Glad we could contribute to this beautiful work from Claire Dudley et al! I remain forever in awe of Dr. Daniel Foust’s amazing data visualization skills.

📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work.
Read the paper here: doi.org/10.1021/acsn...

Mirror mirror on the wall who is the brightest of them all?

Brightness is a key parameter for choosing fluorescent reporters in bioimaging. As tricky to assess, we present a pipeline to compare chemigenetic labels and FPs in tissue culture and #zebrafish embryos.

www.biorxiv.org/content/10.6...

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

We've spent the last year building something at @humantechnopole.bsky.social that we think is genuinely needed: #AI that works across biological scales and data modalities, not just within them. A thread on what we're doing, why, and who we're looking for. A 🧵... 1/9

Dissecting the RNA-binding capacity of the multi-RRM protein Rrm4 essential for endosomal mRNA transport Abstract. RNA-binding proteins (RBPs) utilize multiple RNA-binding domains (RBDs) to engage with extensive messenger RNA (mRNA) networks. Understanding the

Breakthrough article 😊
Joint effort of teams Zarnack & König & Feldbrügge clarifies how multiple RNA-binding domains cooperate to identify functional important binding sites in vivo
doi.org/10.1093/nar/...
@zarnack-group.bsky.social
@könig-lab.bsky.social
@mibinet.bsky.social
#EMBOrnaLocalization

What if cells have undiscovered methods for managing their internal logistics? Our recent work (@cathygalbraith.bsky.social, James A. Galbraith, @sciencethecat.bsky.social & myself) challenges existing views on intracellular transportation 🔗 lnkd.in/dhMDgY5Q

Don't miss this one 👇🏻 ... the SMLMS train stops at Stockholm in 2026 and the menu looks great! 🔬

Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort.

www.biorxiv.org/cgi/content/...

Great work, congratulations!
Is there possibly a connection between the 3' binding site of TDP-43 and the 3' loop of NEAT1_2 in PS? 👉🏻 www.nature.com/articles/s41...

Paraspeckle condensation is controlled via TDP-43 polymerization and linked to neuroprotection!

www.nature.com/articles/s41...

Excellent work from Tatyana Shelkovnikova Lab in @natcellbio.nature.com in collaboration @embl.org

www.nature.com/articles/s41...

My first PhD student‘s, Kavan Gor, paper on 5-color single-molecule imaging of co-transcriptional RNA folding is out in @science.org Advances!

He finds how RNA modification enzymes, antisense oligonucleotides and ribosomal proteins re-route nascent RNA folding.

See more details in EMBL post below!

Muller Lab Welcome to the Muller Lab website - here are our latest news:

🚨 Postdoc position open - please repost!
Join our lab in Lausanne🇨🇭to study KSHV, virus–host interactions & RNA decay + great quality of life right by Lake Leman and the Alps ⛰️
Apply here 👉 sites.google.com/view/muller-...

STED microscopy of DNA origami (scale bar = 50 nm) (taken from https://doi.org/10.1021/acsnano.5c21411, Figure S1).

🙏 @crc1507.bsky.social @sfb1177.bsky.social @goetheuni.bsky.social @pubs.acs.org

Molecule counting with STED🔬= fast & 3D & entire cells
👉🏻 we used DNA-fluorophore labels, evaluated intensity-based molecule counting on DNA origami, and measured EGFR dimerization in cells. Great work 💪 from Laurell, Ash and @yunqingli.bsky.social 👉🏻 now out in ACS Nano 👉🏻 doi.org/10.1021/acsn...

Spectral multiplexing is typically limited to 4-5 channels. Our new preprint introduces a framework that utilizes DNA barcoding and signal tuning to enable robust spectral unmixing and ground-truth benchmarking to achieve 15-plex subcellular profiling without cycling
www.biorxiv.org/content/10.6...

DNA-PAINT with photocaged DNA labels = PhotoPAINT🔬. Why? No need for washing or mixing 👉🏻 higher accuracy in DNA-PAINT & other super-res methods.
Fantastic collab with Alex Heckel & driven by the 'magic team' Nina Kaltenschnee and Marina Dietz 💪
doi.org/10.1002/anie...
@goetheuni.bsky.social

We are excited to share our new preprint: iCLIP3 — an improved protocol for transcriptome-wide mapping of protein–RNA interactions at single-nucleotide resolution.

New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

We are excited to be recruiting a new tenure track group leader in the Structural Studies Division at MRC LMB! It is an amazing place to start your own lab.
@mrclmb.bsky.social

Please get in touch if you have any questions.
www.nature.com/naturecareer...

The gray planet symbolizes a cell that appears lifeless at first glance. Lectins, depicted as a UFO, illuminate and reveal its glycans, transforming it into a vibrant molecular landscape and highlighting the hidden complexity and biological significance of cellular nanoarchitecture.

Our Glyco-STORM paper is officially out in the 23 Feb 2026 issue of #AdvancedScience: doi.org/10.1002/advs....
Celebrating with the cover artwork🎉.
What looks gray and lifeless at first glance unfolds into complex molecular landscapes when revealed with lectin probes & super-resolution microscopy.

We’re still at the @biophysicalsoc.bsky.social
#bps2026 Meeting in San Francisco! Come visit us at Booth 630 and take a #cellfie with us! 📸! Interested in a fully funded PhD through IMPRS-CBP? This is your chance to meet us, ask questions, and learn more about #PhDpositions in Germany. #biophysics

⚠️ 1 week left for Pasteur Course "Advanced Bioimage Analysis With AI"! 👩‍🔬💻

Intensive course (June 1-5, Paris): theory + hands-on, AI focus in bioimaging.

📍 Institut Pasteur
🗣️ English
⏰ Deadline: March 1

🔬 Beginner basics, batch scripts, interactive sessions

👉 Apply: https://bit.ly/4c8rFKZ

Xiaofeng combined 2D live-cell single-particle tracking with 3D simulations to specifically measure the bacterial nucleoid accessibility and viscosity! Our preprint describes how these nucleoid properties respond to cellular processes! Code is available too.
www.biorxiv.org/content/10.6...

Thanks for highlighting our work on combining AI-based organelle dynamics detection in phase contrast with fluorescence!

www.nature.com/ncomms/edito...

@natcomms.nature.com and Nelio Rodriguez. Spearheaded by @wl-stepp.bsky.social with collaborators @jclandoni.bsky.social @maweigert.bsky.social

Our new paper's out: FidlTrack—structure-aware single-particle tracking benchmarks/boost SPT fidelity

With it we resolve with sub-organelle res. e.g. BACE1 amyloidogenic APP cleavage #Alzheimers, ER exit events, map nanobody binding in realtime in ER/organelles 🔬🧠#SingleMolecule

rdcu.be/e3Ris

PhD in Super-Resolution microscopy of clinical biopsies for precision medicine

We are hiring! We’re looking for a PhD candidate to apply super-resolution microscopy to clinical biopsies, bridging advanced imaging and precision medicine. Perfect for researchers passionate about microscopy and eager to make an impact in healthcare. Apply here: www.tue.nl/werken-bij-t...

We switch between targets using light ... more soon!

🔬we have been playing with 'an old tool from (DNA) chemistry' 🧪 and used that for DNA-based confocal/super-res microscopy ... these are the last recordings for the revision and were recorded last night ... 🙏 thanks to R1 for this suggestion: