Optimise in vitro transcription reactions with high yield T7 mRNA #polymerases and #synthesis kits from #JenaBioscience GmbH, available with varied combinations of Cap moiety & #nucleotide modifications for optimal translation and minimal immunogenicity
🔗 www.stratech.co.uk/jena_bioscie...

Researchers identify subtype-specific 20–40 bp #nucleotide fragments across #InfluenzaA genomes, enabling high-accuracy detection and HA-based subtyping, and demonstrate that M- and HA-derived #primers can support molecular surveillance.
#OpenAccess: doi.org/10.1016/j.dc...

Identification of a Novel Linker Enabling the Bioconjugation of a Cyclic Dinucleotide for the STING Antibody-Drug Conjugate TAK-500 STING activates the innate immune system by inducing type-1 interferon (IFN) production and has been pursued as a therapeutic option in immuno-oncology. The targeted delivery of STING agonists to CCR2+ immune cells could enhance the therapeutic window of the agonists by selectively activating the STING pathway within targeted immune cells. The chemistry strategy was established to enable the targeted delivery of the cyclic dinucleotide STING agonist dazostinag to CCR2+ cells through an antibody-drug conjugate (ADC) approach. A self-immolative spacer between the adenine of dazostinag and the Cathepsin-B cleavable Val-Ala dipeptide linker rendered a linker payload that exhibits strong plasma stability while allowing the rapid payload release upon internalization into lysosomes. The stochastic cysteine conjugation of the dazostinag containing these linkers provided ADC TAK-500 and its mouse surrogate mTAK-500 with DAR = 4. In syngeneic tumor-bearing mouse models, mTAK-500 showed target specific antitumor activity as well as the induction of immune-stimulating cytokines.

Identification of a Novel Linker Enabling the Bioconjugation of a Cyclic Dinucleotide for the STING Antibody-Drug Conjugate TAK-500 | Bioconjugate Chemistry #chemistry #nucleotide #drugdevelopment 🧪
pubs.acs.org/doi/10.1021/...

Digital nucleotide.
#AiArtCommunity #digitalart #AiArt #aidigitalart #nucleotide #matrix #circuits #remixratpack #surrealart #3D #design #INSTAGRAM #classic #cool #portrait #openai #AiCommunity #AiArtChallenge #SynthArt #CreativeAi #Ai #AiArtwork

This includes reprogramming the host’s nucleotide synthesis pathways to fuel viral genome replication & the production of new viral particles.
In this study present a comprehensive analysis of #nucleotide regulation by upstream growth factor signaling mechanisms

Optimise #invitro transcription reactions with high yield T7 mRNA polymerases & synthesis kits from @JenaBioscience

Available with varied combinations of Cap moiety & #nucleotide modifications for optimal translation & minimal immunogenicity
🔗 https://tinyurl.com/48jf8372

#biotech #lifesciences

How to Improve Your RNA-Seq Data with UMIs and ERCC RNA Scientists have developed two powerful tools for RNA sequencing (RNA-Seq) experiments to minimize bias and variability: unique molecular identifiers (UMIs) and ERCC RNA spike-ins. We’ll cover the basi...

What are #UMIs?

UMIs are short, random #nucleotide sequences added to individual #transcripts during library preparation and uniquely identify #cDNA. Learn how this powerful tool cleans up #RNASeq data for #GeneExpression analysis, resulting in improved #RNA-Seq data.

Read now: hubs.ly/Q03nSq1g0

🚨 New preprint available! Third and final in the #CRISPR screen series!🚨

👉🏻 Uridine-sensitized screening identifies genes and metabolic regulators of #nucleotide synthesis

www.biorxiv.org/content/10.1...

By A. Strefeler in collaboration with Dave Pagliarini and team.

@DIB_UNIL @FBM_UNIL

🧵 1/9

Hard to recall what I learned in HS biology. But I like that I can spell and pronounce #NUCLEOTIDE.

The vasoconstrictor adenosine 5′-tetraphosphate is a danger signal that induces IL-1β - Molecular Medicine Background The endogenous nucleotide adenosine 5′-tetraphosphate (Ap4) is a potent vasoconstrictor. Despite its structural similarity to the danger signal adenosine 5’-triphosphate (ATP), the immunomo...

Our paper is out! Could the #nucleotide adenosine 5′-tetraphosphate (Ap4) promote #inflammation? Ap4 triggers IL-1β release distinct from canonical #inflammasome activators, shedding light on its potential role in #inflammaging. Kudos to Judith, Jonas, and collaborators! 👏 doi.org/10.1186/s100...

An early sketch of how rhythm can be used to understand molecular structures.
#ScienceAndArt #SoundArt #TransferEpistemology #Nucleotide #MolecularMusic

📢We're recruiting📢

Our lab at the University of Lausanne is looking for a postdoctoral associate to study #nucleotide metabolism in physiology and cancer in vivo!

Please share

#uridine #cancermetabolism #immunometabolism

@unil.bsky.social

🔗(PDF): www.jourdainlab.org/wp-content/u...

📢We're recruiting📢

Our lab at the University of Lausanne is looking for a postdoctoral associate to study #nucleotide metabolism in physiology and cancer in vivo!

Please share

#uridine #cancermetabolism #immunometabolism

@unil.bsky.social

🔗(PDF): www.jourdainlab.org/wp-content/u...

🚨 @enasequence.bsky.social! I would like to get the growth curve for the #European #Nucleotide #Archive. Basically replicate this curve. Is there anywhere I can access these data?

Fellow Bioinformaticians, Computational Biologists and other nucleotide sequence geeks. Does anyone have this?

Thanks!

Prof. Yitzhak Tor from UC San Diego discussed his research on emissive #nucleoside surrogates, which facilitate real-time monitoring of nucleoside-, #nucleotide- and #nucleic acid-based transformations at nucleobase resolution. He presented examples of their application in biophysical studies.

In the 2nd keynote talk of DGFI #Vaccine #workshop, G. Hartmann speaks of the immunologist dirty little secret - the #adjuvant (h/t to Charlie Janeway). He overviewed nucleic acid recognition by the #immune system and how modern understanding of #nucleotide #biochemistry 1/2