Preparing for successful protein crystallization experiments
Journal Highlight! 🔊
This article covers strategies for careful sample preparation in crystallization experiments to increase the chance of success.
Find out more: https://ow.ly/ZQzI50Ynt8x
Structural Biology Communications #Crystallization #XRayCrystallography #StructuralBiology
Single-cell structural biology with intracellular electron crystallography
nature.com/articles/s4146…
#StructuralBiology #Crystallography #ElectronDiffraction #3DED #MicroED #XrayCrystallography #CryoEM #FIBSEM #cryoFIB #CLEM #InSitu
The highest resolution DNA-free TaXPD structure to date uniquely resolves loop 510–514 in the long-form, DNA-free TaXPD model #XRayCrystallography #Helicases #Archaea doi.org/10.1107/S205...
#Highlight
Disrupting the SHOC2-RAS Nexus: a new frontier in targeting RAS-Driven cancers doi.org/10.1186/s435...
The highlighted article in @nature.com : doi.org/10.1038/s415...
#Cancer #MedicinalChemistry #XrayCrystallography
Two crystallographic fragment-screening campaigns against SARS-CoV-2 NSP1 identified 21 hits from 192 screened fragments, the highest hit rate reported for Nsp1 to date #XRayCrystallography #SARSCoV2 #CrystallographicFragmentScreening doi.org/10.1107/S205...
We’re heading to #PSDI2025!
Meet the CryoCloud team and don’t miss our talks:
⚡ Sun 9 Nov: Towards Automating Cryo-EM Data Analysis
🚀 Tue 11 Nov: Accelerating Data Analysis with CryoCloud
Drop by our booth or book a chat 👇
connect.cryocloud.io/meetings/cry...
#CryoEM #XrayCrystallography
⏰ Tomorrow!
Join us for the CryoCloud #XRayCrystallography webinar featuring a short introduction to the new pipeline and a panel discussion on today’s challenges and opportunities in high-throughput XRC data processing.
events.teams.microsoft.com/event/6f09a4...
#CryoCloud #StructuralBiology
⏳ 1 Week to Go!
After introducing CryoCloud’s integration of Global Phasing’s Pipedream workflow, we’ll be joined by four experts to discuss the challenges and opportunities in high-throughput #XrayCrystallography.
Register here: events.teams.microsoft.com/event/6f09a4...
#OpenFold3 #BristolMyersSquibb #Takeda #AstexPharma #OpenFoldstructurepredictionplatform #AISBconsortium #AbbVie #JnJ #Apheris #AI #molecularstructurepredictions #GoogleDeepMind #IsomorphicLabs #AlphaFold #biotherapeutics #smallmoleculediscovery #Xraycrystallography #drugdiscovery
zurl.co/jQ7qh
🚀 CryoCloud Platform Release 2.10.1 is here!
This is a big one – CryoCloud now supports X-ray Crystallography alongside #cryoEM 💎
📖 Read the full release notes, including other improvements and bug fixes: connect.cryocloud.io/release-note...
#CryoCloud #XrayCrystallography #StructuralBiology
🚀 New in CryoCloud: Automated X-ray Crystallography Data Processing!
Join our launch webinar on Oct 16 (11 AM ET | 5 PM CET) featuring a CDK2 case study + industry panel on high-throughput XRC.
👉 Register: lnkd.in/ev4e7K_h
#StructuralBiology #XRayCrystallography #XRC #CryoEM
🧬 “Human curiosity… is perhaps the best secret weapon of all.” – Aaron Klug @nobelprize.bsky.social: using #Xraycrystallography & #electronmicroscopy, he revealed DNA, protein & virus structures—showing why #crystallography matters: www.nobelprize.org/prizes/chemi...
Features of sample preparation that should be considered prior to biomolecular crystallization experiments are reviewed, as described in the SAMPREP workshop during the 73rd Annual ACA Meeting #Crystallization #XRayCrystallography #StructuralBiology doi.org/10.1107/S205...
The structure of the C1 domain of the surface-layer protein SlpM from L. brevis provides new insights into the architecture and evolutionary adaptability of SLPs in Lactobacillus species #XRayCrystallography #SurfaceLayerProtein #SlpM doi.org/10.1107/S205...
Why is leadership in structural biology so important? Mark Hargrove shows us how impactful it can be.
www.asbmb.org/asbmb-...
#SciComm #StructuralBiology #ASBMBmemberNews #XRayCrystallography #ProteinStructure
Dorothy Hodgkin, British Biochemist who revolutionized the field of X-ray crystallography! Read: buff.ly/St7N1Ow
Post by Sumbul, Illustration by Miler, Sci-Illustrate Stories. Set in motion by Radhika Patnala.#WomenInScience #WomenInSTEM #NobelPrize #sciart #crystallography #XRayCrystallography
Are X-ray crystallography, cryo-EM, and NMR competitive or complementary?
Each has strengths and limitations—high resolution, flexibility, or dynamic insights. The future of structural biology lies in integration!
What’s your take?
#StructuralBiology #CryoEM #NMR #XrayCrystallography
X-ray Crystallography 🆚 Cryo-EM 🆚 NMR
Are X-ray crystallography, Cryo-EM, and NMR competitive or complementary? 🤔
Think solving a puzzle—do you want sharp atomic details, a near-native image, or a dynamic view?
#StructuralBiology #XrayCrystallography #CryoEM #NMR
#research #XRayCrystallography
CDK2-based CDK7 mimic as a tool for structural analysis: Biochemical validation and crystal structure with SY5609 (Škerlová, Jorda) - Int J Biol Macromol: doi.org/10.1016/j.ij...
@iocbprague.bsky.social #charlesuni #IMTMOlomouc
Hello, I am an Assistant Professor at the Univ of Maryland. We investigate #coronavirus glycoprotein trafficking, using #cryoem #cryoet #xraycrystallography and functional tools. Here is one of our no-longer-recent publications. Great to see a vibrant community!
www.nature.com/articles/s41...
Unlike other serpins, human serpin B9 shows significant structural deviations around helix D, with a larger surface cavity, which could serve as a promising target for small-molecule inhibitors #Serpins #XRayCrystallography #Crystallization t.co/ilB7Aa3Krg
This topical review focuses on 13 X-ray crystal structures and five electron cryo-microscopy structures of glucose-6-phosphate dehydrogenase in the PDB #Glucose6PhosphateDehydrogenase #XRayCrystallography #ElectronCryoMicroscopy doi.org/10.1107/S205...
A method is presented to robustly exclude erroneous reflection measurements arising from beamstop shadows in crystallographic diffraction experiments #XRayCrystallography #AUSPEX #Outliers doi.org/10.1107/S205...
The structure of the Rib domain from Limosilactobacillus reuteri is compared with previously published models and is found to exhibit extraordinary thermal stability #XRayCrystallography #RibDomain #ImmunoglobulinLikeFold t.co/y4cUSd1bHR
A true apo PTPN2 crystal structure with an unbound active site allowed the active site to be observed in a native apo state for the first time and inhibitor complexes were obtained by soaking experiments #PTPN2 #Phosphatases #XRayCrystallography doi.org/10.1107/S205...
Crystallographic evidence is presented for a putative disulfide molecular switch sensitive to the binding of BIA ligands in the opium poppy latex protein PR10-10 #XRayCrystallography #LigandCoupledConformationalChanges #ProteinStructureAndFunction t.co/JacXTgOW9Q
#research #cryoEM #XrayCrystallography
Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function (Řezáčová) - @NAR_Open: doi.org/10.1093/nar/...
@iocbprague.bsky.social
This long Rib domain demonstrates a highly conserved structure and also highlights an evolutionary convergence in structural architecture with other modular domains in cell-adhesion molecules. #BacterialAdhesins #RibDomains #XRayCrystallography t.co/NxObHw6qHN
A structure of carbonic anhydrase II was determined by XFEL serial femtosecond crystallography and is compared with previously reported NMR and synchrotron X-ray and neutron single-crystal structures #CarbonicAnhydraseII #XFEL #XRayCrystallography t.co/7aGMCw9g5j
