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Exciting new updates in @PomBase.bsky.social, curated by the #fissionyeast Schizosaccharomyces pombe community, showcase high-quality, detailed annotations of gene functions that cover around 80% of the proteome.

Learn more in #GENETICS about this curation project: buff.ly/FPyuwCg

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#Telomerase #RNA #Structure #Spombe #FissionYeast

S. pombe telomerase RNA: secondary structure and flexible-scaffold function

Karen McMurdie, Allison N. Peeney, Melissa A. Mefford, Peter Baumann, David C. Zappulla

www.biorxiv.org/content/10.1...

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(Top) Schematic representation of Fus1opto used to block cells pre-fusion in the dark and synchronize fusion upon blue light exposure. (Bottom) Time-lapse movie of h90 fus1opto cells mated in the dark and exposed to blue light at t = 0’, where Fus1C-CRY2 is tagged with sfGFP and cytosolic mTagBFP2 is expressed from the P-cell specific map3 promoter.

(Top) Schematic representation of Fus1opto used to block cells pre-fusion in the dark and synchronize fusion upon blue light exposure. (Bottom) Time-lapse movie of h90 fus1opto cells mated in the dark and exposed to blue light at t = 0’, where Fus1C-CRY2 is tagged with sfGFP and cytosolic mTagBFP2 is expressed from the P-cell specific map3 promoter.

Nitrogen-starvation of #FissionYeast inactivates growth pathways (incl TORC1) & can trigger sexual reproduction. @sophiemartinlab.bsky.social use proteomic & phosphoproteomic changes during sexual development to show that TORC1 is reactivated by pheromone signaling 🧪 @plosbiology.org plos.io/4h3OUVO

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(Top) Schematic representation of Fus1opto used to block cells pre-fusion in the dark and synchronize fusion upon blue light exposure. (Bottom) Time-lapse movie of h90 fus1opto cells mated in the dark and exposed to blue light at t = 0’, where Fus1C-CRY2 is tagged with sfGFP and cytosolic mTagBFP2 is expressed from the P-cell specific map3 promoter.

(Top) Schematic representation of Fus1opto used to block cells pre-fusion in the dark and synchronize fusion upon blue light exposure. (Bottom) Time-lapse movie of h90 fus1opto cells mated in the dark and exposed to blue light at t = 0’, where Fus1C-CRY2 is tagged with sfGFP and cytosolic mTagBFP2 is expressed from the P-cell specific map3 promoter.

Nitrogen-starvation of #FissionYeast inactivates growth pathways (incl TORC1) & can trigger sexual reproduction. @sophiemartinlab.bsky.social use proteomic & phosphoproteomic changes during sexual development to show that TORC1 is reactivated by pheromone signaling 🧪 @plosbiology.org plos.io/4h3OUVO

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(Top) Schematic representation of Fus1opto used to block cells pre-fusion in the dark and synchronize fusion upon blue light exposure. (Bottom) Time-lapse movie of h90 fus1opto cells mated in the dark and exposed to blue light at t = 0’, where Fus1C-CRY2 is tagged with sfGFP and cytosolic mTagBFP2 is expressed from the P-cell specific map3 promoter.

(Top) Schematic representation of Fus1opto used to block cells pre-fusion in the dark and synchronize fusion upon blue light exposure. (Bottom) Time-lapse movie of h90 fus1opto cells mated in the dark and exposed to blue light at t = 0’, where Fus1C-CRY2 is tagged with sfGFP and cytosolic mTagBFP2 is expressed from the P-cell specific map3 promoter.

Nitrogen-starvation of #FissionYeast inactivates growth pathways (incl TORC1) & can trigger sexual reproduction. @sophiemartinlab.bsky.social use proteomic & phosphoproteomic changes during sexual development to show that TORC1 is reactivated by pheromone signaling @plosbiology.org plos.io/4h3OUVO

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Original printed figure for a scientific paper published in 1989.

Original printed figure for a scientific paper published in 1989.

From the archives: the original 1989 artwork for Figure 3 in PMID:2674650 pubmed.ncbi.nlm.nih.gov/2674650/ #pombe #fissionyeast #cellcycle #cdc2

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A Petri dish with agar growth medium has fission yeast colonies from tetrads. Tetrad colonies are aligned vertically.

A Petri dish with agar growth medium has fission yeast colonies from tetrads. Tetrad colonies are aligned vertically.

A Petri dish with agar growth medium has patches of fission yeast that are white or pink.

A Petri dish with agar growth medium has patches of fission yeast that are white or pink.

Belated introduction after more than a year on Bluesky 😊
While I post a lot about #Nature, #River, #FlyFishing and #IPA, I use the fission yeast Schizosaccharomyces pombe for my research. At some point, I plan to brew #Beer🍺

#FissionYeast #Pombe #Science #SchizosaccharomycesPombe

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An alignment of patches of fission yeast growth on an agar medium on a Petri dish. 

Some show pink, some are white.

An alignment of patches of fission yeast growth on an agar medium on a Petri dish. Some show pink, some are white.

Got some pink yeast 😊
Can we make pink beer if we use this yeast strain ??? 🤔

The ade6 gene mutation causing red pigments for this color change in the fission yeast S. pombe 😉

#Pombe #FissionYeast #Yeast #Science #Beer #Pink

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S. Pombe tetrads from the parental asci are order aligned and have formed colonies on agar growth medium in a petri dish. There are some missing colonies when spores are not viable to grow and proliferate.

S. Pombe tetrads from the parental asci are order aligned and have formed colonies on agar growth medium in a petri dish. There are some missing colonies when spores are not viable to grow and proliferate.

#TetradTuesday

Did some tetrad dissection of fission yeast (S. pombe) spores on Friday 😊
Today, they are showing pretty colonies 🥳

Tetrad = four spores produced after yeast meiosis

#TetradDissection #Tetrad #Spore #Yeast #FissionYeast #Science #Pombe

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