Fig. 2. The percentage of hop cones (n = 10 per week) that had Diaporthe humulicola detected using two different methods, plating of cone tissue onto potato dextrose agar (solid line) and a translation elongation factor 1-alpha–based TaqMan quantitative PCR assay that was run on DNA extracted from the cones used for isolation (dotted line). Cones were collected from the same plant in a research yard in East Lansing, Michigan, over 10 weeks (starting 22 May) in 2022.
The (TEF)-based TaqMan qPCR assay developed by Ross J. Hatlen et al. provides time-limited diagnosticians with a tool for the detection of #Diaporthe humulicola, the causal agent of halo blight of hop. Learn more: https://doi.org/10.1094/PDIS-11-24-2331-SR