🚨new #PhD #opportunity open in our group!
In collaboration with John Tregoning,we are recruiting for a project focused on #engineering #synthetic #vesicle platforms for programmable #immune #training and #vaccine #delivery.
📅 Application deadline: 16th April
www.imperial.ac.uk/medicine/stu...
Top: UNC-11 ∆AD synapses exhibit enlarged synaptic vesicles and abnormal endosome-like structures. Representative transmission electron micrographs of synapses from wild-type N2, unc-11 mutants, Full-length UNC-11-rescued, and UNC-11∆AD-rescued unc-11 mutants. Dense projections are highlighted in orange. The arrow marks an endosome-like structure (ELS, >100 nm in diameter). Scale bar, 100 nm. Bottom: UNC-11 condensates promote the growth of F-actin. Full-length UNC-11 (UNC-11 FL) was labeled with Pacific Blue (cyan), and F-actin was visualized using Alexa Fluor 488–phalloidin (yellow). G-actin (2 µM) and phalloidin (0.2 µM) were added to samples containing UNC-11 droplets, and fluorescence images were collected every 3 min for 48 min. Scale bar, 5 µm.
How does AP180 regulate #SynapticVesicles during #neurotransmission? This study shows that AP180's assembly domain regulates #synaptic #vesicle size & release fidelity in #Celegans via actin binding, revealing how vesicle morphology influences neurotransmission @plosbiology.org 🧪 plos.io/3OcO4g4
Top: UNC-11 ∆AD synapses exhibit enlarged synaptic vesicles and abnormal endosome-like structures. Representative transmission electron micrographs of synapses from wild-type N2, unc-11 mutants, Full-length UNC-11-rescued, and UNC-11∆AD-rescued unc-11 mutants. Dense projections are highlighted in orange. The arrow marks an endosome-like structure (ELS, >100 nm in diameter). Scale bar, 100 nm. Bottom: UNC-11 condensates promote the growth of F-actin. Full-length UNC-11 (UNC-11 FL) was labeled with Pacific Blue (cyan), and F-actin was visualized using Alexa Fluor 488–phalloidin (yellow). G-actin (2 µM) and phalloidin (0.2 µM) were added to samples containing UNC-11 droplets, and fluorescence images were collected every 3 min for 48 min. Scale bar, 5 µm.
How does AP180 regulate #SynapticVesicles during #neurotransmission? This study shows that AP180's assembly domain regulates #synaptic #vesicle size & release fidelity in #Celegans via actin binding, revealing how vesicle morphology influences neurotransmission @plosbiology.org 🧪 plos.io/3OcO4g4
Top: UNC-11 ∆AD synapses exhibit enlarged synaptic vesicles and abnormal endosome-like structures. Representative transmission electron micrographs of synapses from wild-type N2, unc-11 mutants, Full-length UNC-11-rescued, and UNC-11∆AD-rescued unc-11 mutants. Dense projections are highlighted in orange. The arrow marks an endosome-like structure (ELS, >100 nm in diameter). Scale bar, 100 nm. Bottom: UNC-11 condensates promote the growth of F-actin. Full-length UNC-11 (UNC-11 FL) was labeled with Pacific Blue (cyan), and F-actin was visualized using Alexa Fluor 488–phalloidin (yellow). G-actin (2 µM) and phalloidin (0.2 µM) were added to samples containing UNC-11 droplets, and fluorescence images were collected every 3 min for 48 min. Scale bar, 5 µm.
How does AP180 regulate #SynapticVesicles during #neurotransmission? This study shows that AP180's assembly domain regulates #synaptic #vesicle size & release fidelity in #Celegans via actin binding, revealing how vesicle morphology influences neurotransmission @plosbiology.org 🧪 plos.io/3OcO4g4
Research progress and application prospect of engineered small extracellular vesicles
doi.org/10.1177/0963...
#EVs #vesicle #Engineering
@journals.sagepub.com
Location of the selected factors in the different endomembrane system pathways.
Defender or accomplice? Dual roles of plant #vesicle #trafficking in restricting and enabling #geminiviral systemic infection
📖 nph.onlinelibrary.wiley.com/doi/10.1111/...
by Cana-Quijada et al.
@WileyPlantSci #PlantScience
Researchers reported in Horticulture Research that they have combined brefeldin A treatment and antisense oligodeoxynucleotide (AS-ODN) technology to investigate vesicle trafficking in the pollen tubes of Torenia fournieri.
#pollen tubes #vesicle
Details: doi.org/10.1093/hr/uhaf018
#bskyart #artai #iconoplasm #AIArt #AIArtist #AIArtwork #AIimages #GenArt #DigitalArt #AIArtGallery #AIGeneratedArt #AIArtCommunity #synthart #bronze #hands #vesicle #silver #glass #sculpture #sleep #goodnight
Sleep Comes Down [good night]
Engineered extracellular vesicles facilitate delivery of advanced medicines
thanks to @nanoappsmedical.bsky.social @amandascott2023.bsky.social @cellrep7.bsky.social
#NanoTech #NanoApplication #Engineering #extracellular #vesicle #advancedmedicine
www.nanoappsmedical.com/engineered-e...
A) Diagram of the RUSH system. The Halo-tagged cargo is retained in the ER by the binding of the Streptavidin binding peptide (SBP) to Streptavidin (StrepA), which is fused to the ER retention signal, KDEL. The cargo is synchronously released upon the addition of biotin, which outcompetes SBP for binding to Streptavidin.B) Diagram of the tagged cargo constructs used in this study. C) Steady state expression of UAS-SBP-Halo-Cadherin99c (magenta) under the control of traffic jam-Gal4, showing its localization to the apical microvilli. Phalloidin staining of F-actin (green) labels the apical microvilli in the follicle cells and the oocyte. Scale bar 10 µm. D) Steady state expression of UAS-SBP-SNAP-Ndg (magenta) under the control of traffic jam-Gal4, showing its localization to the basement membrane. Actin is shown in green. Scale bar 10 µm. E) En face view of SBP-SNAP-Ndg in the basement membrane. Scale bar 10 µm. F) Time course of SBP-Halo-Cadherin99C trafficking in fixed samples. G) 25 min after release from the ER, Cad99c (green) localizes to subapical puncta that are labeled by Rab11 (magenta). Scale bar 10 µm. In all figures with a cross-section of the follicle cells, apical is toward the top of the image and basal toward the bottom.
How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp
A) Diagram of the RUSH system. The Halo-tagged cargo is retained in the ER by the binding of the Streptavidin binding peptide (SBP) to Streptavidin (StrepA), which is fused to the ER retention signal, KDEL. The cargo is synchronously released upon the addition of biotin, which outcompetes SBP for binding to Streptavidin.B) Diagram of the tagged cargo constructs used in this study. C) Steady state expression of UAS-SBP-Halo-Cadherin99c (magenta) under the control of traffic jam-Gal4, showing its localization to the apical microvilli. Phalloidin staining of F-actin (green) labels the apical microvilli in the follicle cells and the oocyte. Scale bar 10 µm. D) Steady state expression of UAS-SBP-SNAP-Ndg (magenta) under the control of traffic jam-Gal4, showing its localization to the basement membrane. Actin is shown in green. Scale bar 10 µm. E) En face view of SBP-SNAP-Ndg in the basement membrane. Scale bar 10 µm. F) Time course of SBP-Halo-Cadherin99C trafficking in fixed samples. G) 25 min after release from the ER, Cad99c (green) localizes to subapical puncta that are labeled by Rab11 (magenta). Scale bar 10 µm. In all figures with a cross-section of the follicle cells, apical is toward the top of the image and basal toward the bottom.
How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp
A) Diagram of the RUSH system. The Halo-tagged cargo is retained in the ER by the binding of the Streptavidin binding peptide (SBP) to Streptavidin (StrepA), which is fused to the ER retention signal, KDEL. The cargo is synchronously released upon the addition of biotin, which outcompetes SBP for binding to Streptavidin.B) Diagram of the tagged cargo constructs used in this study. C) Steady state expression of UAS-SBP-Halo-Cadherin99c (magenta) under the control of traffic jam-Gal4, showing its localization to the apical microvilli. Phalloidin staining of F-actin (green) labels the apical microvilli in the follicle cells and the oocyte. Scale bar 10 µm. D) Steady state expression of UAS-SBP-SNAP-Ndg (magenta) under the control of traffic jam-Gal4, showing its localization to the basement membrane. Actin is shown in green. Scale bar 10 µm. E) En face view of SBP-SNAP-Ndg in the basement membrane. Scale bar 10 µm. F) Time course of SBP-Halo-Cadherin99C trafficking in fixed samples. G) 25 min after release from the ER, Cad99c (green) localizes to subapical puncta that are labeled by Rab11 (magenta). Scale bar 10 µm. In all figures with a cross-section of the follicle cells, apical is toward the top of the image and basal toward the bottom.
How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp
Luminescent Iridium-Peptide Nucleic Acid Bioconjugate as Photosensitizer for Singlet Oxygen Production toward a Potential Dual Therapeutic Agent | Inorganic Chemistry pubs.acs.org/doi/10.1021/... Cauteruccio, Maggioni, and co-workers @InorgChem #iridium #PNA #1O2 #TPA #vesicle #PDT
Review in this collection: Mechanistic insights into #liver #fluke -induced #bile #duct #cancer, authored by Michael Smout, Thewarach Laha, Sujittra Chaiyadet, Paul Brindley, & Alex Loukas. #Cholangiocarcinoma #CCA #Granulin #Microbiome #Vaccine #Extracellular #Vesicle
www.cell.com/trends/paras...
😎 Interested in investigating how the #MembraneCharge, expansion, and packing influence #MembraneCondensate and #vesicle interactions?
Join the #ExterkateLab in at the @hhu.bsky.social!
Check out our #PhDposition: www.comeincell.org/dc05-biomole...
#ComeInCell #MSCA
New paper out on extracellular #vesicle (EV) mobility in #collagen I #hydrogels!
How #integrins on EV surfaces influence EV movement through tissue-like environments.
pubs.acs.org/doi/10.1021/...
"Cells across the Tree of Life exchange 'text messages' using RNA" [via Quanta Magazine] 🧪
"RNA may be a molecular lingua franca [crossing] taxonomic boundaries [encoding] messages [read by all]"
www.quantamagazine.org/cells-across...
#science #cells #EV #biology #vesicle #messenger #RNA
Mechanisms to separately regulate synaptic #vesicle release and recycling: Interactions of two voltage-gated #calcium channels and a pump enable separate control of #exocytosis and #endocytosis at chemical #synapses www.uni-mainz.de/presse/aktuell/13938_ENG...
Picture of the month July. Congrats! to @AgataWitkowska from @VolkerHaucke lab.
Giant unilamellar #vesicle in the image is roughly 8x8 μm big, generated with live confocal imaging #microscopy. Shown artificially recreated process of cell #membrane deformation. #endocytosis #GUV
