Ground breaking new article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#PlantandEnvironmentalScience
#CRISPR–Cas
#Nuclease
#TransgenicLine
#GuideRNA
#gRNA
#Agrobacterium
Fig.1c
Ground breaking new article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#PlantandEnvironmentalScience
#CRISPR–Cas
#Nuclease
#TransgenicLine
#GuideRNA
#gRNA
#Agrobacterium
Fig.1a
Ground breaking new article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#PlantandEnvironmentalScience
#CRISPR–Cas
#Nuclease
#TransgenicLine
#GuideRNA
#gRNA
Caption to fig.1a
Phylogenetic analysis of Artemis, SNM1A and SNM1B across eukaryotes. Top left: A Bayesian tree was constructed by MrBayes based on the MBL and β-CASP domains of Artemis, SNM1A and SNM1B. Numbers indicate the posterior probability. Top right: a multiple sequence alignment of Artemis proteins, alongside mouse SNM1A and SNM1B. Conserved residues were highlighted by red background. Black stars indicated the conserved residues composing motif I-IV and motif A-C in Artemis. Bottom: Intron phase composition of Artemis from invertebrate and vertebrate representatives was shown. On the left, colored rectangles in light orange, light blue and light yellow represent three distinct intron phases, respectively. On the right, colored rectangles in orange, light green and gray correspond to the MBL, β-CASP and C-terminus domains of Artemis protein, respectively.
Evolutionary analysis of the #nuclease Artemis suggests an important role in the domestication of RAG-like #transposons, such as that which gave rise to V(D)J recombination. Conserved residues crucial for nuclease activity are also identified @plosbiology.org 🧪 plos.io/3RuyNpl
Phylogenetic analysis of Artemis, SNM1A and SNM1B across eukaryotes. Top left: A Bayesian tree was constructed by MrBayes based on the MBL and β-CASP domains of Artemis, SNM1A and SNM1B. Numbers indicate the posterior probability. Top right: a multiple sequence alignment of Artemis proteins, alongside mouse SNM1A and SNM1B. Conserved residues were highlighted by red background. Black stars indicated the conserved residues composing motif I-IV and motif A-C in Artemis. Bottom: Intron phase composition of Artemis from invertebrate and vertebrate representatives was shown. On the left, colored rectangles in light orange, light blue and light yellow represent three distinct intron phases, respectively. On the right, colored rectangles in orange, light green and gray correspond to the MBL, β-CASP and C-terminus domains of Artemis protein, respectively.
Evolutionary analysis of the #nuclease Artemis suggests an important role in the domestication of RAG-like #transposons, such as that which gave rise to V(D)J recombination. Conserved residues crucial for nuclease activity are also identified @plosbiology.org 🧪 plos.io/3RuyNpl
Phylogenetic analysis of Artemis, SNM1A and SNM1B across eukaryotes. Top left: A Bayesian tree was constructed by MrBayes based on the MBL and β-CASP domains of Artemis, SNM1A and SNM1B. Numbers indicate the posterior probability. Top right: a multiple sequence alignment of Artemis proteins, alongside mouse SNM1A and SNM1B. Conserved residues were highlighted by red background. Black stars indicated the conserved residues composing motif I-IV and motif A-C in Artemis. Bottom: Intron phase composition of Artemis from invertebrate and vertebrate representatives was shown. On the left, colored rectangles in light orange, light blue and light yellow represent three distinct intron phases, respectively. On the right, colored rectangles in orange, light green and gray correspond to the MBL, β-CASP and C-terminus domains of Artemis protein, respectively.
Evolutionary analysis of the #nuclease Artemis suggests an important role in the domestication of RAG-like #transposons, such as that which gave rise to V(D)J recombination. Conserved residues crucial for nuclease activity are also identified @plosbiology.org 🧪 plos.io/3RuyNpl
