#FruitRes
Optimized Agrobacterium-mediated transient gene expression in citrus shows six-fold increase in GUS expression. Method aids HLB research and genetic studies. 🌳

#Citrus #TransientExpression #HLB
Details: maxapress.com/article/doi/...

Agroinfiltration - Wikipedia

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en.wikipedia.org/wiki/Agroinf...

" #Agroinfiltration is a method used in plant biology and especially lately in plant biotechnology to induce #TransientExpression of genes in a plant, or isolated leaves from a plant, or even in cultures of plant cells, in order to produce a desired protein."

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana Click on the article title to read more.

Thrilled to see this out! 🎉 doi.org/10.1111/pbi....

🌿🧬 Silencing immunity genes boosts transient expression in Nicotiana benthamiana!

Congrats to all involved! A #PlantChemetics story first on @biorxivpreprint.bsky.social, now in @PlantBiotechJ.

#TransientExpression #MolecularPharming

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana Agroinfiltration of Nicotiana benthamiana is routinely used for transient gene expression in plant sciences and molecular pharming. Here, we depleted transcripts of 21 different immunity-related genes through virus-induced gene silencing (VIGS) to identify genes that hamper transient expression in juvenile plants. These experiments uncovered that silencing of ethylene insensitive-2 (EIN2), receptor-like kinase CERK1, transcription factor regulator nonexpressor of pathogenesis-related genes 1 (NPR1) and isochorismate synthase (ICS), increases transient GFP expression by 2-, 4-, 4- and 11-fold, respectively. Accordingly, the npr1a/npr1b double mutant of N. benthamiana does indeed facilitate increased protein accumulation when transiently expressed. These results indicate that glycan perception through CERK1, and ET and SA signaling pathways via EIN2 and NPR1/ICS, respectively, contribute to suppressed transient gene expression in frequently used juvenile N. benthamiana plants. ### Competing Interest Statement The authors have declared no competing interest.

Thrilled to see this out!

Silencing immunity genes boosts transient expression in Nicotiana benthamiana! 🌱🧬
🔗 doi.org/10.1101/2025...

Congrats to Issy and everyone involved! #PlantChemeticsLab #Agroinfiltration #TransientExpression #MolecularFarming