Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen Pseudomonas syringae pv. tomato DC3000 produces ...

Pseudomonas syringae secretes the iminosugar glycosyrin to block plant immune recognition. It mimics galactose to inhibit β-galactosidase, disrupt flagellin sensing, and rewire host glycobiology!
doi.org/10.1126/scie...

Huge congrats to Tee and everyone involved!
#PlantImmunity #PlantChemeticsLab

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana Agroinfiltration of Nicotiana benthamiana is routinely used for transient gene expression in plant sciences and molecular pharming. Here, we depleted transcripts of 21 different immunity-related genes through virus-induced gene silencing (VIGS) to identify genes that hamper transient expression in juvenile plants. These experiments uncovered that silencing of ethylene insensitive-2 (EIN2), receptor-like kinase CERK1, transcription factor regulator nonexpressor of pathogenesis-related genes 1 (NPR1) and isochorismate synthase (ICS), increases transient GFP expression by 2-, 4-, 4- and 11-fold, respectively. Accordingly, the npr1a/npr1b double mutant of N. benthamiana does indeed facilitate increased protein accumulation when transiently expressed. These results indicate that glycan perception through CERK1, and ET and SA signaling pathways via EIN2 and NPR1/ICS, respectively, contribute to suppressed transient gene expression in frequently used juvenile N. benthamiana plants. ### Competing Interest Statement The authors have declared no competing interest.

Thrilled to see this out!

Silencing immunity genes boosts transient expression in Nicotiana benthamiana! 🌱🧬
🔗 doi.org/10.1101/2025...

Congrats to Issy and everyone involved! #PlantChemeticsLab #Agroinfiltration #TransientExpression #MolecularFarming

Amazing to see this beautiful work in its final form!
Many congratulations @pierrebuscaill.bsky.social et al!
#PlantChemeticsLab #Proteases

Subtilase SBT5.2 inactivates flagellin immunogenicity in the plant apoplast - Nature Communications Plants recognize bacteria by perceiving a 22-residue epitope in flagellin. Plant-secreted SBT5.2 subtilases are found to inactivate this epitope, leading to elicitor removal and reducing costly immuni...

🌿 Excited to share our latest research on how host proteases regulate immune activation!

Read more about how SBT5.2 releases and inactivates flg22: www.nature.com/articles/s41...
rdcu.be/d1UsT

Congratulations to all involved!
#PlantImmunity #PlantPathology #PlantChemeticsLab