Novel Nitric Oxide Dismutase Drives Effective NO Degradation in Oxygenic Denitrification Nitrogenous wastewater is a major environmental problem. Denitrification refers to sequential reduction of nitrate (NO3-) and nitrite (NO2-) to nitric oxide (NO

Novel #Nitric Oxide Dismutase Drives Effective NO Degradation in Oxygenic #Denitrification

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Spatio-temporal distribution and production-consumption dynamics of #nitric oxide in coastal waters off Qingdao, China www.sciencedirect.com/science/arti...

Isotopic Fractionation and Kinetic Isotope Effects of a Purified Bacterial Nitric Oxide Reductase (NOR) Nitrous oxide (N2O) is a serious concern due to its role in global warming and ozone destruction. Agricultural practices account for ∼80% of all anthropogenic N2O produced in the US, due in large part to the stimulation of microbial denitrification. Stable isotopes are uniquely suited to examine both microbial N2O sources and the mechanism of N2O biosynthesis through the use of Site Preference (δ15NSP; the difference in δ15N between the central and outer N atoms in N2O) and kinetic isotope effects (KIEs), respectively. Using trace gas isotope ratio mass spectrometry (TG-IRMS), we determined the δ15N, δ15Nα, δ15Nβ, and δ18O of N2O produced by a purified cytochrome c nitric oxide reductase (cNOR) from Paracoccus denitrificans. We also calculated δ15NSP, the KIEs, and associated isotopic enrichment factors (ε) for Nbulk, Nα, and Nβ. A normal isotope effect was observed for bulk 15N, with a KIE value of 1.0086 ± 0.0009 (ε = −8.6 ± 0.9‰). The isotope effects for both 15Nα and 15Nβ were also normal, with position-specific KIEs of 1.0072 ± 0.0010 (ε = −7.2 ± 1.0‰) and 1.0100 ± 0.0010 (ε = −9.9 ± 1.0‰), respectively, and δ15NSP values ranged from 0.5 to 8.7‰ with no significant trend as the reaction proceeded. Values of δ18O increased with N2O production (slope of δ18O against [−f ln f/(1 – f)] = −19.9 ± 1.9‰). We present implications for the mechanism of N2O production from cNOR based on our data.

Isotopic Fractionation and Kinetic Isotope Effects of a Purified Bacterial #Nitric Oxide Reductase (NOR)
pubs.acs.org/doi/10.1021/...

Production of NO and N2O during shortcut nitrification: characteristics and mechanisms Nitric oxide (NO) and nitrous oxide (N2O) are significant greenhouse gases, yet their production mechanisms remain inadequately understood. To elucidate the production mechanisms of NO and N2O duri...

Production of NO and N2O during shortcut #nitrification: characteristics and mechanisms #nitric #nitrous
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Transforming competitors into cooperators: Nitrospira provide nitric oxide for Candidatus Brocadia to achieve synergistic nitrogen removal Nitrospira was frequently accumulated in the mainstream partial nitrification/anammox (PN/A) systems and competed with anaerobic ammonium oxidation ba…

Transforming competitors into cooperators: #Nitrospira provide #nitric oxide for Candidatus #Brocadia to achieve synergistic #nitrogen removal www.sciencedirect.com/science/arti...

#Nitric oxide-forming #nitrite reductases in the anaerobic ammonium oxidizer Kuenenia stuttgartiensis #anammox
NirS isn't so bad after all
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The Flavohemoglobin Hmp and Nitric Oxide Reductase Restrict Initial nir Expression in the Bet‐Hedging Denitrifier Paracoccus denitrificans by Curtailing Hypoxic NO Signalling This study explores the transition from aerobic respiration to denitrification in Paracoccus denitrificans and the role of NO and its enzymatic sinks in the induction of NirS and bet-hedging. We show....

The Flavohemoglobin Hmp and #Nitric Oxide Reductase Restrict Initial nir Expression in the Bet-Hedging Denitrifier #Paracoccus denitrificans by Curtailing Hypoxic NO Signalling #denitrification enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...

Cold seeps are potential hotspots of deep-sea #nitrogen loss driven by microorganisms across 21 phyla #nitric oxide dismutase #hydrazine synthase #anammox #methylomirabilis #nitrous nosZ diversity
www.nature.com/articles/s41...

Impact of salinity gradients on nitric oxide emissions and functional microbes in estuarine wetland sediments Estuarine wetland sediments are hotspots for nitrogen cycling and critical sources of atmospheric nitric oxide (NO). Yet studies on the impact of sedi…

Impact of salinity gradients on #nitric oxide emissions and functional microbes in estuarine wetland #sediments
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The Ocean Serves as a Net Source of Atmospheric #Nitric Oxide but a Net Sink of #Nitrogen Dioxide pubs.acs.org/doi/10.1021/...

Active and abundant alphaproteobacterial #nitric oxide transforming enzymes in a marine oxygen deficient zone.
NO dismutase
Rhodosprilla
Planctomycetes

www.biorxiv.org/content/10.1...