LPS increases Actb mRNA in PeMPs in vivo and enhances its localized translation in vitro. New synthesis of β-actin in microglial peripheral structures was assessed with a 2-min puromycin pulse in PBS- and LPS-treated cells followed by proximity ligation assay (PLA) with antibodies against puromycin and β-actin. As a negative control, cells were preincubated with the protein synthesis inhibitor anisomycin. Micrographs show the PLA signal in microglia labeled with phalloidin. Arrowheads indicate PeMPs depicted in insets (4 rightmost panels). Scale bars 20 µm (insets 10 µm)

Local #mRNAtranslation in polarized cells is well studied in neurons but what about #microglia? This study shows that #inflammation enhances microglial #RNA localization & translation via RNA-binding protein IMP1/ZBP1, regulating morphology, motility & phagocytosis @plosbiology.org 🧪 plos.io/486ZLMS

LPS increases Actb mRNA in PeMPs in vivo and enhances its localized translation in vitro. New synthesis of β-actin in microglial peripheral structures was assessed with a 2-min puromycin pulse in PBS- and LPS-treated cells followed by proximity ligation assay (PLA) with antibodies against puromycin and β-actin. As a negative control, cells were preincubated with the protein synthesis inhibitor anisomycin. Micrographs show the PLA signal in microglia labeled with phalloidin. Arrowheads indicate PeMPs depicted in insets (4 rightmost panels). Scale bars 20 µm (insets 10 µm)

Local #mRNAtranslation in polarized cells is well studied in neurons but what about #microglia? This study shows that #inflammation enhances microglial #RNA localization & translation via RNA-binding protein IMP1/ZBP1, regulating morphology, motility & phagocytosis @plosbiology.org 🧪 plos.io/486ZLMS

LPS increases Actb mRNA in PeMPs in vivo and enhances its localized translation in vitro. New synthesis of β-actin in microglial peripheral structures was assessed with a 2-min puromycin pulse in PBS- and LPS-treated cells followed by proximity ligation assay (PLA) with antibodies against puromycin and β-actin. As a negative control, cells were preincubated with the protein synthesis inhibitor anisomycin. Micrographs show the PLA signal in microglia labeled with phalloidin. Arrowheads indicate PeMPs depicted in insets (4 rightmost panels). Scale bars 20 µm (insets 10 µm)

Local #mRNAtranslation in polarized cells is well studied in neurons but what about #microglia? This study shows that #inflammation enhances microglial #RNA localization & translation via RNA-binding protein IMP1/ZBP1, regulating morphology, motility & phagocytosis @plosbiology.org 🧪 plos.io/486ZLMS

Multi-omic assessment of mRNA translation dynamics in liver cancer cell lines Scientific Data - Multi-omic assessment of mRNA translation dynamics in liver cancer cell lines

Hey there, #mRNAtranslation buffs! Our latest multi-faceted dataset from liver cancer cell lines is out in #ScientificData: RNA-seq, ribosome run-off/ribo-seq, polysome-seq, pSILAC. rdcu.be/eC86H. Great team effort spearheaded by Asier Gonzalez Sevine and Niels Schlusser. Happy data crunching!

Characterization of ribosome heterogeneity during endothelial to hematopoietic transition

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#Ribosomes #mRNAtranslation

Ribosome association inhibits stress-induced gene mRNA localization to stress granules A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields

Cool new work explaining the dynamics of mRNA translation with the ribosome to inhibit SG localization- by the Moon Lab
#ribosomes #uORF #mRNATranslation #StressResponse
genesdev.cshlp.org/content/earl...

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🧪 Protein synthesis and translational control🧪

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Abstracts Jun 1

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#ProteinSynthesis ##TranslationalControl #mRNAtranslation