#onlywithHCR: LaBelle et al. reveal that cell–cell collisions are drivers of the dispersal of endodermal cells in zebrafish embryos. With #HCRtechnology, they observed epha2a expression, finding that endodermal cells undergo RhoA-dependent, active repulsion-type CIL.
Read more: tinyurl.com/mrx934sc
#onlywithHCR: Diaz Soria et al. used scRNA-seq with #HCRtechnology to build a cellular atlas of the mother sporocyst, spatially validating 6 major cell types and revealing key regulators of early parasite growth and snail-host interaction.
Read the full study @nature.com: tinyurl.com/bp7dbsjx
#onlywithHCR: Goldman et al. created the Aedes aegypti Mosquito Cell Atlas, revealing novel cell types, the organization of sensory neuron chemoreceptors, and sexually dimorphic gene expression in the antenna and brain.
🦟 Read more: tinyurl.com/2rtk4bz2
#onlywithHCR: Gillis et al. used #HCRtechnology to visualize gene expression during gill slit formation in skate embryos, revealing a precise sequence of epithelial interactions between the endoderm and ectoderm.
🔬 Read more: tinyurl.com/3mvj2wu2
#onlywithHCR: Rajan et al. illuminated how multicellular dynamics shape olfactory neurogenesis in zebrafish. These findings show how coordinated signaling supports ongoing neuron production in a complex organ system.
Read more: tinyurl.com/3tpmf4v3
#onlywithHCR: Romero et al. explored how pathogenic TP53 variants contribute to congenital anomalies of the kidney and urinary tract and genital defects. Using Xenopus embryos, they observed that the expression of mutant TP53 disrupted kidney morphogenesis.
Read more here: tinyurl.com/mr3yfwzy
#onlywithHCR: Bhandiwad et al. utilized #HCRtechnology to map the neural activity underlying tonic immobility. They found that this outlines a complete sensorimotor circuit, revealing how the brainstem circuit suppresses movement when facing threats.
Read more: tinyurl.com/4dcxhs38
#onlywithHCR: Monroy et al. investigated the timing and localization of key transcription factors during neural crest (NC) development in chicken, quail, and peafowl. They found that quail NC cells initiate expression of SNAI2, SOX9, and SOX10 at earlier stages.
Read more: tinyurl.com/yt4fycah
#onlywithHCR: Sun et al. uncovered a crucial role for Zic5 in Xenopus eye development. Using #HCRtechnology, they showed Zic5 expression in the RPE and NR at the optic vesicle stage, where it helps tune Hedgehog signaling to ensure proper eye development.
Read more: tinyurl.com/mrxru323
#onlywithHCR: Busby et al. utilized #HCRtechnology to track gene expression during chicken somitogenesis. They revealed a stalled cell state linked to Hox genes, indicating that developmental timing is set intrinsically but can also be influenced by extrinsic cues.
Read more: tinyurl.com/pxs5jrcu
#onlywithHCR: Janesick et al. created an RNA-seq atlas of the chicken cochlea, revealing regenerative insights. Using #HCRtechnology to validate gene expression, they found tonotopic gradients along the cochlea, setting up the stage for comparative studies.
Read more: tinyurl.com/2t7zhfwn
#onlywithHCR: With #HCRtechnology, Witch et al. revealed that salamander brain comparisons point to the DVR and neocortex evolving through convergence, while the mammalian olfactory-entorhinal-hippocampal circuit may trace back to ancestral origins.
Read more: tinyurl.com/4bwm599u
#onlywithHCR: Treen et al. pinpointed two Prdm1 homologs as the earliest FGF-responsive genes. By combining #HCRtechnology with genetic and experimental manipulations, they found a sharp developmental transition between the 8- and 16-cell stages.
Read more: genesdev.cshlp.org/content/37/3...
#onlywithHCR: Kim et al. dissected how human pyramidal neurons connect to inhibitory interneurons in a cell type-specific manner. Using #HCRtechnology to confirm cell types and gene expression, they discovered conserved synaptic motifs in human brain circuits.
Read more: tinyurl.com/4nrsm275
#onlywithHCR: Lin et al. mapped ~500K nuclei across 19 CNS regions in marmoset to chart glial diversity.
With #HCRtechnology, they visualized key gene expression patterns in OLIG2+ cells, revealing transcriptional shifts in white matter glia within a 3D context.
Read more: tinyurl.com/bdfadjnr
#onlywithHCR: Colquitt et al. mapped how the loss of auditory feedback destabilizes learned motor behaviors. Using #HCRimaging, they were able to observe the widespread gene expression changes across the birdsong sensorimotor circuit caused by deafening.
🔬Read the full story: tinyurl.com/3fb2hpps
#onlywithHCR: Task et al. mapped the expression of all major chemosensory co-receptors in Drosophila melanogaster. This revealed that Ir25a is co-expressed with Orco, suggesting a more integrated receptor network.
Explore the full story here: elifesciences.org/articles/725...
#onlywithHCR: In a remarkable study, O’Gorman et al. explored how traits evolve in tandem in Astyanax mexicanus. The team mapped oca2 gene expression in hybrid fish—uncovering a shared molecular signature in pigmentation and sleep behavior.
Dive into the full story here: tinyurl.com/4uya4xzz
#onlywithHCR: In a cross-species study, Guillotin et al. unveiled how domesticated grasses evolved distinct traits through fine-scale changes in root cell types, revealing orthologous gene markers and highlighting which cell types evolved rapidly.
Read the full story here: tinyurl.com/peny8r64
#onlywithHCR: Morales et al. explore the hidden world of bacterial–fungal interactions (BFI). Using #HCRimaging, the team overcame key barriers in detecting endohyphal bacteria and visualized bacterial presence within fungal structures.
Explore the full findings here: tinyurl.com/ya5vpy66
#onlywithHCR: Schember et al. compared the regulatory landscapes of the single-minded (sim) gene in two divergent insects. Using #HCRimaging, their findings highlight the evolutionary rewiring of the gene regulatory network.
Read the full study here: journals.plos.org/plosgenetics...
#onlywithHCR: In a fascinating study, Lopez and Alward used #HCRimaging to visualize cyp19a1 (brain-specific aromatase) mRNA expression in A. burtoni, providing new insight into how androgen signaling may influence social behavior.
Read the full study here: pmc.ncbi.nlm.nih.gov/articles/PMC...
#onlywithHCR: Gray and Lovely leveraged #HCRimaging to fluorescently visualize rar and rxr mRNA in transgenic zebrafish embryos, revealing overlapping expression of multiple rar/rxr genes between 12–32 hours post-fertilization.
Read the full study here: tinyurl.com/5crp99fs
#onlywithHCR: Moriano-Gutierrez et al. uncovered how the bacterial small RNA SsrA enables Vibrio fischeri to maintain a stable symbiosis with its squid host, Euprymna scolopes. By using HCR™ imaging, they visualized how SsrA is packaged into outer membrane vesicles.
Read more: tinyurl.com/4fyuyjrp
#onlywithHCR: Najle et al. reveal that fundamental neuronal and cell type programs arose prior to the evolution of true neurons. Leveraging HCR™ imaging, they mapped conserved cell type expression programs.
Explore the full study here: tinyurl.com/2hx9zrd5
#onlywithHCR: Rees et al. have mapped how gill slits form in the skate through a stereotypical sequence of epithelial interactions. Applying HCR™ technology, the authors found that epithelial remodeling and gill slit perforation fail without Fgf signaling.
Read the study here: tinyurl.com/b4ceh3a6
#onlywithHCR: Mapping Epithelial Colonization by Fusobacterium nucleatum in Complex Lung Samples
In a compelling new manuscript, Moore et al. uncover surprising insights into Mycobacterium tuberculosis (Mtb) colonization in the human lung. 🫁
Explore the full study here: shorturl.at/K7gVU
#onlywithHCR: A study by Guillotin et al. reveals how different grass species evolved distinct traits, driven by specialized cell types. #HCRimaging was used to visualize gene expression patterns, uncovering how cell types evolved through gene module recruitment.
Learn more: tinyurl.com/peny8r64
#onlywithHCR: @degrainger.bsky.social, @oliverastone.bsky.social, and their team reveal that lymphatic endothelial cells arise from specialized progenitors. #HCRimaging helped them to visualize endothelial progenitors in whole-mount mouse embryos.
Learn more: www.nature.com/articles/s44...
