Orphan nuclear receptors recruit TRIM28 to promote telomeric H3K9me3 for the ALT pathway - The EMBO Journal Alternative lengthening of telomeres (ALT) is a telomere maintenance mechanism deployed in embryonic stem cells and cancer cells. High levels of the heterochromatin mark H3 lysine 9 trimethylation (H3...

Association of orphan nuclear receptors and heterochromatin are involved in Alternative Lengthening of Telomeres – Liuh-Yow Chen and coworkers identify their link as the transcriptional corepressor TRIM28
Thanks to @reviewcommons.org for another #RefereedPreprint
link.springer.com/article/10.1...

Jennifer A Miles, molecularrichard.bsky.social et al model the Aurora A-Bora-Plk1 ternary complex revealing how Bora bridges both kinases
#RefereedPreprint @reviewcommons.org

link.springer.com/article/10.1...

Molecular basis for the activation of Aurora A and Plk1 kinases during mitotic entry - The EMBO Journal The evolutionarily conserved, intrinsically disordered protein Bora is critical for initiating the activation of mitotic kinases. Once phosphorylated at Ser112 by Cyclin A-Cdk1 kinase, phospho-Bora ac...

AlphaFold modeling & new assays for mitotic kinase activation in E. coli (#MITOKINAC) reveal molecular basis of specific Aurora A & PLK1 activation through Bora during mitotic entry
Lionel Pintard @ccdlab.bsky.social et al
#RefereedPreprint ℅ @reviewcommons.org
link.springer.com/article/10.1...

Molecular requirements for PLK1 activation by T-loop phosphorylation - The EMBO Journal Activation of PLK1, a master mitotic kinase, requires phosphorylation of its activation segment on Thr210, within a basic consensus sequence for Aurora kinases. Aurora B-dependent phosphorylation of T...

Arianna Esposito Verza, @andrea-musacchio.bsky.social et al describe the long-sought-after mechanism of Bora dependency of PLK1 activation by Aurora A kinase during mitotic entry
Another #RefereedPreprint ℅ @reviewcommons.org
link.springer.com/article/10.1...

Ordering of cell cycle events by regulated proteolysis is not just for eukaryotes:
@buzzbaum.bsky.social et al define mechanisms underlying switch-like degradation of archaeal ESCRT-III CvdB in Sulfolobus
Thanks to @reviewcommons.org for another #RefereedPreprint
link.springer.com/article/10.1...

How can gut microbes modulate host lifespan?
@jogender-singh.bsky.social & coworkers show that in C. elegans, lifespan extension arises from microbial perturbation of iron homeostasis and oxidative stress response activation
#RefereedPreprint c/o @reviewcommons.org
www.embopress.org/doi/full/10....

How do cells curb nutrient uptake during quiescence & does it matter?
@teislab.bsky.social & Co. show TXNIP-dependent endocytosis of amino acid transporter LAT1/SLC7A5 is essential to prevent cell death and human disease
#RefereedPreprint c/o @reviewcommons.org
www.embopress.org/doi/full/10....

E. coli bacteriophage N15 suppresses Mre11-Rad50-mediated end degradation of its linear genome via a telomere-resolution-independent function of its telomere resolvase TelN
#RefereedPreprint c/o @reviewcommons.org by @gruberlab.bsky.social
www.embopress.org/doi/full/10....

T-cell smFISH:
@anilevetu.bsky.social, @monikawolkers.bsky.social et al present an optimized workflow for simultaneously quantifying nascent and mature mRNA, and its localization with single-cell resolution in human T-cells
#RefereedPreprint c/o @reviewcommons.org
www.embopress.org/doi/full/10....

Fibril formation of α-synuclein, central to Parkinson’s disease, is catalyzed by ubiquilin-2 droplets and can be suppressed by small-molecule targeting of UBQLN2
#RefereedPreprint c/o @reviewcommons.org by Masaya Imoto, Nobutaka Hattori and coworkers
www.embopress.org/doi/full/10....

#RefereedPreprint c/o @reviewcommons.org by Faccioli, Biasini et al:
Transient exposure of cryptic phosphorylation sites buried in protein cores during folding may represent novel regulatory mechanism linking co-translational modification to protein folding control
www.embopress.org/doi/full/10....

What is the basis & significance of SUMO1 vs SUMO2/3 conjugation preference? Jo Morris et al find that mitotic deacetylation biases SUMO E1 SAE2 towards #SUMO1 conjugation onto NuMA to ensure faithful chromosome segregation
#RefereedPreprint c/o @reviewcommons.org
www.embopress.org/doi/full/10....

Neutralizing antibodies promote filamentous morphology of #influenza A virus by inhibiting cellular entry and transmission of spherical viral particles
 #RefereedPreprint c/o @reviewcommons.org by Petr Chlanda @chlandalab.bsky.social and coworkers
www.embopress.org/doi/full/10....

Early coordination of cell migration and cardiac fate determination during mammalian gastrulation, revealed by light-sheet microscopy tracking of individual mesodermal cells
#RefereedPreprint c/o @reviewcommons.org by @kenzoivanovitch.bsky.social and colleagues
www.embopress.org/doi/full/10....

Another #RefereedPreprint c/o @reviewcommons.org:
@leunglab.bsky.social finds that interferon-induced, PARP14-dependent cytoplasmic ADP-ribose condensates contain autophagy receptor p62, but otherwise depend on the ubiquitin-proteasome system rather than autophagy
www.embopress.org/doi/full/10....

Study by Sarah De Val @saradv.bsky.social @ox.ac.uk and colleagues uncovers key enhancers and regulators of arterial identity! #VascularBiology #GeneRegulation

#RefereedPreprint by @reviewcommons.org
Referee reports and author's response avaliable here >>eeb.embo.org/doi/10.1101/2024.04.30.591717

Using AI and subcellular morphology to predict cell types and molecular traits—no markers needed! Navarro and colleagues explore a new way to understand cell identity and behavior.
#Refereedpreprint at @reviewcommons.org
Reviews and response letter here >>eeb.embo.org/doi/10.1101/2024.08.14.607889

Early Evidence Base | EMBO

Saltel and colleagues @univbordeaux.bsky.social explore how invadosomes recruit ER proteins, driving cell invasion and matrix degradation.

#RefereedPreprint by @reviewcommons.org
Referee comments and author's response posted here >> eeb.embo.org/doi/10.1101/2024.07.02.601728
#OpenScience

See-Yeun Ting 陳詩允 @seeyeunting.bsky.social and colleagues exemplify a critical #evolutionary constraint: while LPS deficiency shields bacteria from phage infection, it heightens vulnerability to contact-dependent antagonism
#RefereedPreprint c/o @reviewcommons.org
www.embopress.org/doi/full/10....

Ancient genetics shape modern human traits. 🦧🧬Check out the latest #Refereedpreprint at @reviewcommons.org from the Long lab ‪on #Neanderthal variants influencing #craniofacialdevelopment
@hannahlong.bsky.social

Referee reports and author's response
>> eeb.embo.org/doi/10.1101/2024.09.24.614243

Drosophila Alms1 proteins are general regulators of #centriolar cartwheel assembly, exhibiting distinct centriolar localization patterns and enabling Plk4-Ana2 interaction
#RefereedPreprint c/o @reviewcommons.org by Véronique Morel, Bénédicte Durand and colleagues
www.embopress.org/doi/full/10....

New #RefereedPreprint c/o @reviewcommons.org:
Daiju Kitagawa, Shoji Hata et al reveal the multimodal, cell cycle-dependent mechanisms coordinating centriole maturation with changes in centriole engagement underlying faithful centrosome duplication
www.embopress.org/doi/full/10....