#SexDet

Title slide of the presentation, with Stevens' plates on the left and text on the right. The title is "Beyond sex chromosomes: how sexual processes shape genomes and molecules". Details about my current affiliation and the seminar are also given.

NM Stevens portrait

Just gave a seminar on the #genomics of #SexDet for students of the uni of Bologna.

I included these wonderful plates by Nettie M Stevens, the scientist who first linked XY chromosomes to SexDet: she was a woman and, guess what, she is rarely acknowledged for her work

#EndViolenceAgainstWomen

I am a free (as in freedom) and open source software advocate, formerly actively involved in FOSS groups 🕊️
I love dataviz, photography, classic rock music, playing golf and video games 🤘⛳
#introduction #stem #DevBio #genetics #genomics #transcriptomics #SexDet 2/2

Hey there!

I'm a French researcher in developmental biology, focusing on mammalian sex determination and gonadal development at the genetic level. I have a PhD in bioinformatics but I do wet and dry lab 👩‍🔬🔬👩‍💻
#introduction #stem #DevBio #genetics #genomics #transcriptomics #SexDet 1/2

Divergent regulatory element programs steer sex-specific supporting cell differentiation along mouse gonadal development Gonadal sex determination relies on tipping a delicate balance involving the activation and repression of several transcription factors and signalling pathways. This is likely mediated by numerous non-coding regulatory elements that shape sex-specific transcriptomic programs. To explore the dynamics of these in detail, we performed paired time-series of transcriptomic and chromatin accessibility assays on pre-granulosa and Sertoli cells throughout their development in the embryo, making use of new and existing mouse reporter lines. Regulatory elements were associated with their putative target genes by linkage analysis, and this was complemented and verified experimentally using promoter capture Hi-C. We identified the transcription factor motifs enriched in these regulatory elements along with their occupancy, pinpointing LHX9/EMX2 as potentially critical regulators of ovarian development. Variations in the DNA sequence of these regulatory elements are likely to be responsible for many of the unexplained cases of individuals with Differences of Sex Development. ### Competing Interest Statement The authors have declared no competing interest.

📣 Preprint Alert ! We just released the manuscript of our study of the regulatory elements that control fetal testis and ovary development in mice! www.biorxiv.org/content/10.1101/2024.12....
#sexdet #DevBio #bioinformatics #RStats