In clever expts by Inflammasome Lab's Sabrina Burgener, DCs reacted to the calcium-sensitive filopodia of corpses by phosphorylating SYK via #CLEC9A. While #NINJ1-driven #CellExplosion was not needed for filopodial assembly (L; M. Monteleone), it was required to expose F-actin to #CLEC9A🤯. (11/13)

Why do #pyroptotic cells use their final minutes to make #filopodia? Well, #filopodia are rich stores of F-actin under the plasma membrane. Could #CellExplosion during #pyroptosis reveal filopodial F-actin to engage the corpse antigen sampling DC receptor, #CLEC9A/#DNGR1? (10/13) (credit: BioRender)

Incredibly, minutes before pyroptotic #CellExplosion, cells assemble Myo10-capped #filopodia, which crown the resultant cell corpse. #inflammasome #GSDMD signalling was required for both filopodial assembly & cell death. Might Ca2+ influx via GSDMD pores be involved? 🤔(4/13)