Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection Does HIV-1 package host proteins that promote infection? Using in virion RNA interactome capture, Garcia-Moreno et al. mapped human proteins bound to the viral genome inside virions. They identified o...

We used our new ivRIC method to map host proteins inside HIV-1 particles, finding 100+ proteins that the virus hijacks to boost infection.

This project has a long history, spanning from pre-COVID to now! Driven by Manuel Garcia-Moreno and led by @alf-castello.bsky.social

tinyurl.com/54vb842w

RNA binding as an emerging regulatory dimension in the type I IFN response Iselin et al. explore how the type I IFN response regulates RNA-binding activity using RNA-interactome capture and extend the RIC protocol to assess the role of phosphorylation in this process. Focusi...

Proud of Louisa Iselin for this work. Not only a covid baby but had both of her supervisors move lab (@alf-castello.bsky.social and me). The works is a meticulous analysis of the interferon pathway and specifially how RNA binding of proteins are modulated by PTMs.

www.cell.com/cell-reports...

She also discovered a phosphosite in MATR3 that regulates its affinity for RNA after interferon stimulation 🤯

we also report an approach (phospho-RIC) to map phosphosites in RNA-binding proteins and define their influence RNA-binding 🏆

RNA binding as an emerging regulatory dimension in the type I IFN response Iselin et al. explore how the type I IFN response regulates RNA-binding activity using RNA-interactome capture and extend the RIC protocol to assess the role of phosphorylation in this process. Focusi...

Proud of Louisa Iselin. Not the easiest PhD, covid, two supervisors (shabazlab.bsky.social) moving labs, but she pushed through and delivered a really solid piece of work on how interferon reshapes RNA-protein interactions post-translationally www.cell.com/cell-reports...

6/ This study is a collaborative effort with Arvind H. Patel & @alf-castello.bsky.social - @cvrinfo.bsky.social; Rafael Sanjuán - @i2sysbio.es & Miguel Chillón - @uab.cat

👇 Check the original article published in @natcomms.nature.com www.nature.com/articles/s41...

Delighted to collaborate with @melisupf.bsky.social on this exciting study. Juana Diez and her team reveal that coronaviruses reprogram the cellular tRNA pool to optimise viral gene expression 🤯🍾🥂🥳🥳

Identifiquen una diana terapèutica que permetria frenar la propagació dels coronavirus Es tracta d'un enzim que s'activa en les cèl·lules humanes com a resposta a l'estrès degut a la infecció i provoca canvis en la maquinària cel·lular que afavoreixen la producció i la propagació dels c...

1/ 🎯 A therapeutic target that would curb the spread of #coronaviruses has been identified

It is an enzyme that is activated in human cells in response to stress due to infection and causes changes in the cellular machinery that favour the production and spread of coronaviruses
tuit.cat/5y9Rr

Thrilled to contribute to this awesome work led by Ben Davis and @shabazlab.bsky.social on in situ mapping of the in cellulo cysteinome, which opens lots of exciting applications www.nature.com/articles/s41...

Thrilled to contribute to @samjwilsonphd.bsky.social’s new Science paper showing that avian flu polymerase, via its PB1 subunit, stays active at fever temperatures, unlike human flu polymerase. A key factor in their different pathology. 🤯🍾🥂🎉

Thrilled to announce the successful thesis viva defense of Zaydah Roland de Laurent 🦟 on her work defining the RNA interactome of mosquitoes. So proud for her successful defence! Well deserved! #MosquitoResearch #RNA #Doctorate.

We’ll soon be advertising a postdoc position (3 years) to study how mRNA-specific translational regulation shapes the innate immune system, in collaboration with @alf-castello.bsky.social. The formal ad will be out soon but get in touch if you’d like an informal chat! #RNASky

Are you thinking on an exciting PHD project at the interface of translation control and innate immunity? @rnanomad.bsky.social and I have a project that might interest you!

Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells Author summary In host cells, RNA-binding proteins play essential roles in the life cycle of many viruses by influencing how viral RNA is processed, translated, replicated and assembled into virions. ...

Our new PLOS Pathogens paper with ‪@brennanlab.bsky.social‬ is out! We charted the first protein–RNA interaction map for virus-infected tick cells, revealing 283 RNA-binding proteins reshaped by Uukuniemi virus. Some directly control viral particle output. Full story: doi.org/10.1371/jour...

Post 5/5 🧵
IMPACT: This transforms tick-borne disease research:
🎯 New drug targets
🎯 Understanding vector competence
🎯 Virus-tick evolution
Testing if mechanisms work across other tick viruses next.

👨‍🔬 collaborative project with @alf-castello.bsky.social and @alainkohlvirology.bsky.social

🚀 New from Cell: SPIDR is a game-changer—map dozens to hundreds of RNA-binding proteins simultaneously with single-nucleotide precision in one CLIP experiment. An exciting advance for RNA–protein interactomics! 🧬🔗https://doi.org/10.1016/j.cell.2025.06.042

Integrating Alternative Fragmentation Techniques into Standard LC-MS Workflows Using a Single Deep Learning Model Enhances Proteome Coverage We built and characterised a mass spectrometer capable of performing CID (both beam type and resonant type), UVPD, EID and ECD in an automated fashion during an LCMS type experiment. We exploited this...

I am really pleased we managed to squeeze this story out during #ASMS2025. This is our contribution relating to the fantastic Omnitrap/Orbitrap. What we thought would be a straightforward conversation of our exploris-omnitrap turned into quite a journey. (1/n)

www.biorxiv.org/cgi/content/...

🚨🚨 News from the @castello-lab.bsky.social and @shabazlab.bsky.social. Our work, led by Louisa Iselin, reveals pervasive changes in the RNA-bound proteome induced by interferon. #RNA, #immunity, #interferon, #host-virus www.biorxiv.org/content/10.1...

RNA binding regulation is a new dimension in the type I IFN response The type I interferon (IFN-I) response shapes the intracellular environment to supress virus infection. Historically, this remodelling has been linked to the transcriptional induction of interferon st...

Proud of Louisa Iselin for this work. Not only a covid baby but had both of her supervisors move lab (@alf-castello.bsky.social and me). The works is a meticulous analysis of the interferon pathway and specifially how RNA binding of proteins are modulated by PTMs.

www.biorxiv.org/content/10.1...

Happy to see Honglin Chen's paper published! 🎉🎉🍾🥂. Great collaboration with @shabazlab.bsky.social to discover the discordances between RNA and protein in the interferon response...

Addressing NHS chemistry: Efficient quenching of excess TMT reagent and reversing TMT-over labelling in proteomic samples by methylamine N-Hydroxysuccinimide (NHS) ester chemistry is used extensively across proteomics sample preparation. One of its increasingly prevalent applications is in isobaric reagent-based quantitation such as iT...

A basic solution to a very common problem.

collab with @kusterlab.bsky.social and @bendavisgroup.bsky.social

www.mcponline.org/article/S153...

Great conference at @embl.org on the expanding world of #RBPs. After the hard (fun) work, the @castello-lab.bsky.social chilled out in the beautiful Heidelberg

Pre-print is up! SARS-2 produces a truncated N protein to antagonize multiple antiviral pathways. A very collaborative project in the @corcoranlab.bsky.social with @nogasharlin.bsky.social, Danyel Evseev, and Max Bui-Marinos, and of course the boss Jenn Corcoran

www.biorxiv.org/content/10.1...

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/...
#RNAsky #RNAbiology #Cryo-EM

Excellent talk of Louisa Iselin on the remodelling of the RBPome by interferon at the "Expanding Universe of RBPs" in @embl.org, Heidelberg. The @castello-lab.bsky.social is very proud 🍾🥂🎊🎊

Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs - Communications Chemistry Translation of mRNA into protein is a fundamental process and tightly controlled during development, however, experimentally controlling the mRNA translation remains challenging. Here, the authors dem...

Translation of mRNA can be triggered with spatiotemporal control in zebrafish 🐟 embryos by light ⚡ & FlashCaps 🧪⚗😊 Kudos to all authors and collaborators 🙏👍
www.nature.com/articles/s42...

Imp/IGF2BP and Syp/SYNCRIP temporal RNA interactomes uncover combinatorial networks of regulators of Drosophila brain development RNA binding protein combinations control neuronal identity through a network of temporal regulators.

Amazing work of our dear friends @ilandavis.bsky.social and @jeffylee.bsky.social characterising the interlinked roles of two key RBPs in drosophila brain development, congratulations 🍾🥂🎉🎉🎉 www.science.org/doi/10.1126/...

Had a wonderful couple of days with these guys. @alf-castello.bsky.social @marvintanenbaum.bsky.social and Stephen carter. Always a pleasure

At this END of YEAR please check out our new paper on GENE ENDS and a convenient marker of transcription termination: RNAPII CTD T4ph! We think it is relevant to anyone studying human/animal #genes and #genome so put a lot of effort to make it easy to read. #RNABiology and #Epigenetics meet:)

Why Bax detection in >1400 publications might be flawed - Cell Death & Disease Cell Death & Disease - Why Bax detection in >1400 publications might be flawed

These studies are so important. Well done to the authors and the journal for publishing it. This certainly applies to so many antibodies. Companies selling antibodies should do and provide the right controls before commercialisation.

www.nature.com/articles/s41...