Luminal cells reorganize their microtubule cytoskeleton during branching morphogenesis. (A) Mammary organoid culture workflow. (B) DIC timelapse imaging of a representative organoid undergoing branching morphogenesis, following addition of FGF2. (C,D) Super-resolution images of mammary cysts (No GF) and buds (+FGF2). Insets show examples of luminal cells in simple cuboidal epithelium (orange) and stratified epithelial buds (cyan, inner; magenta, outer). (C) High magnification live imaging of microtubule organization of an elongating bud from a branching organoid (+FGF2) and a region of polarized epithelium from a control (NoGF) organoid, using vital dye (white=50 nM SiR-Tubulin, red=mTomato). (D) Quantification comparing fluorescence intensity of SirTubulin and mTomato (mT) between inner stratified and outer stratified cells of elongating buds (n=6 TEB regions from two biological replicates). ns, not significant; ***P<0.0001.
Andrew Fraser & co from @jhu.edu reveal that #microtubules are essential for the collective migration of luminal cells and for mammary branching morphogenesis. doi.org/10.1242/bio....
#BranchingMorphogenesis #MammaryEpithelium
