SciTech Chronicles. . . . . . . . .May 5th, 2025
bit.ly/stc050525
#neurodegenerative #tau #misfolded #fibrils #nova #TCrB #Red #White #modular #Unitree #"B2 quadruped" #"extreme environment" #"water Cannon" #imaging #D-PSCAN #NTS #VNS #mtDNA #mutant #Disease #heteroplasmy
During RESET, misfolded GPI-APs traffic from the ER to the Golgi. Top left : Time-lapse imaging sequence collected immediately after thapsigargin (TG) treatment of a typical YFP-PrP * NRK cell that was co-expressing ER-marker, Cerulean-calnexin (CER-CNX). Scale bar represents 10 µm. Top right: Plot of the average Pearson’s r values between YFP-PrP * and CER-CNX for 9 individual cells across the 0 and 20 min time points of time-lapses collected after addition of TG. Bottom left: Time-lapse imaging sequence collected immediately after TG-treatment of a typical YFP-PrP * NRK cell that was co-expressing the Golgi marker, CER-GalT. Scale bar represents 10 µm. Bottom right: Plot of the average Pearson’s r values between YFP-PrP * and CER-GalT for 10 individual cells across the 0 and 20 min time points of time-lapses collected after addition of TG.
TMED9 mediates export of misfolded #prion proteins from the ER. @pskcellbio.bsky.social &co show that TMED9 also helps capture #misfolded GPI-anchored proteins that are released by calnexin in the ER and moved into the Golgi for degradation via the RESET pathway @plosbiology.org 🧪 plos.io/44kURu5
During RESET, misfolded GPI-APs traffic from the ER to the Golgi. Top left : Time-lapse imaging sequence collected immediately after thapsigargin (TG) treatment of a typical YFP-PrP * NRK cell that was co-expressing ER-marker, Cerulean-calnexin (CER-CNX). Scale bar represents 10 µm. Top right: Plot of the average Pearson’s r values between YFP-PrP * and CER-CNX for 9 individual cells across the 0 and 20 min time points of time-lapses collected after addition of TG. Bottom left: Time-lapse imaging sequence collected immediately after TG-treatment of a typical YFP-PrP * NRK cell that was co-expressing the Golgi marker, CER-GalT. Scale bar represents 10 µm. Bottom right: Plot of the average Pearson’s r values between YFP-PrP * and CER-GalT for 10 individual cells across the 0 and 20 min time points of time-lapses collected after addition of TG.
TMED9 mediates export of misfolded #prion proteins from the ER. @pskcellbio.bsky.social &co show that TMED9 also helps capture #misfolded GPI-anchored proteins that are released by calnexin in the ER and moved into the Golgi for degradation via the RESET pathway @plosbiology.org 🧪 plos.io/44kURu5
During RESET, misfolded GPI-APs traffic from the ER to the Golgi. Top left : Time-lapse imaging sequence collected immediately after thapsigargin (TG) treatment of a typical YFP-PrP * NRK cell that was co-expressing ER-marker, Cerulean-calnexin (CER-CNX). Scale bar represents 10 µm. Top right: Plot of the average Pearson’s r values between YFP-PrP * and CER-CNX for 9 individual cells across the 0 and 20 min time points of time-lapses collected after addition of TG. Bottom left: Time-lapse imaging sequence collected immediately after TG-treatment of a typical YFP-PrP * NRK cell that was co-expressing the Golgi marker, CER-GalT. Scale bar represents 10 µm. Bottom right: Plot of the average Pearson’s r values between YFP-PrP * and CER-GalT for 10 individual cells across the 0 and 20 min time points of time-lapses collected after addition of TG.
TMED9 mediates export of misfolded #prion proteins from the ER. @pskcellbio.bsky.social &co show that TMED9 also helps capture #misfolded GPI-anchored proteins that are released by calnexin in the ER and moved into the Golgi for degradation via the RESET pathway @plosbiology.org 🧪 plos.io/44kURu5
