Smart Secondaries® - NanoTag Biotechnologies Ideal secondary Ab replacement for One Step IF, higher resolution & species-independent multiplexing. Discover benefits of Smart Secondaries®!

Never worked with #SmartSecondaries® (secondary nanobodies) for detecting conventional or recombinant primary antibodies? Here you can find more information: nano-tag.com/technology/s...

📸 Left panel: PFA-fixed rat brain stained with rHcAb anti-CNPase Rb IgG Fc-fusion, detected with Smart Secondary® – FluoTag®-X2 anti-Rb IgG.
Right panel: PFA-fixed rat primary neurons stained with rHcAb anti-SYT1 (7D5) Ms IgG1 Fc-fusion, detected with Smart Secondary® – FluoTag®-X2 anti-Ms IgG1.

📣 Neuroscientists – something NEW for you!

NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬.

Learn more 👉 bit.ly/4cKITOd

#Neuroscience #RecombinantAntibody

📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work.
Read the paper here: doi.org/10.1021/acsn...

🌸 Springtime – where flowers and ideas bloom! Whether you’re chasing big questions or exploring tiny molecules, our #nanobody tools & custom #sdAb services help you make egg-cellent progress. 🐣🔬🦙

Wishing you flourishing research and plenty of hoppy moments 🐇!

#HappyEaster from #NanoTag!

Meet #NanoTag at the #FOM2026 in Stockholm!
Visit booth 24 to explore our 🦙 #nanobody -based solutions tailored for quantitative super-resolution microscopy and flexible #multiplexing 🔬.

Our team is looking forward to connecting with you!

#SingleDomainAntibodies
#SmartSecondaries #FluoTag

Join #NanoTag team at #FOM2026 in Stockholm 🇸🇪!

Visit booth #24 to see how our single-domain antibody (sdAb) technologies bring greater precision to #microscopy and more flexibility & ease to #multiplexing 🔬

See you in Stockholm! 👋

#SuperResolution #Nanoscopy
#Nanobody #Immunofluorescence

📄🦠 New publication! In collaboration with #Takeda our synthetic single-domain antibody library enabled rapid discovery of strain-specific sdAbs against SARS-CoV-2 variants, supporting vaccine identification testing.
Read more in Antibodies @mdpiopenaccess.bsky.social
👉 doi.org/10.3390/anti...

For flexible HaloTag-based imaging, explore our Halo fusion nanobody portfolio or contact us for custom solutions 👉 bit.ly/3OEXiSK

📄🔬 Congratulations to all authors on this excellent preprint showcasing #NanoFLex, a FLIM strategy that enables lifetime-based separation of multiple targets in a OneStep-IF using our #SmartSecondaries fused to different #HaloTag variants. 👏

We’re proud that SmartSecondaries® power this workflow☺️

We’re having a fantastic time at #BPS2026 in San Francisco 🌁

Last chance to stop by booth 720 to meet the #NanoTag team and learn how our nanobody-based reagents 🔬 and sdAb services 🦙 can advance your research.

Thanks for the great discussions and the positive feedback on our #sdAb technologies💙!

Special thanks to 𝐒𝐚𝐛𝐫𝐢𝐧𝐚 𝐏𝐮𝐭𝐢𝐠𝐧𝐚𝐧𝐨 (FU Berlin @freieuniversitaet.bsky.social) for sharing these D. melanogaster multiplexing images with us 💙

Did you know #multiplex IF can be done in just one incubation step?

❌ No sequential staining
❌ No host-species limits
❌ No secondary-induced clustering

🔬Pre-mix your favourite primary antibodies (even same-species) with Smart Secondaries® #nanobody & apply directly to your sample for OneStep IF.🤩

Curious about FluoTag®-X2 anti-PSD95? 🔬
Read the characterization paper here:
www.mdpi.com/1422-0067/24...
Explore product details here:
nano-tag.com/product/fluo...

Neuroscientists - looking for a high-affinity gift?💝

Inside the postsynapse, proteins crowd the show
PSD95’s the star - but hard to know.

IgG antibodies knock, but can’t get through
Too bulky to reach the target they pursue.

But FluoTag®-X2 anti-PSD95 slips right in
Binds PSD95. Precise within.

Heading to #BPS2026 in San Francisco?

Visit NanoTag team at booth 720 to explore our single-domain antibody #sdAb tools: ALFA® System, FluoTags®, Smart Secondaries® & Selector Resins.
See you! 👋

@biophysicalsoc.bsky.social
#Nanobody #Multiplexing
#SuperResolution #Microscopy
#AffinityPurification

📄This new Journal of Microscopy 🔬 paper uses rigorous single-molecule assays to benchmark secondary nanobodies and shows NanoTag #SmartSecondaries® deliver high-affinity binding, efficient saturation, and exceptionally low off-target labeling for robust #multiplexed immunoassays 💪

💬 Thank you, Bhanu Prakash Potlapalli, for the great feedback on the ALFA® system! 😊 Applied in a CRISPR–dCas9 DNA imaging approach for efficient labeling of repetitive DNA sequences and live-cell imaging of plant telomeres doi.org/10.1093/jxb/...

Explore #ALFAsystem 👉 nano-tag.com/technology/t...

Did you know? #NanoTag offers custom single-domain antibody (sdAb) discovery services, including alpaca immunization🦙and synthetic library–based approaches🧬backed by over a decade of experience in the field 💪.

Discuss your project with our experts: nano-tag.com/contact/

#CRO #Nanobody #VHH #sdAb

🔬 Join Imaging Bites seminar 28 Jan at 12:30 at @kingscollegelondon.bsky.social.
Prof. Dr. Felipe Opazo @opazo.bsky.social presents #OneStep-IF, a simplified approach to highly #multiplexed IF using same-species primaries and Smart Secondaries® premixes.

Register here: www.kcl.ac.uk/events/imagi...

✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners.
Here’s to what’s next 🥂

#CellBio2025 in Philadelphia has come to a close!
Thanks to everyone who stopped by the #NanoTag booth 🦙 – great conversations and insights all around.
We were excited to hear how our sdAb tools are driving your new discoveries 📄👏

#SingleDomainAntibody
#ALFAsystem
#SmartSecondaries

What most people don’t see is the behind-the-scenes effort- not magic, just #teamwork. Karina & Steffen have #NanoTag booth ready for #CellBio2025 in Philadelphia. Starting Sunday, discover how our single-domain antibody solutions can support your research🔬🦙.
See you ascbiology.bsky.social 👋

Attending #CellBio2025 in Philadelphia Dec 6–10?
Visit #NanoTag team at booth #1117 to explore our #sdAb technologies & ready-to-use reagents: ALFA® System, FluoTags®, Smart Secondaries®, Selector Resins.
See you there! 👋 @ascbiology.bsky.social

#Nanobody #CellBiology
#Immunofluorescence #PullDown

Join our CTO, Felipe Opazo @opazo.bsky.social, at #MBSJ2025 @mbsj-official.bsky.social as he presents new super-resolution #multiplexing advances powered by #SmartSecondaries®.
🔬Discover flexible, scalable and precise multi-target imaging.

#Nanobody
#NanoPlex #SUMPAINT
#SuperResolution #Microscopy

Thanks, Nicolas💙! Clean, simple, and keeping the focus where it belongs #Science

🌱🔬 #FluorescenceFriday Beautiful plant microscopy revealed with the ALFA-tag & NbALFA. A recent study by @plantubiquitin.bsky.social et al in Plant Commun shows how well #ALFA®system works in planta. From imaging #SuperResolution to protein interactions, degradation and more.
doi.org/10.1016/j.xp...

Interested in the ALFA® System — nanobodies (NbALFA) recognizing a rationally designed 13aa ALFA-tag — for your POI labeling, imaging, purification and more? Browse our ready-to-use reagents here 👉 bit.ly/47CyZud or contact us to discuss custom solutions.

ALFA nanobody-guided endogenous labeling - Nature Chemical Biology Small peptide tags (like ALFA) cause minimal disruption of proteins, but they typically lack fluorescence, which would enable knockin screening. Now, antigen-stabilizing fluorescent protein-fused nano...

Have you heard of #ANGEL?
Not this 😇, but ALFA Nanobody-Guided Endogenous Labeling #ANGEL - the new approach published by Wang et al. in @natchembio.nature.com Build on our ALFA® System, it enables direct fluorescent readout of #CRISPR knock-ins.

📄Full paper here: bit.ly/47XgK48