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To our knowledge, this is the largest mutational dataset on T7 RNA polymerase to date!

🔗 Access the dataset on the Align Data Portal: hubs.la/Q04802KK0

#OpenScience #SyntheticBiology #ProteinEngineering #BioAI #MachineLearning #AlignData #GROQSEQ #RNApolymerase

REVIEW: Transcription quality control at the promoter-proximal checkpoint
By Daniel Blears and Jesper Svejstrup
➡️ https://genesdev.cshlp.org/content/39/23-24/1399.full

Jesper Svejstrup
Center for Gene Expression (CGEN)

#transcription #promoterproximalpausing #RNApolymerase #elongation #mRNA

Left: Lifespan of females with adult-specific, pan-neuronal induction of dMaf1RNAi(V109142) with 36R driven by Elav-GSTricoire. Right: Model of how Maf1 activity in neurons impacts aging.

#Aging reduces the ability to make new proteins, so can stimulating protein synthesis in old age be beneficial? This study shows that boosting #ProteinSynthesis in #neurons by inhibiting #RNApolymerase repressor Maf1 improves health & survival into old age in flies @plosbiology.org 🧪 plos.io/3IwLiPZ

Left: Lifespan of females with adult-specific, pan-neuronal induction of dMaf1RNAi(V109142) with 36R driven by Elav-GSTricoire. Right: Model of how Maf1 activity in neurons impacts aging.

#Aging reduces the ability to make new proteins, so can stimulating protein synthesis in old age be beneficial? This study shows that boosting #ProteinSynthesis in #neurons by inhibiting #RNApolymerase repressor Maf1 improves health & survival into old age in flies @plosbiology.org 🧪 plos.io/3IwLiPZ

Left: Lifespan of females with adult-specific, pan-neuronal induction of dMaf1RNAi(V109142) with 36R driven by Elav-GSTricoire. Right: Model of how Maf1 activity in neurons impacts aging.

#Aging reduces the ability to make new proteins, so can stimulating protein synthesis in old age be beneficial? This study shows that boosting #ProteinSynthesis in #neurons by inhibiting #RNApolymerase repressor Maf1 improves health & survival into old age in flies @plosbiology.org 🧪 plos.io/3IwLiPZ

The DNA sequence preference of SWR chromatin remodeler at promoters. The SWR complex prefers to remove H2A-H2B dimers (orange) at the side of nucleosomes where there is a poly (dA:dT) tract (AAA…). SWR complex replaces the H2A-H2B dimer with an H2A.Z-H2B dimer (red). The incorporation of H2A.Z-H2B dimer at the side of the nucleosome closest to the start site of transcription causes that side of the nucleosome to be more prone for DNA to unwrap from the histone octamer and for the histone octamer to form a more open conformation. These changes in the nucleosome structure facilitates in transcription initiation.

Gene #promoters are intrinsically hardwired for release of paused #RNApolymerase by H2A.Z; This Primer explores a new @plosbiology.org study showing that poly(dA) tracts at promoters positively stimulate H2A.Z incorporation by the SWR complex in yeast 🧪 Paper: plos.io/4kggWyD Primer: plos.io/4dihoKK

The DNA sequence preference of SWR chromatin remodeler at promoters. The SWR complex prefers to remove H2A-H2B dimers (orange) at the side of nucleosomes where there is a poly (dA:dT) tract (AAA…). SWR complex replaces the H2A-H2B dimer with an H2A.Z-H2B dimer (red). The incorporation of H2A.Z-H2B dimer at the side of the nucleosome closest to the start site of transcription causes that side of the nucleosome to be more prone for DNA to unwrap from the histone octamer and for the histone octamer to form a more open conformation. These changes in the nucleosome structure facilitates in transcription initiation.

Gene #promoters are intrinsically hardwired for release of paused #RNApolymerase by H2A.Z; This Primer explores a new @plosbiology.org study showing that poly(dA) tracts at promoters positively stimulate H2A.Z incorporation by the SWR complex in yeast 🧪 Paper: plos.io/4kggWyD Primer: plos.io/4dihoKK

The DNA sequence preference of SWR chromatin remodeler at promoters. The SWR complex prefers to remove H2A-H2B dimers (orange) at the side of nucleosomes where there is a poly (dA:dT) tract (AAA…). SWR complex replaces the H2A-H2B dimer with an H2A.Z-H2B dimer (red). The incorporation of H2A.Z-H2B dimer at the side of the nucleosome closest to the start site of transcription causes that side of the nucleosome to be more prone for DNA to unwrap from the histone octamer and for the histone octamer to form a more open conformation. These changes in the nucleosome structure facilitates in transcription initiation.

Gene #promoters are intrinsically hardwired for release of paused #RNApolymerase by H2A.Z; This Primer explores a new @plosbiology.org study showing that poly(dA) tracts at promoters positively stimulate H2A.Z incorporation by the SWR complex in yeast 🧪 Paper: plos.io/4kggWyD Primer: plos.io/4dihoKK

CTDP1 and RPB7 stabilize Pol II and permit reinitiation | Nature Communications The mechanisms governing the termination and subsequent reinitiation of RNA polymerase II (Pol II) remain poorly understood. Here we find that depletion of RPB7 leads to the destabilization of Pol II’s largest subunit, RPB1. This destabilization is influenced by the loop regions of RPB7, CDK9, the C-terminal domain (CTD) of RPB1, and its linker region. The stabilization process of RPB1 is regulated by the E3 ubiquitin ligase Cullin 3. Additionally, RPB7 interacts with the phosphatase CTDP1, which is crucial for maintaining RPB1 stability. RPB7 is also vital for the reinitiation of Pol II, engages with RNA processing factors, and is localized to the RNA exit channel of the Pol II complex. The absence of RPB7 compromises RNA processing. We propose that RPB7 recruits CTDP1 to dephosphorylate Pol II, enhancing its stability and facilitating efficient reinitiation, adding an emerging dimension to transcriptional regulation. The mechanisms governing the termination and subsequent reinitiatio

Study reveals RPB7 depletion destabilizes Pol II's RPB1 via loop regions, CDK9, CTD, and linker, regulated by Cullin 3. CTDP1 also plays a role. #RNApolymerase PMID:40038320, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-57513-2 #Medsky #Pharmsky #RNAsky 🧪

Light into the darkness of #photosynthesis: @hauke_hillen, Paula Favoretti Vital do Prado & colleagues @UniHannover have visualized the copying machine of #chloroplasts, the #RNAPolymerase PEP, in high-resolution 3D for the first time. #HorizonEU
https://www.mpinat.mpg.de/4632634/pr_2408

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress

Switching on genes for energy production: Hauke Hillen and Patrick Cramer @CramerLab on their recent work with the Temiakov lab on how #RNApolymerase is regulated in #mitochondria: http://www.mpibpc.mpg.de/15884921/pr_1801 @CellCellPress