RE-read this important paper on small GTPases (Rac/Rho) in Smooth Muscle Cell responses to Matrix Stiffness

Distinct Roles of YAP & TAZ in Mechanotransduction (to SMC priliferation)😎

PAGE gel matrix 0.1-50 kPa

Richard Assoian lab #CellRep 2021
www.cell.com/cell-reports...

Amazing #2PM real-time morpho-dynamic responses of microglia to laser-induced parenchymal/vascular injury in🐭🧠

Age-dependent reaction speed
👉Parenchymal injury
Adult > Aged/Neonatal
👉Vascular injury
Adult/Aged > Neonatal

@vcoelhosantoslab.bsky.social #CellRep 2025
www.cell.com/cell-reports...

Myocilin marks the glia limitans superficialis Astrocyte

Re-analysis of brain-wide #SpatialTranscriptomics & sc-/sn-RNAseq data➡️
Myoc+ 2% of total astrocytes covering brain/spinal cord surface

@philiphasel.bsky.social
@liddelowsa.bsky.social
#CellRep 2025
www.cell.com/cell-reports...

SETD2 H3K36 methyltransferase
Pancreas development
e11.5/13.5/15.5

PDX+ pancreatic progenitor Setd2 KO🐭
👉⏫Exocrine cell proliferation + perturbed tip-to-acinar trajectory
👉⏬α/β Islets ⏫blood glucose

NKX2.2➡️endocrine specification

#CellRep 2024
www.sciencedirect.com/science/arti...

An integrated proteomics approach identifies phosphorylation sites on viral and host proteins that regulate West Nile virus infection Walter et al. used proteomics to investigate changes to protein abundance and post-translational modification during West Nile virus infection. Upregulated proteins like HERPUD1 function to restrict v...

An integrated proteomics approach identifies phosphorylation sites on viral and host proteins that regulate West Nile virus infection #CellRep #MassSpec www.cell.com/cell-reports...

High-confidence glycosomal membrane protein inventory unveils trypanosomal peroxin PEX15 Trypanosoma and Leishmania infections cause devastating neglected tropical diseases. These parasites harbor peroxisome-related organelles called glycosomes, which are essential for their survival. Kri...

High-confidence glycosomal membrane protein inventory unveils trypanosomal peroxin PEX15 #CellRep #MassSpec www.cell.com/cell-reports...

#BloodBrainBarrier

Induced #EndothelialCell Claudin 5 KO (but not Cldn5 -/+) in adult🐭➡️ #BBB leakage (1~10 kDa, but not 70 kD)

Cldn5-null ECs (15% of total ECs) have minimal vs Cldn5-intact ECs➡️non-cell-autonomous impact secondary to BBB leak

#CellRep 2024
www.sciencedirect.com/science/arti...

#YAP #Mechanoptosis #RigiditySensing

pTyr357 YAP, phospho by c-Abl/Src in #FocalAdhesion then translocated to Nuclei, ➡️Apoptosis of MDA-Tpm2.1; MEF on soft matrix 0.25kPa

Cellular sensing of #MatrixStiffness dictates proliferation/apoptosis switch of YAP

#CellRep 2024
www.cell.com/cell-reports...

#PoloLikeKinase1 overexpression🐭->
#ChromosomalInstability #Aneuploidy #Ployploidy #Senescence in #BreastCancer
⏫SASP (IL1RN/IL6/CXCL1)
⏫PD-L1
Reshaped immune niche:
⏫Treg⏫Dendritic cell⏫Arg1+TAM⏬NK

PLK1-hi👤breast tumors->⏫Immunopressive features

#CellRep 2023
www.sciencedirect.com/science/arti...