Karyopherins remodel the dynamic organization of the nuclear pore complex transport barrier - Nature Cell Biology Kozai, Fernandez-Martinez et al. use high-speed atomic force microscopy to study the permeability barrier of yeast nuclear pore complexes. They show that karyopherins remodel a central plug that shape...

☕Kozai, Fernandez-Martinez et al. use high-speed atomic force #microscopy to study the permeability barrier of yeast #NuclearPore complexes. They show that #karyopherins remodel a central plug that shapes barrier dynamics and disorder within the pore.
bit.ly/4p9LXGS

DNA-PAINT and RESI imaging of ALFA-tagged nuclear pore protein Nup96 using the RESI anti-ALFA kit. For RESI imaging, four Exchange-PAINT rounds were performed using a mixture of anti-ALFA single-domain antibodies carrying docking strands F1, F2, F3, and F4 (10,000 frames per round,100 ms integration time, ~ 80 min total imaging time). Image post-processing was carried out using the open-source DNA-PAINT analysis software Picasso's RESI module. For classic DNA-PAINT, all four imager strands were added to the sample simultaneously.

DNA-PAINT and RESI imaging of ALFA-tagged nuclear pore protein Nup96 using the RESI anti-ALFA kit. For RESI imaging, four Exchange-PAINT rounds were performed using a mixture of anti-ALFA single-domain antibodies carrying docking strands F1, F2, F3, and F4 (10,000 frames per round,100 ms integration time, ~ 80 min total imaging time). Image post-processing was carried out using the open-source DNA-PAINT analysis software Picasso's RESI module. For classic DNA-PAINT, all four imager strands were added to the sample simultaneously.

🚀 New Product Launch! 🚀
Ready to push your imaging to 𝘀𝘂𝗯-𝗻𝗮𝗻𝗼𝗺𝗲𝘁𝗲𝗿 𝘀𝗽𝗮𝘁𝗶𝗮𝗹 𝗿𝗲𝘀𝗼𝗹𝘂𝘁𝗶𝗼𝗻? Introducing our newest 𝗥𝗘𝗦𝗜 𝗞𝗶𝘁 𝗳𝗼𝗿 𝗔𝗟𝗙𝗔-𝘁𝗮𝗴𝗴𝗲𝗱 𝗽𝗿𝗼𝘁𝗲𝗶𝗻𝘀!
🔗 www.massive-photonics.com/product-cate...

#SMLM #RESI #DNAPAINT #Microscopy #nuclearpore

💯🔬Fascination #cells: Edvinas Stankunas from @maxperutzlabs.bsky.social & #MedUniVienna is researching a central building block of the #nuclearpore. Aim: to reconstruct the complex in the laboratory in order to better understand malfunctions and disease mechanisms. ➡️ bit.ly/3YyiJa6

Nuclear pores safeguard the integrity of the nuclear envelope - Nature Cell Biology Taniguchi et al. structurally analyse nuclear pore complex architecture in situ during differentiation, which is associated with mechanical constraints on the nuclear envelope. They link nuclear pore ...

☕Taniguchi et al. structurally analyse #NuclearPore complex architecture in situ during differentiation, which is associated with mechanical constraints on the nuclear envelope. They link nuclear pore complex elasticity to nuclear envelope integrity in differentiation.
go.nature.com/42PJurY

Paper visualizing traffic through nuclear pores unlocks the full potential of #MINFLUX:
2-color 3D tracking with sub-ms time resolution and single-digit nm localization precision 💪

Congrats @abhishek-sau.bsky.social, @musser-lab.bsky.social, @thebioguy.bsky.social, @EMBL, et al!
#SMLM #nuclearpore

Exportin-1 functions as an adaptor for transcription factor-mediated docking of chromatin at the nuclear pore complex Many genes physically interact with the nuclear pore complex (NPC), which requires transcription factors (TFs). Ge et al. show that the exportin Crm1 binds TFs and nuclear pore proteins to dock chroma...

Cool! New role for #Exportin1 #Crm1 #Xpo1
#Adaptor #NuclearPore #NPC #TranscriptionFactor

Exportin-1 functions as an adaptor for transcription factor-mediated docking of chromatin at the nuclear pore complex: Molecular Cell www.cell.com/molecular-ce...

Conformational dynamics of the nuclear pore complex central channel The nuclear pore complex (NPC) is a vital regulator of molecular transport between the nucleus and cytoplasm in eukaryotic cells. At the heart of the NPC’s function are intrinsically disordered phenyl...

🚨New Review Alert!🚨How do disordered FG-Nups regulate nuclear transport?🍝🐍 Our latest review explores recent advances in imaging & modeling, linking NPC function to disease. #NuclearPore #FGNups #Biophysics 👉
portlandpress.com/biochemsoctr...

Opening the gate: Complexity and modularity of the nuclear pore scaffold and basket Nuclear pore complexes (NPCs) are giant molecular assemblies that form the gateway between the nucleus and the cytoplasm and accommodate the bidirecti…

#NuclearPore #NPC

www.sciencedirect.com/science/arti...

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Large #molecules need more help to travel through a #NuclearPore into the #CellNucleus www.uni-mainz.de/presse/aktuell/11905_ENG... #biophysics #biochemistry #biomolecules @LemkeLab
@imbmainz @embl @UofT

Abb./©: Giulia Paci (CC BY 4.0)