An 8-nm precise map in live cells, which revealed #actin aster formation by #myosin V-driven transport of #formin.
Happy to see this work now out! 😃
In collaboration with @kaksonen.bsky.social lab
#yeast #pombe #cell_fusion
@biology-unige.bsky.social
In @jcb.org, @valthomas.bsky.social et al. @sophiemartinlab.bsky.social provide a live, 8-nm precision map of the actin fusion focus for cell-cell fusion in S. #pombe. This reveals #actin aster formation by #myosin Myo52-driven transport of the formin Fus1 nucleator rupress.org/jcb/article/...
New work by @valthomas.bsky.social et al. in @sophiemartinlab.bsky.social provides a live, 8-nm precision map of the actin fusion focus for cell-cell fusion in S. #pombe. This reveals #actin aster formation by #myosin Myo52-driven transport of the formin Fus1 nucleator rupress.org/jcb/article/...
My former student Josh just had his paper accepted at #Genetics showing that #pombe diploids have intrinsic #genomeinstability . Be identified ploidy-essential genes and a remarkable loss of heterozygosity phenotype that may identify specific fragile sites.
🧬
@genetics-gsa.bsky.social
First post here, my new review on how #calcium regulates #cytokinesis. It recounts discovery of cytokinetic calcium spikes in #pombe and the force-sensing channel driving such spikes. I compare #yeast to animal cells on calcium transients. Lots of unknown about these mysterious calcium currents.
Biotin, pantothenic acid, nicotinic acid, and inositol near a scale.
My pombe (fission yeast) didn’t mate 😌
Preparing a different medium for mating, giving them some B vitamins 😉
Yeast needs vitamins, I also need vitamins 🤔
#Pombe #Yeast #Science #Experiment #Vitamins #Health
A PVDF membrane with protein bands detected by antibodies and chemiluminescent substrate.
A beautiful Western blot that I did with new undergraduate students 🥳
S. pombe nmt1 (81) promoter worked well to repress gene expression 🧐
#Science #WesterBlotting #Protein #Pombe #Promoter
Cloudy golden IPA in a pint glass with its can on the side. Iron Hill Brewery, Philly Phavorite IPA.
I spent the day running experiments with yeast (S. pombe) instead of sitting in front of a computer 😊
I wondered how much yeast (S. cerevisiae) is in this cloudy IPA, looks like way more than I used in my lab today 🤔
#Beer #IPA #Yeast #Pombe #Science #Photography
Eight test tubes with yeast culture on a green rack. Seven cultures have flocculation, while three don’t.
#Flocculation#Friday
A few S. pombe strains don’t flocculate 🤔
My favorite mutant doesn’t seem to be great for brewing beer 🤔
My research doesn’t have anything to do with brewing, though 🤔
#Yeast #Pombe #Beer #Science #Philadelphia #Flocculation
#pombe ❤️
Nice😊
I plan to do some serial dilution growth assays (aka spot assay on frogging) for S. pombe strains on Friday 😉
#Yeast #Pombe
Three stacks of Petri dishes containing agar growth medium sit on the black research bench, with bottles and tubes of buffer and chemicals and a green tube rack in the background.
Tomorrow is the deadline, the office staff told me that the NIH grant application system has been a bit unstable, my brain is also a bit unstable because of sleep deprivation, so I made some agar plates to clear my mind, it seemed to help 🤔
#Proposal #Science #MentalHealth #Writing #Yeast #Pombe
An open 96-well plate, a few closed Petri dishes, an 8-channel pipette are in a black research bench. A metal 48-pin replicator is sitting on the 96-well plate.
#ThursdayThoughts
Running last-minute experiments for my proposal, a student in a neighboring lab said never seen me “doing science” before. Sure he meant “doing experiments,” but I do feel “doing science” when I'm doing experiments, rather than working (on #Science) at a computer🤔
#Yeast #Pombe
The new POMBOX kit is now available from @addgene.bsky.social
To plan your cloning with this and other Golden Gate kits, you can try the kit template in #ShareYourCloning
🔗 Visit shareyourcloning.org
👉 Go to Templates > Moclo
🎞️ Full demo video www.youtube.com/watch?v=n0he...
#pombe #MoClo
Original printed figure for a scientific paper published in 1989.
From the archives: the original 1989 artwork for Figure 3 in PMID:2674650 pubmed.ncbi.nlm.nih.gov/2674650/ #pombe #fissionyeast #cellcycle #cdc2
A Petri dish with agar growth medium has fission yeast colonies from tetrads. Tetrad colonies are aligned vertically.
A Petri dish with agar growth medium has patches of fission yeast that are white or pink.
Belated introduction after more than a year on Bluesky 😊
While I post a lot about #Nature, #River, #FlyFishing and #IPA, I use the fission yeast Schizosaccharomyces pombe for my research. At some point, I plan to brew #Beer🍺
#FissionYeast #Pombe #Science #SchizosaccharomycesPombe
Very nice to see my recent book chapter on meiotic recombination assays in Schizosaccharomyces #pombe out today doi.org/10.1007/978-...
An alignment of patches of fission yeast growth on an agar medium on a Petri dish. Some show pink, some are white.
Got some pink yeast 😊
Can we make pink beer if we use this yeast strain ??? 🤔
The ade6 gene mutation causing red pigments for this color change in the fission yeast S. pombe 😉
#Pombe #FissionYeast #Yeast #Science #Beer #Pink
S. Pombe tetrads from the parental asci are order aligned and have formed colonies on agar growth medium in a petri dish. There are some missing colonies when spores are not viable to grow and proliferate.
#TetradTuesday
Did some tetrad dissection of fission yeast (S. pombe) spores on Friday 😊
Today, they are showing pretty colonies 🥳
Tetrad = four spores produced after yeast meiosis
#TetradDissection #Tetrad #Spore #Yeast #FissionYeast #Science #Pombe
A microscope with a micro manipulator that has a needle to dissect yeast tetrads. There is a Petri dish with agar growth medium on the stage. The Petri dish is placed upside down, so that yeast spore can be manipulated from the bottom by the needle.
Did some yeast (S. pombe) tetrad dissection 😉
It’s pretty meditating on Friday night after Happy Hour ☺️
Hoping to get some nice results 😊
#Yeast #Pombe #Tetrad #Science #Genetics #Meditation #HappyHour
Two stacks of Petri dishes with agar growth medium are on a black-top research station. A flask and its cap are on the side.
Some agar growth medium for fission yeast mating😊
They have two mating types: h- and h+ 😉
Malt extract in the medium 😊
Fission yeast - Schizosaccharomyces pombe 😉
I just made a successful pollination for our pumpkin (I think), now fission yeast 😊
#Experiment #Science #Pombe #Yeast #Agar #Mating
Two stacks of plates are incubating in an incubator with a silver lining, alongside several bottles of chemicals arranged nearby.
It's been a while since I last grew yeast (Schizosaccharomyces pombe) myself - exciting🤩
A colleague asked - Are you doing experiments? - and I replied -Yes, just a little😉
Also helping me boost my step count, definitely better than sitting in my office all day😊
#Experiment #Science #Pombe #Yeast
Schizosaccharomyces pombe on the cover of JCS - beautiful as usual 😊
My favorite yeast 😉
#Yeast #Pombe
journals.biologists.com/jcs/issue/13...
A postdoctoral position is available in the lab of Henry Levin in the Eunice Kennedy Shriver National Institute of Child Health and Human Development to study the rDNA repeats of Schizosaccharomyces #pombe.
community.alliancegenome.org/t/sequence-v...
#yeast #rDNA #job #postdoc
Our fission yeast are soooo happy 😊 #pombe #meiosis4eva
