Genes for nucleotide synthesis are essential for cell growth, unless nucleosides for salvage are provided. This gave us an idea💡

To identify new players in pyrimidine synthesis, we ran a genome-wide CRISPR-Cas9 screen in media ± uridine, a #pyrimidine #nucleoside.
2/17

#Uridine is the main substrate for #pyrimidine salvage, and uridine supplementation is given to patients with mutations in de novo pyrimidine synthesis #Vistogard #Xuriden 💊

🎯 We screened in +/- uridine and found the three pyrimidine enzymes as hits 1-2-3!

#CAD #DHODH #UMPS

3/9

Global niche partitioning of purine and pyrimidine cross-feeding among ocean microbes Global marine microbial marketplace for DNA building blocks drives biogeochemical cycling and ecological signaling in the ocean.

When thinks start making sense "Global niche partitioning of #purine and #pyrimidine cross-feeding among #ocean #microbes"
doi.org/10.1126/scia...
#cyanobacteria #prochlorococcus #bacteria #marine

Dit is de #Murchison-meteoriet. 1969 ingeslagen in Australië. Bijzonder omdat deze 7 miljard jaren oude ‘steen’ 14000 verschillende organische verbindingen bevat waaronder #Purine und #Pyrimidine, basis bouwstenen van #DNA en RNA. Zijn we dus wel degelijk uit #sterrenstof ontstaan.