The figure shows the fibrosis analysis. Top panel shows images of diaphragm, quadriceps and tibialis anterior sections from mice stained with Picro Sirius Red, which reveal collagen deposition as an indicator of fibrosis. Bottom panel shows violin plots representing the quantification of the percentage area of fibrosis in the diaphragm, quadriceps or tibialis anterior of wild-type, heterozygous and homozygous mice aged 2, 6 or 12 months.
Arístides López-Márquez, Cecilia Jiménez-Mallebrera & co used CRISPR/Cas9 to generate the first #MouseModel of a pathogenic variant that involves glycine substitutions in the triple helical domain of collagen VI alpha chains and leads to congenital #MuscleDisease.
Read doi.org/10.1242/dmm....
