I told you it was final 😉, now we celebrate 🎉

This is why I’m not allowed in the wet lab anymore 🥼 🕺. My technique is too effective! 🤪

#PILife #TeamTomo 🧪

Now THIS one was quite the journey. I'm beyond happy to see this work out in the world 🥹

Huge congrats to @computingcaitie.bsky.social and also to @hugovdhoek.bsky.social and everyone else involved ♥️

with @centriolelab.bsky.social @stearnslab.bsky.social @cellarchlab.com

#Cilia #CryoET #TeamTomo

It’s done!! Molecular architecture of the ciliary base in mammalian multiciliated cells! A study 13 years in the making 👴, thanks to collab with @stearnslab.bsky.social + @centriolelab.bsky.social & visionary work by @computingcaitie.bsky.social, who integrated native #cryoET with #XLMS & #UExM 🧪🧶🧬🔬

Crazy things have been happening in cilia world, catch up on them in Caitie's new paper! 🪱

#cilia #cryoet #proteomics

And over the years several people in the @cellarchlab.com contributed to this work including @phaips.vd.st and @lifeonthewedge.bsky.social

This project was started over 10 yrs ago between @cellarchlab.com and @stearnslab.bsky.social, I think I was taking organic chemistry at the time 😝. @hugovdhoek.bsky.social heroically put in a huge effort to get these cells on grids and acquire some beautiful tilt series 💪

Using this powerful combo of tools we observe other features of the ciliary base including the ciliary necklace aligned w the transition zone, IFT trains present at mature cilia and undocked centrioles near ciliary vesicles, and a cytoskeleton network of bundled actin and intermediate filaments

While XL/MS provided several candidates for microtubule binding proteins, the subciliary localization was further verified by @marinebrunet.bsky.social w U-ExM. This combo identified several new microtubule binding proteins including DNAJB6, which is abundant in the transition zone and axoneme

To supplement our cryo-ET and generate candidates for proteins that localize to different ciliary regions, we took a trip to the local butcher to pick up some cow trachea to build a mammalian multiciliated cell interactome using XL/MS (enjoy this vid of @cellarchlab.com shaking cilia off trachea)

Using subtomogram averaging and classification, we map out structural changes along the microtubules of the ciliary base, highlighting densities specific to each region. The transition zone is marked by distinct features including the A-B linker, outer helical wrap, MIP helix, and absence of NME7

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

I’m an optimist 👀

What if you could cut out sub cellular structures and measure the proteome? SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection
www.biorxiv.org/content/10.6...
awesome work by Curdin Franziscus, @imcf-lex.bsky.social,
@biehli.bsky.social, Alexander Schmidt!

New protein complex stoichiometry prediction tool from talent PhD student @daniil-litvinov.bsky.social, check out the thread below!

Exciting opportunity to work in a lab that’s pushing the boundaries of environmental sampling and work with an amazing scientist!!! 👩‍🔬🔬

Final version @nature.com of our paper describing unconventional multicellular development in a choanoflagellate inhabiting an extreme environment. A ton of new data since the first @biorxivpreprint.bsky.social preprint (which we've kept updating).

A brief 🧵 (carried over from the old place)

Nice collab w my neighbor in Basel @abimoye.bsky.social on SAXO6 mol bio and genetics!!

Thrilled to share that our paper on a novel IRD gene has been published in The American Journal of Human Genetics (AJHG)! 🎉 This wouldn’t have been possible without the incredible group of colleagues and collaborators. Special thanks to @computingcaitie.bsky.social, Ditta Zobor, and ERDC members!

📏🔬In collaboration with @computingcaitie.bsky.social and @cellarchlab.com, SAXO6 localization was mapped along ciliary microtubules with direct links to α-tubulin, linking this structural protein class to human retinal disease for the first time.
👉 @ajhgnews.bsky.social
#Ophthalmology

Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵

Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!

Congrats Helen!! Really cool story

For #ECR’s:

DBG’s Hot Tpoic Workshop
on Cryo-Electron Tomography + FIB-milling

13 – 15 April 2026
In @cellarchlab.com 's lab @biozentrum.unibas.ch

Organised by @tamb-o.bsky.social, Karen Zinzius, @fannyleblanc.bsky.social

Details: www.deutsche-botanische-gesellschaft.de/en/about-us/...

You like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint!

What a great collaboration this has been!

A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function - Nature Communications Here they combine in vivo imaging with proteomics to characterize a protein complex comprised of Ccdc78 and Ccdc33 that acts at the extreme distal tip of 9 + 2 motile cilia to control tip architecture...

Cilia alert! Stoked to have the new paper from Juyeon Hong about a new domain at the extreme distal tip of motile cilia! (The EDT, y'all!) It's out now @natcomms.nature.com.
Check it out!
#cilia

www.nature.com/articles/s41...

🔬🚨New preprint alert! 🚨🔬

We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology

www.biorxiv.org/content/10.6...

#SuperResolution #CellBiology

Our research scientist Núria Ros-Rocher won the 1st prize @zeiss-microscopy.bsky.social microscopy contest with this beautiful choano colony www.zeiss.fr/microscopie/... 🥳

C. flexa might not yet be a genetic model, but it is now at least a calendar model, which counts for something. I think.

This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell!

This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍

[Maybe long thread ahead]