Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature Cas9 structures explain topology sensing and off-target activation.

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵

🔗 www.nature.com/articles/s41...

#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

The R-loop grammar predicts R-loop formation under different topological constraints Author summary R-loops are transient three-stranded helical structures that form when newly synthesized RNA binds back to the DNA template strand during transcription, displacing the non-template stra...

Our new paper in PLOS CompBio introduces the R-loop grammar—a formal-language model that predicts R-loop formation across different DNA topologies from SMRF-seq data alone. It captures topology outperforming other methods
journals.plos.org/ploscompbiol...
#ComputationalBiology #Rloops #DNAtopology

Submolecular video-imaging of the Smc5/6 complex topologically bound to DNA
Kodera, N., Kurokawa, Y. et al.
Paper
Details
#SubmolecularImaging #Smc56Complex #DNATopology

Lynn Zechiedrich BCM

We are pleased to welcome Professor Lynn Zechiedrich (Baylor College of Medicine), a leading expert in DNA topology, supercoiling & topoisomerases, to our JBSD Editorial Board. #JBSD #DNATopology #Topoisomerase #NucleicAcids www.bcm.edu/people-searc...