Token #Zombie of the day, was this huntsmen #spider caught by vines. Perhaps an old #purpureocillium victim. But who knows what microbes now call it home? Well hopefully future me lol

A damaged lantana leaf

When I first started hunting #zombie fungi, a legend in the field, Nigel Hywel Jones, gave me a heap of help & guru tips.One was that if you flip the leaf over & its damaged its not a zombie fungi.Which was true until I noticed #purpureocillium rots leaves. Always exceptions to rules with fungi.

First #entomopathogenic #fungi find today was a very elderly #purpureocillium on a spider. Yes, you always want to find a zombie fungi at its peak, the perfect fruiting.... but the trick is to find examples all along their life cycle, as they never not interesting. Only then can you id the random

I had 5 minutes to find a token zombie before suiting up. I didnt even need 1. And it was a stonker too :D nice huntsman spider host, monstered by #Purpureocillium atypicola. This is the Anamorph. The Teleomorph is a cool brown spike, that usually targets our primitive spiders in their holes.

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#PYOCIN
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#PURPUREOCILLIUM #TAKAMIZUSANENSE
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#GEELY #XINGYUE S
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Diversity is something you learn to love. The more different life all cluttered together, the greater the chance of seeing awesome #zombi #fungi #parasitoids like #purpureocillium

Soil fungus and calcium team up to break down bioplastics faster A common soil fungus (Purpureocillium lilacinum BA1S), when combined with calcium and mild alkalinity, speeds up the breakdown of biodegradable plastic (PBAT), offering a greener path for managing…

#Soil #fungus and calcium team up to break down #bioplastics faster ...

| #fungi | #Purpureocillium | #biodegradable | #packaging | #waste | Via @sciencex.bsky.social

Genomic analyses of Purpureocillium lilacinum reveal unique DNA regions for developing isolate-specific molecular markers - BMC Genomics Background The Purpureocillium lilacinum NCHU-NPUST-175 (Pl-NCHU-NPUST-175) strain has been previously reported as an entomopathogenic fungus (EPF) with potential for controlling Forcipomyia taiwana (little black mosquito). In this study, genome sequencing of Pl-NCHU-NPUST-175 was performed via long-read sequencing (Oxford Nanopore Technologies, ONT). The comparative genomic analysis was conducted to identify isolate- and species-specific DNA regions for developing markers for rapid molecular identification, in order to detect the fungus in an environment and to confirm species identity before practical field applications. Results The genome assembly of Pl-NCHU-NPUST-175 is 36.55 Mb, consisting of 13 contigs with a GC content of 58.64%. The largest contig of the Pl-NCHU-NPUST-175 genome was 6.79 Mb, and the N50 value was 4.08 Mb. The genome contains 14,069 putative protein-coding genes, with a gene density of 384.90 genes per Mb and a median number of three exons per gene. Additionally, one mitogenome, Pl-NCHU-NPUST-175, was assembled with a size of 0.23 Mb and contained 16 protein-coding genes. In the Pl-NCHU-NPUST-175 genome, repeat regions accounted for 3.70% of the genome. Most repetitive sequences exhibit a nucleotide sequence divergence of less than 30%. Phylogenetic analysis revealed that Pl-NCHU-NPUST-175 is most closely related to other P. lilacinum strains. To identify distinctive genomic regions (DGRs), a comparative genomics analysis was conducted between Pl-NCHU-NPUST-175, P. takamizusanense and P. lilacinum. A total of 1,154 unaligned fragments were identified by genome-wide alignment, ten DGRs were randomly selected for specific primer design, and the specificity of the primer sets was tested. The results showed that all primer sets could serve as specific molecular markers for Pl-NCHU-NPUST-175. Among the ten primer sets, three primer sets were selected for the soil-sample detection test. The qualitative PCR results indicate that all three isolate-specific primer sets (pl_1, pl_8, and pl_10) detected 105 conidia g−1 soil in the natural soil samples for up to 14 days. However, qPCR improve the accuracy and sensitivity of detecting Low fungal concentrations 103 conidia g−1 soil in complex environmental matrices like soil. These findings suggest that these primers set have potential for future applications in monitoring specific fungal isolates in the field over period of time. Conclusions The genome sequence of Pl-NCHU-NPUST-175 was decoded and compared with those of closely related isolates and species. Based on a developed comparative genomics approach, we highlight the feasibility of developing specific molecular markers of EPF, and this method could be further applied to commercialize EPF products to address sustainability in the natural environment.

Genomic analyses of Purpureocillium lilacinum reveal unique DNA regions for developing isolate-specific molecular markers

#Purpureocillium

link.springer.com/article/10.1...

Zombie Cicada ♂ #Purpureocillium #Ophiocordycipitaceae #Tanna #冬虫夏草

A man cant even swing through the jungle on vines in peace these days, without having to stop and get out his camera to photograph #Purpureocillium atypicola!

I was hoping to find a magnificent giant banded huntsman host but had to settle for #Purpureocillium on a tiny little spider

The amount of spores #Purpureocillium #anamorphs produce is just insane! This one is on their locally preferred huntsman spider host. Usually found on tree trunks or cliff faces, sometimes on rushes or ferns. The sexual teleomorph state targets spiders in ground burrows like trapdoors.

I have no mouth and I must scream #Purpureocillium #entomopathogenic #zombie #huntsman #spider #fungifriends