#SpatialTranscriptomics maps glandular #epithelialcells in #ProstateCancer, revealing progression trajectories and pinpointing key genes driving #tumor advancement, with SLC4A4 and H2AFJ validated as promising #biomarkers in advanced disease.

#OpenAccess: doi.org/10.1016/j.ge...

Still two more #ParisPostdocsSeminars before the end of the month!

🧪Dr. Arianna Giannetti 📆 Nov 25th 📍 @parcc-inserm.bsky.social | #Stress #Thrombosis

🧪Dr. Camila Valenzuela-Montenegro 📆Nov 27th 📍 @institutcurie.bsky.social | #Salmonella #EpithelialCells

Don't miss them! #Science #Postdoc

#Medssky🧪 #Cellbiology #Epithelialcells work collectively to provide a protective barrier, yet they turn over rapidly through cell division & death. If the numbers of dividing & dying cells do not match, the barrier can vanish, or tumours can form.

IL-18 signaling in epithelial-derived enterochromaffin cells plays a pivotal role in injury response and stem cell activation in the mouse intestine #gutresearch #epithelialcells #IL18 #stemcells buff.ly/Jafo68c

This week's #ThrowbackThursday in JHC: "Increased ER Stress and Unfolded Protein Response Activation in Epithelial and Inflammatory Cells in Hypersensitivity Pneumonitis."

Click to read for FREE: bit.ly/4ea9C5I

#epithelialcells #inflammation #ERstress

The IL-18 receptor is expressed on murine small-intestinal enterochromaffin cells and executes a recovery program upon injury | PNAS Upon injury, epithelial-derived IL-18 is released and induces an inflammatory response in underlying IL18R1+ lamina propria cells. Notably, Il18r1 ...

IL-18 signaling in epithelial-derived enterochromaffin cells plays a pivotal role in injury response and stem cell activation in the mouse intestine #gutresearch #epithelialcells #IL18 #stemcells
@karapedsgastrodoc.bsky.social
www.pnas.org/doi/10.1073/...

Lipid Nanoparticles for In Vivo Lung Delivery of CRISPR-Cas9 Ribonucleoproteins Allow Gene Editing of Clinical Targets In the past 10 years, CRISPR-Cas9 has revolutionized the gene-editing field due to its modularity, simplicity, and efficacy. It has been applied for the creation of in vivo models, to further understa...

Targeting #Lung #EndothelialCells & #EpithelialCells through #LNPtechnology without off-target editing in the liver offers #DrugDevelopment pubs.acs.org/doi/10.1021/... @MJMitchell_Lab #LipidNanoparticles #Nanomedicine #TranslationalScience

#Covid19 #SARS-CoV-2 #Mouth #EpithelialCells #DNA-Damage

⬇️❗❗❗

A) Diagram of the RUSH system. The Halo-tagged cargo is retained in the ER by the binding of the Streptavidin binding peptide (SBP) to Streptavidin (StrepA), which is fused to the ER retention signal, KDEL. The cargo is synchronously released upon the addition of biotin, which outcompetes SBP for binding to Streptavidin.B) Diagram of the tagged cargo constructs used in this study. C) Steady state expression of UAS-SBP-Halo-Cadherin99c (magenta) under the control of traffic jam-Gal4, showing its localization to the apical microvilli. Phalloidin staining of F-actin (green) labels the apical microvilli in the follicle cells and the oocyte. Scale bar 10 µm. D) Steady state expression of UAS-SBP-SNAP-Ndg (magenta) under the control of traffic jam-Gal4, showing its localization to the basement membrane. Actin is shown in green. Scale bar 10 µm. E) En face view of SBP-SNAP-Ndg in the basement membrane. Scale bar 10 µm. F) Time course of SBP-Halo-Cadherin99C trafficking in fixed samples. G) 25 min after release from the ER, Cad99c (green) localizes to subapical puncta that are labeled by Rab11 (magenta). Scale bar 10 µm. In all figures with a cross-section of the follicle cells, apical is toward the top of the image and basal toward the bottom.

How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp

A) Diagram of the RUSH system. The Halo-tagged cargo is retained in the ER by the binding of the Streptavidin binding peptide (SBP) to Streptavidin (StrepA), which is fused to the ER retention signal, KDEL. The cargo is synchronously released upon the addition of biotin, which outcompetes SBP for binding to Streptavidin.B) Diagram of the tagged cargo constructs used in this study. C) Steady state expression of UAS-SBP-Halo-Cadherin99c (magenta) under the control of traffic jam-Gal4, showing its localization to the apical microvilli. Phalloidin staining of F-actin (green) labels the apical microvilli in the follicle cells and the oocyte. Scale bar 10 µm. D) Steady state expression of UAS-SBP-SNAP-Ndg (magenta) under the control of traffic jam-Gal4, showing its localization to the basement membrane. Actin is shown in green. Scale bar 10 µm. E) En face view of SBP-SNAP-Ndg in the basement membrane. Scale bar 10 µm. F) Time course of SBP-Halo-Cadherin99C trafficking in fixed samples. G) 25 min after release from the ER, Cad99c (green) localizes to subapical puncta that are labeled by Rab11 (magenta). Scale bar 10 µm. In all figures with a cross-section of the follicle cells, apical is toward the top of the image and basal toward the bottom.

How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp

A) Diagram of the RUSH system. The Halo-tagged cargo is retained in the ER by the binding of the Streptavidin binding peptide (SBP) to Streptavidin (StrepA), which is fused to the ER retention signal, KDEL. The cargo is synchronously released upon the addition of biotin, which outcompetes SBP for binding to Streptavidin.B) Diagram of the tagged cargo constructs used in this study. C) Steady state expression of UAS-SBP-Halo-Cadherin99c (magenta) under the control of traffic jam-Gal4, showing its localization to the apical microvilli. Phalloidin staining of F-actin (green) labels the apical microvilli in the follicle cells and the oocyte. Scale bar 10 µm. D) Steady state expression of UAS-SBP-SNAP-Ndg (magenta) under the control of traffic jam-Gal4, showing its localization to the basement membrane. Actin is shown in green. Scale bar 10 µm. E) En face view of SBP-SNAP-Ndg in the basement membrane. Scale bar 10 µm. F) Time course of SBP-Halo-Cadherin99C trafficking in fixed samples. G) 25 min after release from the ER, Cad99c (green) localizes to subapical puncta that are labeled by Rab11 (magenta). Scale bar 10 µm. In all figures with a cross-section of the follicle cells, apical is toward the top of the image and basal toward the bottom.

How are specific cargos targeted to apical & basolateral domains within #EpithelialCells? This study uses a novel #vesicle tracking software "MSP-tracker" to show that the secretory pathway in #Drosophila follicle cells is unexpectedly spatially organized @plosbiology.org 🧪 plos.io/3EfJsBp

There is an #article in #ScienceAlert about how our #epithelialcells #scream when #injured. Like ACTUALLY scream. Yes. My body feels that intensely thanks to #fibromyalgia and #chronicpain.

Flow-Cytometric Quantification of Urine Kidney #EpithelialCells Specifically Reflects #TubularDamage in Acute Kidney Diseases #AKD

#VisualAbstract by @DrPSVali

www.kireports.org/ar...

@penghard.bsky.social @jan.klocke@bsky.social