The #ChlamyDataset has now been used to elucidate the protein extensions of the #chloroplast #ribosome 💚

The #ChlamyDataset is on the cover of @cp-molcell.bsky.social 🖼️🥰!

Read @lifeonthewedge.bsky.social's great thread🧵 for the inside scoop🍨 on all the #TeamTomo developments already made possible since these 1829 tomos hit EMPIAR 🧪 🧶🧬

For more, here's the old preprint thread:
bsky.app/profile/cell...

This is less than 2 years after releasing the dataset and 1 year after the #preprint – and I'm sure more stuff is on the way. Apologies if I have missed some method or application based on the #ChlamyDataset – please let me know, will be happy to include it in the thread!

PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms Cryo-electron tomography (cryo-ET) datasets are rich sources of information capable of describing the localizations, structures, and interactions of macromolecules. However, most current methods for l...

#PickET is a pretty cool semantic segmentation and particle picker tool that was also demonstrated on the #ChlamyDataset:

🍦IceCream, a denoising and missing wedge estimation tool, has also relied a lot on the #ChlamyDataset for development:

For a starter, #MemBrain-seg from @lorenzlamm.bsky.social would not have been possible without the #ChlamyDataset and lots of other kind contributions from the #CryoET community 🙏🏽

#TeamTomo

The #ChlamyDataset is intended to be a public resource for methods development and scientific discovery, and I'm happy to say it has been adopted very early by the #CryoET community, even before the preprint was out. So below I’ll highlight some of the cool stuff that have been done with it 🤗

This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell!

This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍

[Maybe long thread ahead]

EMPIAR-11830 #ChlamyDataset #TeamTomo 🧪

www.cell.com/molecular-ce...

Happy to see IsoNet2 performing so well on the #ChlamyDataset EMPIAR-11830. Congrats to the authors really great work.
However, I have to say this tomogram looks amazing because the lamella is way too thin (~50 nm) and not vitreous! 🫠

www.biorxiv.org/content/10.6...

Always happy to see the #ChlamyDataset put to good use 😉

Thanks Japan for using the #ChlamyDataset

Looking forward to this meeting! 🤓💻🧬🦠🔬

#ChlamyDataset will be there too 💚

My colleagues @phaips.vd.st and @florentwaltz.bsky.social have put together this wonderful website with step-by-step tutorials for in situ subtomogram averaging using different software packages 🤩

Check it out, especially if you are a newcomer to the field!

#CryoET #TeamTomo #ChlamyDataset

ChlamyAnnotations/chlamydataset2relion5 at master · Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations Particle annotations for the large-scale cryo-ET dataset of Chlamydomonas reinhardtii - Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations

To all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging:

github.com/Chromatin-St...

#Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience