@Aruthak.bsky.social Yes! The #JEDIs are Innovating & JOE YEONG is pulling us together & time not issue 4 this dedicated group still on a hi from #multiomics2024 /10pm #UK, 6am #Singapore & #Taipei, 8am #AU , 2pm #OR #SpatialMedicine #SpatialCatchUps #SpatialInnovation #FinishCancer #cancer
#Multiomics2024 was amazing and I was very proud to be invited to speak for @standardbiology.bsky.social. Here’s the cartoon version.
Huge thanks to @aruthak.bsky.social for bringing it all together. #spatialbio
What a week at #Multiomics2024 in Brisbane! 🙌 Thanks to everyone who visited our booth to explore the #AVITI24 system with the Element and TrendBio teams. Want to learn more? Join us at the #ShiftYourScience roadshow!
📍Melbourne 🔗 bit.ly/41kyy6q
📍Sydney 🔗 bit.ly/4iot54L
If you didn't join #multiomics2024, here is a nice illustrative summary of my talk on normalisation in spatial txomics data.
TL;DR - Use SpaNorm, the only spatially aware normalisation method out there! We are improving as we learn more so stay tuned for updates!
Preprint doi.org/10.1101/2024...
The extracellular matrix is a collection of proteins changing over time and space. It has different profiles for different cancer subtypes & profiles.
-- Amelia Parker #multiomics2024 /5
Typically 3D imaging is done for proteins. A new pipeline that allows 3D imaging of RNA spatial distributions:
www.biorxiv.org/content/10.1... from Zoe West #multiomics2024 3/
Single cell profiling obscures profound spatial heterogeneity.
Even if these 4 samples had the same proportions of cell types, the spatial arrangement is vastly different.
@lochlanfennell.bsky.social #multiomics2024 1/
Incorporating spatial information improves annotation accuracy & robustness.
-- Yuqi Tan pubmed.ncbi.nlm.nih.gov/36280720/ #multiomics2024 8/
Note: many of the #multiomics2024 speakers are also sharing neat unpublished/not-yet-public work (which I am not posting) 6/
Library size confounds biology in spatial transcriptomics data.
Single cell RNA-seq tools & ideologies will NOT translate to spatial molecular data!
genomebiology.biomedcentral.com/articles/10....
@bhuvad.bsky.social #multiomics2024 4/
Dharmesh maybe not loving the spotlight (in his eyes) 🔦🔦🔦🤣🤣🤣 #SpaNorm #multiomics2024 @bhuvad.bsky.social
Spatial biomarkers are associated with immunotherapy response & can help treatment decision making
Tumor cell subtyping of non-small cell lung cancer found 5 distinct tumor clusters which differed in outcomes
-- Ettai Markovits of NucleAI kicking off computational bio session #multiomics2024 1/
We’re having a great time at #Multiomics2024 in Brisbane. Our Associate Director of Product Management Abbey Cutchin presented about #AVITI24— a single platform for both next gen sequencing and spatial #multiomics. Visit our booth and learn how we’re expanding what’s possible on a benchtop platform.
Watching @anandpatellab.bsky.social give a masterclass in collaboration for #multiomic analysis of #neuroblastoma #multiomics2024
One of the Multi-Omics O.G.s, @alexswarbrick.bsky.social from @garvaninstitute.bsky.social, speaking on charting an atlas of #cancer #ecosystems with single-cell and spatial omics #multiomics2024
Finished talk with an appeal to join @bsky.app
What an incredible evening at the #MultiOmics2024 Gala Dinner! What I heard aptly described as "the largest convening of kings behind spatial biology and multi-omics—right here in Brisbane!"
Our video was well-recieved #philanthropyinaction
Similar to @antobeck.bsky.social, I'm gutted to be missing the second installment of @aruthak.bsky.social epic #multiomics2024 meeting in Brisbane!
Do check out the #Illumina booth and say hello to Ivonne Petermann, Tamsin Eades and Sara Gabrielli
Fighting cancer in 3D with #multiomics2024
Since I cannot attend #multiomics2024 for Illumina, I am going to drop this little hint here on BlueSky for the viewers. Interested in learning more? See Ivonne at the booth and ask her loads of questions. #multiomics #methylation #singlecell #constellation
#uqccr #uq represent! Prof Salomon wowing the crowd again #multiomics2024 #evs#extracellularvesicles #ovariancancer
Yesterday, I presented our latest results mining single molecule m6A data at #multiomics2024:
We see changes in m6A levels following CRISPR-cas13 knock-down of oncogenic lncRNAs that are independent of mRNA expression levels, revealing new dimensions of highly pertinent biological information
I think it’s neck and neck as to the number of posts on Twitter V BlueSky for #Multiomics2024 Interesting to watch @aruthak.bsky.social @jasmineplummer.bsky.social @amymccart.bsky.social
#Multiomics #Spatialbiology #SingleCell #genomics
Amazing end to Day1 of #multiomics2024 Already excited for tomorrow’s talks #brisbane #breastcancer #metaplastic
When you get to be your own caricature!! Grateful to be in #oz sharing science with #friends. For those missing out #multiomics2024 is where #singlecell #spatial #omics #immunology #oncology and #precisionmedicine is where it’s at🥳
Tumors: an ecosystem of interacting and inter-dependent cellular communities: "seed vs soil" Define complexity of the TME @single cell-spatial resolution Composition (identities) Lineage and dynamics Gene regulatory networks (GRNS) Specialized functions Cell-cell interactions (tumor-stroma-immune) Investigate mechanisms of cross-regulatory interactions drive cancer cell plasticity define intra-tumor phenotypic heterogeneity modulate therapy response
It is not enough to study cancer cells in isolation. Tumors are an ecosystem of interacting & inter-dependent cellular communities: "seed vs soil."
#multiomics2024 5/
Summary part 1 Sub-populations of cells have distinct combinations of oncogenes: In DLBCL, MYC+BCL2+BCL6-cells have the strongest impact on survival BCL6 negativity is important to assess (possible role of CCND2) A single-cell resolved definition of DEL lymphoma as a "continuum"; extrapolatable from IHC images Does this phenomenon (clinically relevant co-expression of oncogenes in a subset of cells) occur in other cancers? CSI SINGAPORE CANCER SCIENCE INSTITUTE OF SINGAPORE National University of Singapore Hoppe et al, Cancer Discovery 2023 RESEARCH ARTICLE Patterns of Oncogene Coexpression at Single-Cell Resolution Influence Survival in Lymphoma AACR JOURNALS 14
Many cancers have multiple oncogenes. Does it matter if they are in the same cell or not?
-- Anand Jeyesekaran kicking off the Immuno-Oncology session for #multiomics2024 🧵 1/
bulk RNA-seq: a big pile of different colored legs all mixed up single-cell RNA-seq: small piles of Legos organized by color spatial transcriptomics: image of the brain built out of Legos
An analogy of the differences between bulk RNA-seq, single-cell RNA-seq, and spatial transcriptomics
#multiomics2024 10/
Very recent research (from the same author) taking advantage of larger datasets & a novel vision transformer architecture:
www.nature.com/articles/s41... Bifulco, #multiomics2024 5/
