Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates and Exposes Selective Neuronal Vulnerability The ER's continuous tubular network is maintained by ER-shaping proteins whose mutation or dysregulation contributes to neurodegenerative diseases. Here, we show that ER morphology sets the speed of ...

🚨📜Our new paper out in @AdvSciNews:
ER shape matters for neuronal health - disrupting ER continuity halts neuro-firing as it slows Ca2+ store refill, explaining selective neuronal vulnerability to ER problems in neurodegeneration 🧠
a thread to follow advanced.onlinelibrary.wiley.com/doi/10.1002/...

I've been leading a big project at Methods in Enzymology over the past year or so: editing three back-to-back-to-back volumes of articles describing the latest advances in methods for studying lipids and membranes — thank you to the 50 #lipidtime luminaries who contributed the 51 chapters! Details 👇

Curve Appeal: The Architecture and Engineering of Membrane Bending This webinar, hosted by the Membrane Structure & Function Subgroup of the Biophysical Society, and organized by Adam Smith, will highlight how lipid composition, protein assemblies, and membrane mechanics work together to generate and regulate membrane curvature in cells. Three invited speakers will present on membrane bending in a way that's accessible to undergraduate students interested in biochemistry and biophysics, with sufficiently many fun new things sprinkled in to also be interesting to specialists in the field. Invited speakers are: Itay Budin (UC San Diego) Margaret E. Johnson (Johns Hopkins University) Cecília Leal (University of Illinois Urbana–Champaign). Sign-up link for the webinar: https://my.biophysics.org/Events/Calendar-Of-Events/Meeting-Home-Page?meetingid=95b51c20-8219-f111-8342-7ced8d22117d We hope to have as lively an event as we had a year ago, when we also hosted a biophysics membrane webinar! Log in—it'll be fun! Please share widely!

📣 To celebrate Biophysics Week, the Membrane Structure and Function Subgroup (MSAF) of @biophysicalsoc.bsky.social will host a webinar on March 24 at 11am EDT. Our speakers are @mejohnson81.bsky.social, @ibudin.bsky.social, and Cecília Leal (not on 🦋). 🧪

👀 More info in the alt text! Please share! 🔄

Meeting Home Page Displays details of a selected meeting including Date, Time, location, and additional content.

Curve Appeal: The Architecture and Engineering of Membrane Bending
-Membrane Structure & Function Subgroup-
March 24, 11AM-12PM

Learn how lipid composition, protein assemblies &membrane mechanics work together to generate & regulate membrane curvature in cells.

Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis - Nature Structural & Molecular Biology Here, Deol et al. use genetic screens in gene-edited reporter cell lines to identify regulators of ferroptosis suppressor protein 1 (FSP1) expression and stability. They show that vitamin B2 metabolis...

1/3 Thrilled to see our paper led by Kirandeep Deol is out in Nature Structural & Molecular Biology! CRISPR screens to uncover a role for vitamin B2 metabolism in stabilizing FSP1 via FAD, revealing a new way vitamins regulate ferroptosis.
www.nature.com/articles/s41...

Happy our lab could contribute to this exciting story from the Thomas Becker and Nikolaus Pfanner labs! A protein #Translocase forms #LipidDroplet #Mitochondria #ContactSites via a substrate-mimicry mechanism! www.nature.com/articles/s41...

Congrats!!!

Happy to share our new paper in @febsj.bsky.social! With
@ondrej-kuda.bsky.social & @fedorovalab.bsky.social we show how #LipidDroplets reshape the lipidome of PUFA-exposed cells, but can also shift function within the same cell, revealing a context-dependent role in #ferroptosis. shorturl.at/TyRl1

Congratulations 🎉🎉🎉🎉 @olzmannlab.bsky.social @arrudalab.bsky.social

CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly - Nature CRISPR–Cas9 screening identifies CLCC1 as a factor that increases neutral lipid flux to prevent hepatic steatosis and promotes nuclear pore complex assembly by promoting membrane bending and fusi...

Sooo happy to share our new paper in @nature.com “CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.” A terrific collaboration with @arrudalab.bsky.social, led by co–first authors Alyssa Mathiowetz and Emily Maymand.
www.nature.com/articles/s41...

Kudos to Tobias ! Warm greetings to my MSF freinds, sad I am missing it this year.

#VAMP7-dependent late endosomal #secretion of #ER and #mitochondrial proteins impacts the #tumor #microenvironment and #macrophage engagement
www.nature.com/articles/s41...

📢 Registration for the 28th Annual Meeting of the Club Exocytose-Endocytose is open until April 15 !
👉 Register here: exoendo.org/en/evenement...
Please share widely!
@gdrimabio.bsky.social @biologists.bsky.social @sbcf.bsky.social @embo.org @febsletters.bsky.social @sneuroendo.bsky.social

A model to describe what we show in the paper. INVs are important for sequestering GLUT4 and related proteins into GSVs, the vesicles that are mobilised in response to insulin.

🚨 We have a new preprint out! 🧪

In this paper we look at GLUT4 trafficking. This is a short explainer thread, but please read the paper!

1/n 🧵

www.biorxiv.org/content/10.6...

À M À Z I N G

Cells come in many shapes and sizes, with diverse physiological functions. But how do #organelles and their interaction networks remodel during #differentiation of stem cells into different cell types? Here’s what we discovered about neuronal differentiation: 1/13

Check out our newest findings on how #lysosomes are generated from #endolysosomes. Great work from @samit2104.bsky.social ! @biozentrum.unibas.ch , @unibas.ch #cellbio #cell #organelles
Ca2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites www.biorxiv.org/content/10.6...

Structure of the adaptor protein 4 (AP-4) complex revealed! AP-4 exists in a dynamic equilibrium between closed and open conformations in solution. ARF1 does not shift this equilibrium but is required—together with cargo—for membrane recruitment. rdcu.be/e1ozM

Huge congrats to @manuelthery.bsky.social and all the Cytomorpho lab. The "Living Architectures" performance at @museeorsay.bsky.social was breathtakingly beautiful

Thanks!!

I’ll soon share a call for a postdoc at Institut Jacques Monod (Paris) on fundamental mechanisms of membrane traffic and protein/collagen secretion in health and disease. Please get in touch if interested.

New preprint from the lab! Triglycerides act like salt on ice: they solubilize cholesterol esters and drive lipid droplet growth. The TG/CE ratio shapes both droplet size and proteome, mirroring lipoprotein organization.

www.biorxiv.org/content/10.6...

Triglyceride/Cholesterol Ester Ratio Encodes Lipid Droplet Size and Diversity www.biorxiv.org/content/10.64898/2026.01...

The work by @danialv.bsky.social is now out in @pnas.org with a few changes after peer-review. Have a look if you are interested in lipid transport by bridge lipid transfer proteins (BLTPs)...
www.pnas.org/doi/10.1073/...

Guillaume Pernollet shows that epithelial cells adjust their shape to locally flatten, forming scutoids for any geometry, changing our view of cell packing. Thanks to all! @clairedessalles.bsky.social @juanmagararc.bsky.social @sciencesunige.bsky.social
www.biorxiv.org/content/10.6...

LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.

🔗 doi.org/10.64898/202...

#CryoET

Such a pleasure to write this review on #LipidDroplet heterogeneity, dynamics and #organelle interactions with Mike @hennelab.bsky.social ! Thank you to the editorial team @natrevmcb.nature.com and to Yoni Malis for assistance with draft illustrations

Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7)
www.biorxiv.org/content/10.6...

Membrane editing with proximity labeling reveals regulators of lipid homeostasis - Nature Chemical Biology Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.

Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇

Registration is open for the FASEB #LipidDroplet meeting in Scottsdale, July 26-30! This is THE premier North American conference on LDs, you do not want to miss it!