iPS2-seq is a clone-aware platform for #singlecell loss-of-function screening in #hiPSCs and derivatives including #organoids, here revealing SMAD2 role in cardiac progenitor specification from @berterolab.bsky.social @mendjanlab.bsky.social #functionalgenomics ➡️ link.springer.com/article/10.1...

A superbly synchronized talk from Joy Zheng & Yan Li, from Mu He's lab, Hong Kong Univeristy on airway epithelial-mesenchymal organoids from #hIPSCs for modelling syndromic #ciliopathies. More mature lineages in a dish. Fascinating work on TZ components and motile cilia function. #UKCilia2025 /2

I'm proud to share that this week I started my position as #MSCA Postdoctoral Fellow in the @paquetlab.bsky.social at #LMUMunich!
For the coming 2 years, I'll optimize a human in vitro vascularized 3D brain tissue model and apply it for the study of #neurodegeneration. 👩‍🔬 🔬 🧠

#hiPSCs
#3DTissueModel

Microglial APOE3 Christchurch protects neurons from Tau pathology in a human iPSC-based model of Alzheimer’s disease Sun et al. demonstrate that APOE3 Christchurch microglia protect neurons from Aβ-induced Tau pathology by regulating lipid peroxidation and phagocytosis. Targeting APOE and lipid metabolism in microgl...

Scientists from @cityofhope.bsky.social , @ucimind.bsky.social , & @udea.bsky.social used neuron-microglia co-cultures & neuroimmune #organoids from #hiPSCs to discover why a certain genetic variant, #APOECh, can delay AD progression. In @cp-cellreports.bsky.social

www.cell.com/cell-reports...

lnkd.in/dcy5vjMv

Nuestro estudio revela cómo la posición de la variante en NOTCH3 influye en la severidad de CADASIL a nivel de células madre pluripotentes inducidas (hiPSCs).

#science #Research #CADASIL #Genetics #StemCells #Neuroscience #TREAT #LINC #Notch3 #hiPSCs

With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄!

This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬

@benjaminbjohnson.bsky.social?

🧑‍🔬💰 #NIMH funding opportunity for #neuroscience researchers working with #hiPSCs, #genomics, #computationalbiology, large human datasets, and more.
grants.nih.gov/grants/guide...

Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes. A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below! https://doi.org/10.1016/j.crmeth.2022.100300 https://doi.org/10.1101/2024.10.17.616637

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social