Chitin organization during corneal lens morphogenesis. Top row: apical surfaces of wild-type (white1118) mid-pupal retinas stained for chitin with chitin-binding domain probe CBD (green) and E-Cadherin and N-Cadherin (magenta). From left to right, the times are 48 h after puparium formation (APF), 50 h APF, 51 h APF, 52 h APF, and 54 h APF. Middle row shows enlargements of single ommatidia. Bottom: Diagram of chitin (green) organization during corneal lens development. CC, cone cells; 1º, primary pigment cells; 2º, secondary pigment cells; 3º, tertiary pigment cells; B, mechanosensory bristles.
How does the #corneal #lens in the #fly eye get its light-focusing shape? @nehaghosh.bsky.social &co show that central cells produce large amounts of #chitin to form the thick central corneal lens; peripheral cells produce smaller amounts to form tapered lens edges @plosbiology.org 🧪 plos.io/4lLNgeY