Less missing values—evaluation of proteomics workflows for the quantification of (small) proteins This study systematically evaluates methods for improving the identification and quantification of small proteins using bottom-up mass spectrometry, thereb

New paper out in microLife: evaluating proteomics workflows to reduce missing values, with a focus on reliable quantification of small proteins. Practical insights for improving coverage and data quality.

academic.oup.com/microlife/ar...

#Proteomics #MassSpectrometry #SmallProteins #Microbiology

🧬 Dive into the small proteome of Methanosarcina mazei (across 5 conditions)! A key resource by @schmitzstreitslab.bsky.social @uni-kiel.de for uncovering #smallproteins functions, motifs and proteoforms in #archaea.

👉 buff.ly/HJOi3E0

Tiny but mighty: Rutgers study shows the 34-aa protein YoaI links phosphate, Mg²⁺, and osmotic stress pathways in E. coli. Small proteins = key cross-talk mediators in bacterial stress signaling. go.njmicrobe.org/1HDIM2 #Microbiology #SmallProteins #StressSignaling #Rutgers

Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates cross-talk between distinct signaling systems Bacterial small proteins (≤ 50 amino acids) are an emerging class of regulators that modulate the activity of signaling networks that enable bacterial adaptation to stress. The Escherichia coli genome...

Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates cross-talk between distinct signaling systems 🦠 from Yadavalli lab. Ribo-seq study of Mg2+ starvation induced 17 small proteins 💫 #RNAsky #microsky #ribosome #smallproteins doi.org/10.1101/2024...

Complementary Ribo-seq approaches map the translatome and provide a small protein census in the foodborne pathogen Campylobacter jejuni - Nature Communications Bacterial genomes still contain many hidden and uncharacterized genes. Using complementary Ribo-seq approaches, Froschauer, Svensson et al. here annotate start and stop codons of Campylobacter jejuni,...

We are happy to share our new paper on the #Campylobacter translatome out now in @naturecomms.bsky.social. Many congrats to @kathrinfroschauer.bsky.social & @campy-bara.bsky.social and all collaboration partners! #SPP2002 #smallproteins @uni-wuerzburg.de

www.nature.com/articles/s41467-025-58329-w

Scientists reveal global catalog of microbial small proteins, unlocking microbiome secrets Researchers developed a global catalog of nearly one billion small open reading frames (smORFs) from microbial genomes, revealing significant protein diversity, particularly in archaea, and providing ...

Scientists reveal global catalog of microbial small proteins, unlocking microbiome secrets 🔬🌍🧬 www.news-medical.net/news/2024090... #Microbiome #Proteomics #smORFs #Genomics #Archaea #Bioinformatics #Microbial #SmallProteins