#Review
Epigenetic drugs in cancer therapy: mechanisms, immune modulation, and therapeutic applications doi.org/10.1186/s435...
#EpigeneticRegulation #DNAmethylation #HistoneModifications #TumorImmunity #ClinicalTranslation
Lactoferrin supplementation modulates the oxidative and metabolic genes by NR5A2-mediated histone modifications in deoxynivalenol-induced ileum injury
Xudong Guo, Xiaoyue Yuan ... Dejun Ji, Yuting Guo
link.springer.com/article/10.1...
#Histonemodifications #ROS #DON
#Medsky🧪 ##immunosky #publichealth An overview of the roles of 2 key epigenetic mechanisms, #DNAmethylation & #histonemodifications, in the toxicity of the most significant environmental pollutants (air pollution , heavy metals
www.cell.com/iscience/ful... @cp-iscience.bsky.social
Top: In Cytometry by Time of Flight (CyTOF), cells are stained with metal-conjugated antibodies, here mainly targeting modified histones, and epitope abundance is then quantified in multiplex in large numbers of individual cells. The data can be analyzed mark by mark, as well as across all the marks using dimensionality reduction approaches, to reveal subpopulations and assess heterogeneity. Bottom: Griess and colleagues explore the consequences of KMT2D null and EZH2 gain-of-function mutations on histone modification profiles in lymphoma cells using CyTOF. They discover that EZH2 alterations are epistatic to KMT2D loss of function and increase both the epigenomic and transcriptomic heterogeneity of the cells. They identify “extreme” and “WT-like” subpopulations of EZH2-mutant cells that form dynamically and show distinct responses to small-molecule inhibitors.
Raphael Margueron & @danielholoch.bsky.social explore a @plosbiology.org study that uses single-cell measurement of #HistoneModifications to reveal that EZH2 GoF mutations reprogram chromatin states in B-cell #lymphoma 🧪Paper: plos.io/4mZcS8n Primer: plos.io/4l9WArE
Top: In Cytometry by Time of Flight (CyTOF), cells are stained with metal-conjugated antibodies, here mainly targeting modified histones, and epitope abundance is then quantified in multiplex in large numbers of individual cells. The data can be analyzed mark by mark, as well as across all the marks using dimensionality reduction approaches, to reveal subpopulations and assess heterogeneity. Bottom: Griess and colleagues explore the consequences of KMT2D null and EZH2 gain-of-function mutations on histone modification profiles in lymphoma cells using CyTOF. They discover that EZH2 alterations are epistatic to KMT2D loss of function and increase both the epigenomic and transcriptomic heterogeneity of the cells. They identify “extreme” and “WT-like” subpopulations of EZH2-mutant cells that form dynamically and show distinct responses to small-molecule inhibitors.
Raphael Margueron & @danielholoch.bsky.social explore a @plosbiology.org study that uses single-cell measurement of #HistoneModifications to reveal that EZH2 GoF mutations reprogram chromatin states in B-cell #lymphoma 🧪Paper: plos.io/4mZcS8n Primer: plos.io/4l9WArE
Top: In Cytometry by Time of Flight (CyTOF), cells are stained with metal-conjugated antibodies, here mainly targeting modified histones, and epitope abundance is then quantified in multiplex in large numbers of individual cells. The data can be analyzed mark by mark, as well as across all the marks using dimensionality reduction approaches, to reveal subpopulations and assess heterogeneity. Bottom: Griess and colleagues explore the consequences of KMT2D null and EZH2 gain-of-function mutations on histone modification profiles in lymphoma cells using CyTOF. They discover that EZH2 alterations are epistatic to KMT2D loss of function and increase both the epigenomic and transcriptomic heterogeneity of the cells. They identify “extreme” and “WT-like” subpopulations of EZH2-mutant cells that form dynamically and show distinct responses to small-molecule inhibitors.
.@danielholoch.bsky.social & Raphael Margueron explore a @plosbiology.org study that uses single-cell measurement of #HistoneModifications to reveal that EZH2 GoF mutations reprogram chromatin states in B-cell #lymphoma 🧪Paper: plos.io/4mZcS8n Primer: plos.io/4l9WArE
Profiling histone marks with TaDa. Top left: Schematic showing methods used for Targeted DamID profiling of histone modifications. Bottom left: Illustration demonstrating fusion of Dam to chromatin-binding domains for H3K4me3 or H3K27me3 and to mintbodies (scFvs) against H3K9ac or H4K20me1. Right: Histone marks near the Mira locus (shaded) in Drosophila third-instar neural stem cells.
Current methods for profiling #HistoneModifications require cell isolation & don't work with all tissue types. @jellevda.bsky.social &co adapt Targeted DamID (TaDa) to profile cell-type-specific #histone marks throughout the genome, in vivo, without cell isolation 🧪 @plosbiology.org plos.io/3Dw8sUD
Profiling histone marks with TaDa. Top left: Schematic showing methods used for Targeted DamID profiling of histone modifications. Bottom left: Illustration demonstrating fusion of Dam to chromatin-binding domains for H3K4me3 or H3K27me3 and to mintbodies (scFvs) against H3K9ac or H4K20me1. Right: Histone marks near the Mira locus (shaded) in Drosophila third-instar neural stem cells.
Current methods for profiling #HistoneModifications require cell isolation & don't work with all tissue types. @jellevda.bsky.social &co adapt Targeted DamID (TaDa) to profile cell-type-specific #histone marks throughout the genome, in vivo, without cell isolation 🧪 @plosbiology.org plos.io/3Dw8sUD
Profiling histone marks with TaDa. Top left: Schematic showing methods used for Targeted DamID profiling of histone modifications. Bottom left: Illustration demonstrating fusion of Dam to chromatin-binding domains for H3K4me3 or H3K27me3 and to mintbodies (scFvs) against H3K9ac or H4K20me1. Right: Histone marks near the Mira locus (shaded) in Drosophila third-instar neural stem cells.
Current methods for profiling #HistoneModifications require cell isolation & don't work with all tissue types. @jellevda.bsky.social &co adapt Targeted DamID (TaDa) to profile cell-type-specific #histone marks throughout the genome, in vivo, without cell isolation 🧪 @plosbiology.org plos.io/3Dw8sUD
Profiling histone marks with TaDa. Top left: Schematic showing methods used for Targeted DamID profiling of histone modifications. Bottom left: Illustration demonstrating fusion of Dam to chromatin-binding domains for H3K4me3 or H3K27me3 and to mintbodies (scFvs) against H3K9ac or H4K20me1. Right: Histone marks near the Mira locus (shaded) in Drosophila third-instar neural stem cells.
Current methods for profiling #HistoneModifications require cell isolation & don't work with all tissue types. @jellevda.bsky.social &co adapt Targeted DamID (TaDa) to profile cell-type-specific #histone marks throughout the genome, in vivo, without cell isolation 🧪 @plosbiology.org plos.io/3Dw8sUD
Epigenetics Update - Histone mark age of human tissues and cell types bit.ly/40hFAba
From Lucas Paulo de Lima Camillo and Ritambhara Singh, reporting in Science Advances
#Epigenetics #Aging #HistoneModifications
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High-resolution insights with no cell sorting; find out more at epigenome.us
Epigenetics News - Histone modification important for correct blood cell formation bit.ly/4iO5uua
Research by LMU molecular biologist Gunnar Schotta
#Epigenetics #News #HistoneModifications
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Empower your research with high-res epigenetic insights at epigenome.us
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Special issue: Novel insights and future directions in avian ecological epigenetics
More info: www.avianbiology.org...
Editors: @BerniceSepers @rebeccas_chen @KvanOers @CarolineIsak
#ornithology #epigenetics #DNAmethylation #histonemodifications #microRNA #birds
