SHOT 2.0: CAST-based platform for programmable baculovirus engineering

→ ≥14 kb cargo, dual-loci integration, stable transgene retention (ODVe56 > v-cath)
→ All-in-one Bac-PE5max: 85.6% in HEK293T, 31.3% in iPSCs

📄 pmc.ncbi.nlm.nih.gov/articles/PMC...

#PrimeEditing #Baculovirus #CRISPR

Promega HER2 Kinase Enzyme System - GenomiRX Promega HER2 Kinase Enzyme System

genomirx.com/promega-her2... Promega HER2 Kinase Enzyme System, 1mg baculovirus in Sf9 GST tag @promegacorporation.bsky.social #her2 #kinase #enzyme #baculovirus #sf9 #gst @BPSBioscience @sigmaaldrich.bsky.social @prospec @mybiosource @chemiverseca.bsky.social

New research in #RESInsectMolecBio

Modulation of DNA methyltransferases (#DNMTs) in #Spodoptera frugiperda (Sf9) cells following AcMNPV infection, and its effects on the virus-cell interaction
doi.org/10.1111/imb.70006

#Baculovirus
@sassanasgari.bsky.social @gulianusslab.bsky.social @wiley.com

Smart virus trick enables cleaner vaccine production in insect cells Thanks to a clever modification of the baculovirus, vaccines can now be produced in insect cells more easily and cleaner. Researchers at Wageningen University & Research (WUR) have successfully…

Smart #virus trick enables cleaner #vaccine production in #insect cells ...

| #baculovirus | #VLPs | #bioreactor | #contamination | By @w-u-r.bsky.social‬ via @sciencex.bsky.social‬

Addgene: MoClo Baculo Toolkit Use the MoClo Baculo Toolkit to efficiently generate multigene expression constructs for the baculovirus expression system.

Big thanks to @addgene.bsky.social for making our MoClo Baculo available as a kit! All the plasmids for Golden Gate assembly of baculovirus vectors, now bundled together:
www.addgene.org/kits/davidov...
🧪🧬 #MolecularCloning #baculovirus #ProteinExpression

They published papers on #Baculovirus, a family of insect-infecting viruses with applications in biotechnology, mostly #ProteinExpression. Understanding the viral biology is important, both to understand its interactions with different hosts, and to utilize its feature to develop new technologies.

Check out the magnificent #baculovirus structure! The unique virion architecture and structural atlas of hallmark proteins place baculo-like viruses into a separate new realm. Many insights into baculo biology and evolution. Collab with the group of Fasséli Coulibaly. www.science.org/doi/10.1126/...

Showing a little ‘AMPath-y’ goes a long way!

Show us your #AMPath badge and get ☕️ at booth 1207, set up by incredible women who are my colleagues!

As you warm up, we can chat about #Baculovirus titration, #NucleicAcidPurification, #qPCR, & the great science at Takara Bio USA!

Excited and ready to 'AMP-ress' everyone in beautiful Vancouver this week!

Want to chat about new titration kits for #Baculovirus or maybe about how to automate your #NucleicAcidPurification preps?

I'm your friendly neighborhood Product Manager for these and will be at booth 1207. Come say hi!