N*M210 inhibits SG/RLB formation by sequestering dsRNA. HeLa cells expressing N*M210-FLAG (blue) were transfected with poly (I:C) (pink) and SG/RLB formation was measured using TIAR (yellow). This experiment was conducted and imaged by Rory P Mulloy, who agrees to publish this image under a CC-BY licence.

#SARSCoV2 has optimized its replication fitness in the human host. @rorymulloy.bsky.social @corcoranlab.bsky.social &co show that a #nucleocapsid gene mutation enhances production of a truncated protein that boosts viral fitness by suppressing #antiviral responses @plosbiology.org 🧪 plos.io/3PIZMjr

N*M210 inhibits SG/RLB formation by sequestering dsRNA. HeLa cells expressing N*M210-FLAG (blue) were transfected with poly (I:C) (pink) and SG/RLB formation was measured using TIAR (yellow). This experiment was conducted and imaged by Rory P Mulloy, who agrees to publish this image under a CC-BY licence.

#SARSCoV2 has optimized its replication fitness in the human host. @rorymulloy.bsky.social @corcoranlab.bsky.social &co show that a #nucleocapsid gene mutation enhances production of a truncated protein that boosts viral fitness by suppressing #antiviral responses @plosbiology.org 🧪 plos.io/3PIZMjr

N*M210 inhibits SG/RLB formation by sequestering dsRNA. HeLa cells expressing N*M210-FLAG (blue) were transfected with poly (I:C) (pink) and SG/RLB formation was measured using TIAR (yellow). This experiment was conducted and imaged by Rory P Mulloy, who agrees to publish this image under a CC-BY licence.

#SARSCoV2 has optimized its replication fitness in the human host. @rorymulloy.bsky.social @corcoranlab.bsky.social &co show that a #nucleocapsid gene mutation enhances production of a truncated protein that boosts viral fitness by suppressing #antiviral responses @plosbiology.org 🧪 plos.io/3PIZMjr

#Medsky🧪 #IDsky #Neurosky #publichealth
#SARSCoV2 #nucleocapsid (N) protein induces microglia senescence
& associated cognitive impairment by causing mitochondrial dysfunction which forces a metabolic shift ➡️ #glycolysis. The glycolytic reprogramming triggers the activation of cellular senescence.

higher IgG avidity for the full #SARSCoV2 spike protein and the #Nucleocapsid protein vs to adults. ie 56% of children had high-avidity antibodies, whereas only 22.8% of adults did. This suggests that children generated a targeted, high-avidity

ACROBiosystems SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag NUNC51H91G - GenomiRX ACROBiosystems SARS-CoV-2 (COVID-19) Nucleocapsid protein

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ACROBiosystems SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag NUNC51H91G @Acrobio #sars #cov2 #covid19 #Nucleocapsid #protein #NUNC51H91G

📣 New Method

An efficient strategy for producing RNA-free Nucleocapsid protein of SARS-CoV-2 for biochemical and structural investigations

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🖊️ @TheGuptaLab

#SARSCoV2 #Nucleocapsid

🧬 How do SARS-CoV-2 mutations boost fitness?

With ISIDORe, Blackledge (IBS Grenoble) used high-field NMR to show that N protein mutations (e.g. Q229K, R203M) reshape structure & RNA binding.

Disorder = function.

#ISIDORe #SARSCoV2 #Nucleocapsid #NMR #ViralFitness #ProteinDisorder

N:G215C virions are enlarged and show over-incorporation of N protein. Vero-TMPRSS2 cells were infected with WT (left) or N:G215C (right) viruses at an MOI of 0.1. The following day cells were prepared for electron microscopy by high-pressure freezing and freeze-substitution, then sectioned and imaged by dual-axis electron tomography. Virus-containing exit compartments were located in both samples, and virions that had completely separated from cellular membranes were selected and analyzed in 3D in order to determine the structure of intact virions and the arrangement of internal ribonucleoprotein complexes.

Non-spike protein mutations in #SARSCoV2, likely to impact viral properties, are understudied. @brucelab.bsky.social show that a #nucleocapsid protein mutation in Delta variant enhances viral growth in vitro & in vivo by increasing viral genome #encapsidation @plosbiology.org 🧪 plos.io/4iBYsaI

N:G215C virions are enlarged and show over-incorporation of N protein. Vero-TMPRSS2 cells were infected with WT (left) or N:G215C (right) viruses at an MOI of 0.1. The following day cells were prepared for electron microscopy by high-pressure freezing and freeze-substitution, then sectioned and imaged by dual-axis electron tomography. Virus-containing exit compartments were located in both samples, and virions that had completely separated from cellular membranes were selected and analyzed in 3D in order to determine the structure of intact virions and the arrangement of internal ribonucleoprotein complexes.

Non-spike protein mutations in #SARSCoV2, likely to impact viral properties, are understudied. @brucelab.bsky.social show that a #nucleocapsid protein mutation in Delta variant enhances viral growth in vitro & in vivo by increasing viral genome #encapsidation @plosbiology.org 🧪 plos.io/4iBYsaI

N:G215C virions are enlarged and show over-incorporation of N protein. Vero-TMPRSS2 cells were infected with WT (left) or N:G215C (right) viruses at an MOI of 0.1. The following day cells were prepared for electron microscopy by high-pressure freezing and freeze-substitution, then sectioned and imaged by dual-axis electron tomography. Virus-containing exit compartments were located in both samples, and virions that had completely separated from cellular membranes were selected and analyzed in 3D in order to determine the structure of intact virions and the arrangement of internal ribonucleoprotein complexes.

Non-spike protein mutations in #SARSCoV2, likely to impact viral properties, are understudied. @brucelab.bsky.social show that a #nucleocapsid protein mutation in Delta variant enhances viral growth in vitro & in vivo by increasing viral genome #encapsidation @plosbiology.org 🧪 plos.io/4iBYsaI

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Intrinsic factors in long- #COVID
www.researchgate.net/post/The-nov... ..
1 Prevalence of the #extracellular #vesicles
2 #SARSCoV2, extracellular vesicles, and #neurological #disorders.
3 Persistence of #SARS CoV-2 and its components
4 .. #nucleocapsid #protein ..
5 #Immunometabolic Disorders