Complete biosynthesis of psychedelic tryptamines from three kingdoms in plants Tryptamine psychedelics from plants, fungi, and toads are produced in planta through cross-kingdom pathway reconstruction.

www.science.org/doi/10.1126/...
#agroinfiltration
#pharmaceutical-farming
#psychedelics
Psilocin & psilocybin (mushrooms), DMT (various plants), bufotenin & 5-methoxy-DMT (toads) manufactured in easy-to-cultivate plants (such as tobacco) by alteration of a handful of genes.

Transient gene expression in petunia flower petals as a toolbox Agroinfiltration-mediated transient expression is widely used in plant gene functional studies. In this study, the functionality of a metabolic engineering construct containing multiple expression cassettes for betalain biosynthesis genes was validated via agroinfiltration in petunia flower petals.

#Agroinfiltration of #petunia #flower petals as a rapid, easily scalable, and highly reproducible toolbox for #gene functional characterisation.
To rediscover one of our most read articles in JHSB 99:2, visit: doi.org/10.1080/1462...

@chenxinli2.bsky.social

Agroinfiltration - Wikipedia

TIL:

en.wikipedia.org/wiki/Agroinf...

" #Agroinfiltration is a method used in plant biology and especially lately in plant biotechnology to induce #TransientExpression of genes in a plant, or isolated leaves from a plant, or even in cultures of plant cells, in order to produce a desired protein."

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana Agroinfiltration of Nicotiana benthamiana is routinely used for transient gene expression in plant sciences and molecular pharming. Here, we depleted transcripts of 21 different immunity-related genes through virus-induced gene silencing (VIGS) to identify genes that hamper transient expression in juvenile plants. These experiments uncovered that silencing of ethylene insensitive-2 (EIN2), receptor-like kinase CERK1, transcription factor regulator nonexpressor of pathogenesis-related genes 1 (NPR1) and isochorismate synthase (ICS), increases transient GFP expression by 2-, 4-, 4- and 11-fold, respectively. Accordingly, the npr1a/npr1b double mutant of N. benthamiana does indeed facilitate increased protein accumulation when transiently expressed. These results indicate that glycan perception through CERK1, and ET and SA signaling pathways via EIN2 and NPR1/ICS, respectively, contribute to suppressed transient gene expression in frequently used juvenile N. benthamiana plants. ### Competing Interest Statement The authors have declared no competing interest.

Thrilled to see this out!

Silencing immunity genes boosts transient expression in Nicotiana benthamiana! 🌱🧬
🔗 doi.org/10.1101/2025...

Congrats to Issy and everyone involved! #PlantChemeticsLab #Agroinfiltration #TransientExpression #MolecularFarming

Improving transient protein expression in agroinfiltrated Nicotiana benthamiana <em>New Phytologist</em> is an international journal owned by the New Phytologist Foundation publishing original research in plant science and its applications.

📜 Improving transient protein expression in agroinfiltrated Nicotiana benthamiana

🧑‍🔬 Konstantina Beritza, Emma C. Watts, Renier A. L. van der Hoorn

📔 @newphyt.bsky.social

🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...

#️⃣ #PlantScience #PlantMethods #Agroinfiltration #PlantTransformation