Greetings from the Freiburg moss group.
#Physcomitrella #Sphagnum #Takakia

Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella - Plant Cell Reports Key message The evolutionary conserved TOR kinase positively controls growth and development of the moss Physcomitrella, development and function of its chloroplasts, its protein synthesis, and cell-c...

Please check out:
Functional characterisation of Target of Rapamycin (TOR) signalling in #Physcomitrella
We found a remarkable evolutionary conservation of this central #signalling hub, but also specific differences, esp. in RAPTOR.
link.springer.com/article/10.1...

It's officially out 🥳🥳🥳 doi.org/10.1016/j.cu... #MorphoGraphX #MorphoDynamX #Physcomitrella #Phyllotaxis @roederlab.bsky.social @richardsmithlab.bsky.social

tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella - Plant Methods CRISPR/Cas9-based genome editing in the model bryophyte Physcomitrium patens (commonly known as Physcomitrella) is widely used for gene knockout via small insertions or deletions (indels). In this stu...

Great method development!
tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the #moss #Physcomitrella
link.springer.com/article/10.1...

Very interesting preprint!
Evolution of moss leaf-like organs through variations in deeply conserved developmental principles
#auxin #Physcomitrella
www.biorxiv.org/content/10.6...

Interesting preprint:
Effects of microgravity on the three-dimensional morphology of rhizoids in Physcomitrium patens
#Physcomitrella
www.biorxiv.org/content/10.6...

Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella - Plant Cell Reports Key message The evolutionary conserved TOR kinase positively controls growth and development of the moss Physcomitrella, development and function of its chloroplasts, its protein synthesis, and cell-c...

Happy to share our latest publication:
Functional characterisation of Target of Rapamycin (TOR) signalling in #Physcomitrella
We close the knowledge gap about the evolutionary conserved #TOR kinase in non-vascular plants, such as mosses.
link.springer.com/article/10.1...

Secretion-based production of prolyl-hydroxylated human type III collagen in scalable Physcomitrella photobioreactors - Plant Cell Reports Key message Scalable moss bioreactors enable the production of high-quality recombinant prolyl-hydroxylated human collagen without heterologous P4H expression, offering a sustainable and vegan alterna...

Happy to share our latest publication:
Secretion-based production of prolyl-hydroxylated human type III collagen in scalable #Physcomitrella photobioreactors
We present a #sustainable and #vegan alternative to conventional collagens for use in #cosmetics.
link.springer.com/article/10.1...

Secretion-based production of prolyl-hydroxylated human type III collagen in scalable Physcomitrella photobioreactors Collagens are structural proteins of the extracellular matrix essential for skin elasticity and integrity. They are widely used in dietary supplements and cosmetics. Conventional collagens of animal o...

Please check out our improved preprint
Secretion-based production of prolyl-hydroxylated human type III collagen in scalable Physcomitrella photobioreactors
This another good example for how #Physcomitrella can produce difficult-to-express proteins.
#vegan #collagen.
www.biorxiv.org/content/10.1...

Functional Characterization of Target of Rapamycin (TOR) Signalling in Physcomitrella Target of rapamycin (TOR) is a conserved protein kinase and an important signalling hub in eukaryotes. The moss Physcomitrella (Physcomitrium patens) is a model organism for plant physiology, developm...

Check out our improved preprint:
Functional Characterization of Target of Rapamycin (#TOR) Signalling in #Physcomitrella
with more results, e.g. a phylogenetic analysis of #RAPTOR genes.
Happy to see that this preprint already received 2 citations.
www.biorxiv.org/content/10.1...

Physcomitrium patens: an emerging model for autophagy study - Protoplasma Physcomitrium patens (formerly Physcomitrella patens), a model moss species, has emerged as an invaluable system for studying autophagy in plants. This review highlights the unique advantages of P. pa...

Interesting review:
Physcomitrium patens: an emerging model for autophagy study
#physcomitrella #moss
link.springer.com/article/10.1...

Frontiers | CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes Although plants share core cell division mechanisms with other eukaryotes, their unique features, such as acentrosomal spindle formation and cytokinesis via ...

Happy to share our latest publication:
CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes
#moss #Physcomitrella #celldivision
www.frontiersin.org/journals/pla...

Production of prolyl-hydroxylated human type III collagen in Physcomitrella photobioreactors Collagens are important structural proteins of the extracellular matrix that are essential for skin elasticity and integrity and are widely used in dietary supplements and cosmetics. Conventional coll...

Please check out our latest preprint:
Production of prolyl-hydroxylated human type III collagen in Physcomitrella photobioreactors
This #vegan #collagen is another good example how #Physcomitrella can produce difficult-to-express proteins to meet consumer needs.
www.biorxiv.org/content/10.1...

Extreme environmental tolerance and space survivability of the moss, Physcomitrium patens Biological sciences; Plant biochemistry; Plant Biology; Plant development; Plant physiology; Plants; Space sciences

I really miss working with #moss. So many open questions in #Physcomitrella, yet the model keeps falling behind others plant and animal ones.
When funding narrows to a few trendy topics, research diversity collapses progress slows down. www.cell.com/iscience/ful...

A #Physcomitrella photobioreactor
#mossmonday #BlueSkyMossFotos

Functional Characterization of Target of Rapamycin (TOR) Signalling in Physcomitrella Target of rapamycin (TOR) is a conserved protein kinase and an important signalling hub in eukaryotes. The moss Physcomitrella (Physcomitrium patens) is a model organism for plant physiology, developm...

Happy to share our latest preprint:
Functional Characterization of Target of Rapamycin (TOR) Signalling in Physcomitrella
@cibss.bsky.social #Physcomitrella #moss
Please check it out.
www.biorxiv.org/content/10.1...

CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes Although plants share core cell division mechanisms with other eukaryotes, their unique features, such as acentrosomal spindle formation and cytokinesis via the phragmoplast, suggest the existence of ...

Please check out our improved #preprint:

CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes

Thank you, Elena Kozgunova for taking the lead.

#Physcomitrella #celldivision

www.biorxiv.org/content/10.1...

Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors - Plant Cell Reports Key message First production of virus-like particles as a vaccine candidate in a non-vascular plant. Abstract Virus-like particles (VLPs) are self-assembling nanoparticles composed of viral structural...

Happy to share our latest publication:
Production of human papillomavirus type 16 virus-like particles in #Physcomitrella #photobioreactors
It's the first report on the production of #VLPs as #vaccine candidates in a non-vascular plant.
link.springer.com/article/10.1...

Submitted and out as preprint: A Plausible Model of Phyllotaxis Patterning from a Single Apical Cell. 🪴🌱 Including 3D cell analysis & modeling #MorphoGraphX #MorphoDynamX #Physcomitrella #Phyllotaxis @roederlab.bsky.social @richardsmithlab.bsky.social dx.doi.org/10.2139/ssrn...

A robustly rooted tree of eukaryotes reveals their excavate ancestry - Nature The root of the eukaryote Tree of Life is estimated from a new, larger dataset of mitochondrial proteins including all known eukaryotic supergroups, showing it lies between two multi-supergroup assemb...

Interesting read, not only because #moss #Physcomitrella is used as the signature plant:
A robustly rooted tree of eukaryotes reveals their excavate ancestry
www.nature.com/articles/s41...

CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes Although plants share core cell division mechanisms with other eukaryotes, their unique features – such as acentrosomal spindle formation and cytokinesis via the phragmoplast – suggest the existence o...

Happy to share our latest preprint @biorxivpreprint.bsky.social:
CRISPR/Cas9 targeted genetic screening in #Physcomitrella identifies novel cell division genes.
@biologyunifreiburg.bsky.social @cibss.bsky.social
www.biorxiv.org/content/10.1...

Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors Human papillomavirus (HPV) infections are the main causal agent of cervical and other anogenital cancers. Modern HPV vaccines are based on virus-like particles (VLPs), which are self-assembling nanopa...

Our updated preprint now on bioRxiv:
Production of human papillomavirus type 16 virus-like particles in #Physcomitrella photobioreactors
www.biorxiv.org/content/10.1...

Check out our latest research on Physcomitrella FtsZ proteins! #FtsZ #Physcomitrella @chrisvanderdoes.bsky.social @reskilab.bsky.social

Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins - Scientific Reports Scientific Reports - Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins

Happy to share our latest publication:
Differential GTP-dependent in-vitro polymerization of recombinant #Physcomitrella FtsZ proteins
#livMatS @cibss.bsky.social @biologyunifreiburg.bsky.social
www.nature.com/articles/s41...

Physcomitrium LATERAL SUPPRESSOR genes promote formative cell divisions to produce germ cell lineages in both male and female gametangia The evolution of green plants from aquatic to terrestrial environments is thought to have been facilitated by the acquisition of gametangia, specialized multicellular organs housing gametes. Antheri...

Great new paper in @newphyt.bsky.social:
Physcomitrium LATERAL SUPPRESSOR genes promote formative cell divisions to produce germ cell lineages in both male and female gametangia
#Physcomitrella #evodevo
nph.onlinelibrary.wiley.com/doi/10.1111/...

Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins Bacterial cell division and plant chloroplast division require self-assembling Filamentous temperature-sensitive Z (FtsZ) proteins. FtsZ proteins are GTPases sharing structural and biochemical similar...

Happy New Year!
Our preprint
Differential GTP-dependent in-vitro polymerization of recombinant #Physcomitrella FtsZ proteins
www.biorxiv.org/content/10.1...
has been accepted for publication.
Stay tuned.

Recombinant production of spider silk protein in Physcomitrella photobioreactors Spider dragline silk stands out as a remarkable biomaterial, representing one of nature's toughest fibres. Its strength rivals that of many synthetic fibres used commercially, rendering it applicable ...

Happy to present our latest #bioRxiv #preprint:
Recombinant production of spider silk protein in #Physcomitrella photobioreactors.
#biotech #smartmaterials #spidersilk
www.biorxiv.org/content/10.1...

Our press release #UniFreiburg for transcriptional gene silencing by #microRNA in #Physcomitrella from 2010:
Micro RNAs make Genes shut up
pr.uni-freiburg.de/pm/2010/pm.2...
Our publication in Cell:
Transcriptional Control of Gene Expression by MicroRNAs
sciencedirect.com/science/arti...

A snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

New preprint, now with full text:
A snapshot of the #Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins
#ndegron #proteases #moss
www.biorxiv.org/content/10.1...

Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases Hydroxylation of prolines to 4-trans-hydroxyproline (Hyp) is mediated by prolyl 4-hydroxylases (P4Hs). In plants, Hyps occur in Hydroxyproline-rich gl…

Delighted to present our latest publication:
Differential prolyl hydroxylation by six #Physcomitrella prolyl-4 hydroxylases
#glycoengineering #biopharmaceuticals #ispmf
www.sciencedirect.com/science/arti...