で定量解析する新手法の解説。

#DeepLearning #ComputerVision #Biophysics #SelfOrganization #FeatureExtraction

In this new paper from #JRSocInterface, researchers created a stochastic, mechanistic model to study how neoantigens drive T-cell expansion in the tumor microenvironment of brain tumors doi.org/10.1098/rsif... | #Biophysics #ComputationalBiology #SystemsBiology

CRISPR–Cas-based live cell imaging of genome dynamics - Nature Reviews Genetics In this Review, Zhu et al. outline the various CRISPR-based tools recently built for dynamic DNA and RNA imaging, which have provided insights into various molecular mechanisms. The authors also discuss the important parameters to consider when using these tools, and how to approach the quantitative image analysis.

From cancer dysfunction to T cell exhaustion, the "live" 3D genome holds the answers.

Read our definitive reference in
@NaturePortfolio
: nature.com/articles/s41...

#CRISPR #Genomics #Biophysics #Stanford #3DGenomics #ScienceTwitter

(5/5)

🔗 Quantum Biology Using Ultrafast Integrative and Molecular Engineering Tools: A Perspective. Computational and Structural Biotechnology Journal (CSBJ). DOI: https://doi.org/10.34133/csbj.0024 📚 CSBJ Quantum Biology and Biophotonics: https://spj.science.org/journal/csbj/qbio

🔗 Quantum Biology Using Ultrafast Integrative and Molecular Engineering Tools: A Perspective. DOI: doi.org/10.34133/csb...

📚 CSBJ Quantum Biology and Biophotonics: spj.science.org/journal/csbj...

#QuantumBiology #QuantumPhysics #MolecularBiology #ProteinEngineering #Biophysics #Biochemistry #Life

New research from #JRSocInterface: First-nearest neighbour interactions and Vicsek-type noise in shoals of Paracheirodon axelrodi fish doi.org/10.1098/rsif... | #Biophysics #Tetra

New book edited by Prof. Irena Roterman-Konieczna (Sano ISC) “Simulating Protein Folding in Variable Environmental Conditions” (Elsevier, 2026)
Learn more: shop.elsevier.com/books/simula... #ProteinFolding #Amyloids #ComputationalBiology #StructuralBiology #Biophysics #ProteinScience

Beyond a Passive Tether: Structural Insights into the Disordered Tail of Hsp90 The molecular chaperone Hsp90 is an abundant and essential homodimer that supports the stability and folding of hundreds of client proteins in cells. Its C-terminal domain (CTD) mediates dimerization and serves as a docking site for cochaperones bearing tetratricopeptide repeat (TPR) domains, which recognize the conserved MEEVD motif located at the end of an intrinsically disordered CTD tail. Despite its conservation, the structural role of this tail remains poorly understood. We investigated the conformational behavior of the yeast Hsp90 (yHsp90) CTD tail and its response to the binding of the TPR-containing PPIase cochaperone Cpr6. Using site-directed spin labeling with nitroxide and Gd(III) labels, we examined full-length yHsp90 (FL), isolated CTD (IsoC), and tail-truncated variants by double electron–electron resonance (DEER) and EPR spectroscopy. The CTD tails in IsoC and FL adopted distinct conformational ensembles, attributed to intramolecular interactions with the middle domain in FL. Cpr6 binding abolished these differences, indicating disruption of intramolecular tail contacts. In IsoC, the tail also stabilized an additional CTD conformation near the dimer interface that was absent in FL and was lost upon tail truncation, consistent with tail–CTD interactions observed by NMR. This population was reduced upon Cpr6 binding, shifting the IsoC conformational distribution toward that of FL. Additionally, this population was reduced in cellular environments and mimics. Together, our results demonstrate that the disordered CTD tail is an active structural element that modulates both its own conformational ensemble and CTD architecture, highlighting its potential functional relevance in the Hsp90 chaperone cycle.

✨ New paper out in #JACS! We looked at the intrinsically disordered CTD tail of #Hsp90 often seen as a passive linker. Turns out, it’s not.
#StructuralBiology #Biophysics #EPR #NMR
pubs.acs.org/doi/10.1021/...

Join the NITheCS Colloquium: ‘Modelling deformation in biological cells’, presented by Dr Rhoda Hawkins (AIMS Ghana & University of Sheffield).
🗓 Mon, 13 Apr
⏰ 16h00–17h00 SAST
📍 Attend in person or online
buff.ly/3tq7rKE
#Biophysics #CellBiology #ComputationalBiology #SoftMatter #BiologicalPhysics

1/5
🤔 What can you actually do with #FLUCS?
💡 FLUCS enables researchers to actively & flexibly control intracellular flows. Non-invasively & label-free.
📩 Get in contact with us to explore how FLUCS can add a new dimension to your research.
#Photomanipulation #Bioimaging #Biophysics

Lipid Research Division Seminar Series April 9, 2026 | 12 p.m. Eastern

Upcoming #lipid webinar tomorrow: www.asbmb.org/meetings-eve...

Zak Baker (WashU) and Sara Wong (Utah) on mitochondrial lipids, including the role of unsaturated lipids. Sounds interesting! #PUFA #biophysics #mitochondria

Register at the link above.

1/3 Living cells 'glow' with distinct microwave signatures at 2.4 GHz—revealing their inner chemistry without dyes or needles. #academicsky #biophysics

Fluid flow–induced biomechanical origin of collagen architecture in articular cartilage royalsocietypublishing.org/rsif/article... | #JRSocInterface #Bioengineering #Biomechanics #Biophysics

1/3 Microtubules maintain 1-millimeter persistence lengths to prevent cargo jams that trigger neuron death during axonal transport. #academicsky #biophysics

🧪 Just out: Issue 3 of ABBS
🔬 uncovers:
🧠 MOTS-c peptide against cancer-induced bone pain
🩺 Pancreatic sympathetic innervation diabetes
🌱 3D ovary culture model with quercetin
… and more
www.sciengine.com/ABBS/issue

#Biochemistry #Biophysics #CellBiology #OpenAccess #AcademicTwitter #BlueskyScience

1/3 35,000 annual simulations track nanosecond shape changes that prevent protein misfolding deaths in neurons. #academicsky #biophysics

Gaussian closure and dynamical mean-field theory for self-avoiding heteropolymers Analytical treatments of polymer dynamics have mostly been restricted to linear response theory around some steady state obtained via perturbative field theory. Here, I derive an analytical framework ...

How do active processes affect polymer conformations? I first began studying this question using linear response theory, and long thought that more general models could only be tackled with simulations. Turns out I was wrong: doi.org/10.48550/arX.... (1/8)
#active #chromatin #folding #biophysics

1/4 Tubulin proteins deform within 10 nanoseconds of intense electric field exposure, shifting conformation by sub-nanometer distances to alter cellular signaling dynamics. #academicsky #biophysics

Externally driven condensates show translation-induced polarization, directed coalescence, and anomalous diffusion in viscoelastic media Phase separation into compositionally and physically distinct domains is ubiquitous in (non)living matter ranging from alloys and emulsions to biomolecular condensates in cells. The organization of th...

Do different regions in the cell nucleus find each other by random motion or is there a directed component? In short: likely both. Below, I summarize a few predictions from a recently updated preprint from last June: doi.org/10.48550/arX.... (1/9)
#biophysics #theory #active #chromatin #condensates

Over the course of two years (1937–1939), #experiment after experiment confirmed
the #vesicular #disintegration of #swelling #matter and the #organization of #bacteria and #cells from the #bions.
Wilhelm Reich
The #Cancer Biopathy

#suppressed #orgone #biophysics #history
#biosky #psychsky #oncology

"The substances were mixed and boiled, and my colleagues detected cell-like moving structures immediately after boiling."

Wilhelm Reich to Roger du Teil
12 December 1936
Beyond Psychology p.81
Edited by Mary Boyd Higgins

#orgone #biophysics #history
#biosky #medsky #psychsky
#dystopian #war #crime

One method to rule them all - comprehensive evaluatioin of desiging #asymmetric #membranes using #invertedemulsion method. This high potential method can jump start the #biophysics in a whole new direction. #synhteticcells

1/4 Microtubules should vibrate at radio frequencies up to 100 MHz according to models, but cellular viscosity damps these motions 1000-fold below detection limits. #academicsky #biophysics

Sign up now for the EMBO Workshop "#MolecularBiology of #Archaea: Life Through the Prism of Archaea" in Cambridge, UK, 6–10 July 2026.

Deadline: 15 April

https://meetings.embo.org/event/26-archaea
#EMBOArchaeaBiology #EMBOevents #CellBiology #StructuralBiology #ecology #evolution #biophysics 🧪

1/3 Microtubules in cellular fluid exhibit overdamped bending motions that decay exponentially, unlike the periodic oscillations seen in air. The surrounding cytosol fundamentally alters their mechanical behavior. #academicsky #microtubules #biophysics

3/3 The implications are striking: non-thermal EM field effects on oxidative stress management may be physically plausible through direct enzyme modulation. #academicsky #biophysics