Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replication - Nature Communications Cheng et al., use non-infectious stable viral replicon cell lines in genome-wide CRISPR KO screens across diverse viral pathogens to identify host factors specifically involved in the intracellular stages of viral replication.

New paper introduces a virus replicon–based genome-wide #CRISPR KO screening platform that identifies #HostFactors essential for replication of diverse RNA viruses, from dengue to chikungunya and Ebola. #Virology
📄 https://doi.org/10.1038/s41467-025-65979-3
👤 EVBC member: Jens H. Kuhn

New evidence shows SLBP enhances SARS-CoV-2 replication by promoting –1 ribosomal frameshifting.
#SARSCoV2 #Frameshifting #ViralReplication #HostFactors #CoronavirusResearch
www.nature.com/articles/s41...