Functional sub-states link conformational landscapes and protein evolution The intrinsic conformational flexibility of proteins creates structural heterogeneity, giving rise to conformational ensembles within the energy lands…

🔄 #Enzymes can adopt multiple #conformations.

⚡ Some of these act as functional sub-states.

📚 We summarised recent studies, the methods used to uncover these hidden states, and the challenges ahead in linking them to #proteinevolution.

www.sciencedirect.com/science/arti...

An #OpenSource #automated single-molecule #FRET platform for high-content, #multiwell #plate #screening of #biomolecular #conformations & #dynamics:

Article: doi.org/10.1038/s414...
#autoFRET data analysis: github.com/SchlierfLAB/...
#DIYbio #smFRET #lab #instruments #imaging